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The map label for this gene is nusB

Identifier: 91788748

GI number: 91788748

Start: 3047427

End: 3047990

Strand: Direct

Name: nusB

Synonym: Bpro_2887

Alternate gene names: 91788748

Gene position: 3047427-3047990 (Clockwise)

Preceding gene: 91788747

Following gene: 91788749

Centisome position: 58.6

GC content: 59.04

Gene sequence:

>564_bases
TTGCATAAGCCCCTGTTTCAAAAAACCTTTATTACCAACCGGCGCTTCATGGCTGAACCCCTCAACAAACCCAAACGACC
ACCCCAAATCCGCACCGGCCTGACCGACACGGGTGTCAAAAAAGCGGCTGACAAATCGGCGCGTACCCGGGCGCGCGAAT
TCGCCCTGCAGGCGCTGTACCAGCATCTGGTGGGCCGTAATGACGCCGACTCGATTGATGCCTTTACCCGCGACCTGGTC
GGTTTTCACAAAGCAGACTCGGCGCACTACGACGCGCTACTGCATGGCTGCATTGAGGGCGCCAGCGCCCTTGACGCGGT
CATTACCCCCCTGCTGGATCGGCCCATGGCCGAAATTTCACCGGTTGAACATGCCGTGCTCTGGATGGGCGCTTACGAAT
TCAGGCAATGCCTGGATGTCCCCTACCGCGTCGTGATCAACGAGTGCATCGAGCTGGCCAAGGCCTTCGGCGGCACCGAC
GGGCACAAATACGTCAACGGAGTCCTGCATAAAATGGCACCCTCCCTGCGCGCCGCCGAAGTTGCCGCTGACCAGACAAA
ATAA

Upstream 100 bases:

>100_bases
TTGACGACCGAGAACCTGGCGCAGGCCGAGGCGCGCCAGATTGAAAAAGGGCGTGACGCGGCGCGCGTTGCCGTCGAAAT
GGCCAATCTGCTGGACGAGC

Downstream 100 bases:

>100_bases
ATCGCAGACCATGAAAGGCTTGCCGTGAAAATCGCCCAGCGGGCTCAGCGGATTGAGCCGTTTTACGTCATGGAGGTTGC
CAAGGCAGCCTCGGCGCTAG

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MHKPLFQKTFITNRRFMAEPLNKPKRPPQIRTGLTDTGVKKAADKSARTRAREFALQALYQHLVGRNDADSIDAFTRDLV
GFHKADSAHYDALLHGCIEGASALDAVITPLLDRPMAEISPVEHAVLWMGAYEFRQCLDVPYRVVINECIELAKAFGGTD
GHKYVNGVLHKMAPSLRAAEVAADQTK

Sequences:

>Translated_187_residues
MHKPLFQKTFITNRRFMAEPLNKPKRPPQIRTGLTDTGVKKAADKSARTRAREFALQALYQHLVGRNDADSIDAFTRDLV
GFHKADSAHYDALLHGCIEGASALDAVITPLLDRPMAEISPVEHAVLWMGAYEFRQCLDVPYRVVINECIELAKAFGGTD
GHKYVNGVLHKMAPSLRAAEVAADQTK
>Mature_187_residues
MHKPLFQKTFITNRRFMAEPLNKPKRPPQIRTGLTDTGVKKAADKSARTRAREFALQALYQHLVGRNDADSIDAFTRDLV
GFHKADSAHYDALLHGCIEGASALDAVITPLLDRPMAEISPVEHAVLWMGAYEFRQCLDVPYRVVINECIELAKAFGGTD
GHKYVNGVLHKMAPSLRAAEVAADQTK

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=125, Percent_Identity=41.6, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_POLSJ (Q129J0)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549700.1
- ProteinModelPortal:   Q129J0
- SMR:   Q129J0
- STRING:   Q129J0
- GeneID:   4014462
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2887
- NMPDR:   fig|296591.1.peg.5477
- eggNOG:   COG0781
- HOGENOM:   HBG734409
- OMA:   IPYKVVI
- PhylomeDB:   Q129J0
- ProtClustDB:   PRK00202
- BioCyc:   PSP296591:BPRO_2887-MONOMER
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 20754; Mature: 20754

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKPLFQKTFITNRRFMAEPLNKPKRPPQIRTGLTDTGVKKAADKSARTRAREFALQALY
CCCCHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QHLVGRNDADSIDAFTRDLVGFHKADSAHYDALLHGCIEGASALDAVITPLLDRPMAEIS
HHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
PVEHAVLWMGAYEFRQCLDVPYRVVINECIELAKAFGGTDGHKYVNGVLHKMAPSLRAAE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
VAADQTK
HHHCCCC
>Mature Secondary Structure
MHKPLFQKTFITNRRFMAEPLNKPKRPPQIRTGLTDTGVKKAADKSARTRAREFALQALY
CCCCHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QHLVGRNDADSIDAFTRDLVGFHKADSAHYDALLHGCIEGASALDAVITPLLDRPMAEIS
HHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
PVEHAVLWMGAYEFRQCLDVPYRVVINECIELAKAFGGTDGHKYVNGVLHKMAPSLRAAE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
VAADQTK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA