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The map label for this gene is gatA [H]

Identifier: 91788558

GI number: 91788558

Start: 2836945

End: 2838318

Strand: Direct

Name: gatA [H]

Synonym: Bpro_2696

Alternate gene names: 91788558

Gene position: 2836945-2838318 (Clockwise)

Preceding gene: 91788537

Following gene: 91788559

Centisome position: 54.55

GC content: 66.89

Gene sequence:

>1374_bases
ATGCACACCTCCAGCGCTCTTCCCGCCAACATTCACGTCACGCGCCACGCCCTGAGAACGGGCCAGGCCAGCCCATCGGA
AATCGTCGAGCGCGCCACCAGCATCGCCCAAAGCGAGGTCTGCCGGTATGTATTCATGCCAGGCAGCCTTGCAGCCAGCC
CGTTTTTGACCGCCAAAACACCCCAGGCCGAGCCTGTTGGAGCGGCCTTCAATGACCCGGCTCTGCCCCTGGCCGGCATT
CCCGTGTCGATCAAGGACCTGTTTGACGTGGCCGGCCAGACCACCGCCGCCGGCTCGACCGTGCTCGCCAACGCCCGGCC
CGCCGCCGAAGACTGCCCCGCCGTTGCGCGGTTGCGAGCGGCAGGTGCCGTGATTGCCGGCCGCACCAATATGGTGGAAT
TTGCCTTCTCGGGCGTGGGCATCAACCCGCATTACGGCACCCCTGTCAATCCGGCAGACACCGCCACAGAGCGGATCCCG
GGCGGCTCCTCGTCAGGCGCCGCCGTGTCGGTTGCCACAGGGGCCGCCATGGTGGGGCTTGGGTCAGACACGGGCGGTTC
AATCCGCATCCCGGCGGCGCTGTGCGGCATTGTGGGTTTTAAAAGCACGGCGCGTCTGGTGCCAACCAGCGGCGCCGTGC
CGCTGTCCACCAGCCTGGACACGGTATGCGCTCTGACGCGTTCAGTAAGCGACGCAATCACCGTGCATGAGGTACTGGCG
GCGCGCACCGTGACTCTGGCTGGCAAGCCCCTGTCCGCCTGCCGGCTCGCGGTGGCCCGCACCCAGATGCAGGATGGCCT
GGACAGCACCGTCGCCAGTGCGTTCGAGCGGAGCCTGCGCGTGTTGCGCCAGGCCGGTGCGCGCATCGAGGAAATCGCAC
TCGAAGAAATCAACGAACTGCCTGCCATCAATGCGACCGGGGGACTCTCGGCTGCCGAGAGCTATGCCTGGCACCGCACG
CTGATCGCCGCGCACCAGGCCGAGTACGACCCCCGGGTAGCGCTGCGCATCCTGCGTGGCGCCCAGATGAGCGCGGCCGA
CTACATTGACCTTGTCGCCGCCCGCCGGCAATGGATCGTCCGCATGGAAGCCCGGCTGGGCGGCTTTGACGCAGTACTCT
CCCCCACCGTGCCCCTCGTCGCACCGGCAATCGCCAGTGCGCTGCACGATGACGAGGAATTCTTCCGGGTCAATGGGCTT
TTGCTCCGCAATACGGCCGTGGTCAACATGCTCGACGGTTGCGCGATTTCGCTGCCTTGCCATACGCCCGGCCAGTTGCC
GGTCGGCCTGATGCTCTGGCACGCAGCACTGCACGACGACCCGGTGCTGGACCTGGCGCTGCAGGTCGAAGCGGCCCTGG
CTGGTTCACTGTAA

Upstream 100 bases:

>100_bases
TGGCTGATTTGCGATTGACTTTGCGAACAACAAAATCGTCATTTGCAAAGCCTGTCGAGTATAACACTCTCCTGCCCCGC
CCTCTTACCCACTCACCCAG

Downstream 100 bases:

>100_bases
AAACAGTCGCTGGCTGGCCCCGCTATATTGGGCTTGCAACCCGCTTTACCGATAAAACAATGCACACCAAGATTTCCCGC
TACCCCATCGCCATCACCAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MHTSSALPANIHVTRHALRTGQASPSEIVERATSIAQSEVCRYVFMPGSLAASPFLTAKTPQAEPVGAAFNDPALPLAGI
PVSIKDLFDVAGQTTAAGSTVLANARPAAEDCPAVARLRAAGAVIAGRTNMVEFAFSGVGINPHYGTPVNPADTATERIP
GGSSSGAAVSVATGAAMVGLGSDTGGSIRIPAALCGIVGFKSTARLVPTSGAVPLSTSLDTVCALTRSVSDAITVHEVLA
ARTVTLAGKPLSACRLAVARTQMQDGLDSTVASAFERSLRVLRQAGARIEEIALEEINELPAINATGGLSAAESYAWHRT
LIAAHQAEYDPRVALRILRGAQMSAADYIDLVAARRQWIVRMEARLGGFDAVLSPTVPLVAPAIASALHDDEEFFRVNGL
LLRNTAVVNMLDGCAISLPCHTPGQLPVGLMLWHAALHDDPVLDLALQVEAALAGSL

Sequences:

>Translated_457_residues
MHTSSALPANIHVTRHALRTGQASPSEIVERATSIAQSEVCRYVFMPGSLAASPFLTAKTPQAEPVGAAFNDPALPLAGI
PVSIKDLFDVAGQTTAAGSTVLANARPAAEDCPAVARLRAAGAVIAGRTNMVEFAFSGVGINPHYGTPVNPADTATERIP
GGSSSGAAVSVATGAAMVGLGSDTGGSIRIPAALCGIVGFKSTARLVPTSGAVPLSTSLDTVCALTRSVSDAITVHEVLA
ARTVTLAGKPLSACRLAVARTQMQDGLDSTVASAFERSLRVLRQAGARIEEIALEEINELPAINATGGLSAAESYAWHRT
LIAAHQAEYDPRVALRILRGAQMSAADYIDLVAARRQWIVRMEARLGGFDAVLSPTVPLVAPAIASALHDDEEFFRVNGL
LLRNTAVVNMLDGCAISLPCHTPGQLPVGLMLWHAALHDDPVLDLALQVEAALAGSL
>Mature_457_residues
MHTSSALPANIHVTRHALRTGQASPSEIVERATSIAQSEVCRYVFMPGSLAASPFLTAKTPQAEPVGAAFNDPALPLAGI
PVSIKDLFDVAGQTTAAGSTVLANARPAAEDCPAVARLRAAGAVIAGRTNMVEFAFSGVGINPHYGTPVNPADTATERIP
GGSSSGAAVSVATGAAMVGLGSDTGGSIRIPAALCGIVGFKSTARLVPTSGAVPLSTSLDTVCALTRSVSDAITVHEVLA
ARTVTLAGKPLSACRLAVARTQMQDGLDSTVASAFERSLRVLRQAGARIEEIALEEINELPAINATGGLSAAESYAWHRT
LIAAHQAEYDPRVALRILRGAQMSAADYIDLVAARRQWIVRMEARLGGFDAVLSPTVPLVAPAIASALHDDEEFFRVNGL
LLRNTAVVNMLDGCAISLPCHTPGQLPVGLMLWHAALHDDPVLDLALQVEAALAGSL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=26.4112903225806, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI195972892, Length=144, Percent_Identity=38.1944444444444, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI166795287, Length=135, Percent_Identity=34.8148148148148, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=412, Percent_Identity=25.9708737864078, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=244, Percent_Identity=31.5573770491803, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=330, Percent_Identity=26.969696969697, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=126, Percent_Identity=36.5079365079365, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=312, Percent_Identity=29.8076923076923, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=312, Percent_Identity=29.8076923076923, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=224, Percent_Identity=29.9107142857143, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=227, Percent_Identity=30.8370044052863, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=391, Percent_Identity=30.4347826086957, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=155, Percent_Identity=37.4193548387097, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648113, Length=294, Percent_Identity=30.952380952381, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45550774, Length=161, Percent_Identity=37.2670807453416, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648435, Length=161, Percent_Identity=37.2670807453416, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648437, Length=161, Percent_Identity=37.2670807453416, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648441, Length=161, Percent_Identity=37.2670807453416, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648439, Length=161, Percent_Identity=37.2670807453416, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161078093, Length=424, Percent_Identity=26.1792452830189, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644968, Length=433, Percent_Identity=24.4803695150115, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356731, Length=175, Percent_Identity=37.1428571428571, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24652985, Length=425, Percent_Identity=25.6470588235294, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922090, Length=425, Percent_Identity=25.6470588235294, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652981, Length=425, Percent_Identity=25.6470588235294, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652983, Length=425, Percent_Identity=25.6470588235294, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47212; Mature: 47212

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTSSALPANIHVTRHALRTGQASPSEIVERATSIAQSEVCRYVFMPGSLAASPFLTAKT
CCCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEECCC
PQAEPVGAAFNDPALPLAGIPVSIKDLFDVAGQTTAAGSTVLANARPAAEDCPAVARLRA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
AGAVIAGRTNMVEFAFSGVGINPHYGTPVNPADTATERIPGGSSSGAAVSVATGAAMVGL
HCEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCEEEEEC
GSDTGGSIRIPAALCGIVGFKSTARLVPTSGAVPLSTSLDTVCALTRSVSDAITVHEVLA
CCCCCCCEECHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARTVTLAGKPLSACRLAVARTQMQDGLDSTVASAFERSLRVLRQAGARIEEIALEEINEL
HHHEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
PAINATGGLSAAESYAWHRTLIAAHQAEYDPRVALRILRGAQMSAADYIDLVAARRQWIV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RMEARLGGFDAVLSPTVPLVAPAIASALHDDEEFFRVNGLLLRNTAVVNMLDGCAISLPC
HHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHEECCEEEECHHHHHHHCCCEEECCC
HTPGQLPVGLMLWHAALHDDPVLDLALQVEAALAGSL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTSSALPANIHVTRHALRTGQASPSEIVERATSIAQSEVCRYVFMPGSLAASPFLTAKT
CCCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEECCC
PQAEPVGAAFNDPALPLAGIPVSIKDLFDVAGQTTAAGSTVLANARPAAEDCPAVARLRA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
AGAVIAGRTNMVEFAFSGVGINPHYGTPVNPADTATERIPGGSSSGAAVSVATGAAMVGL
HCEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCEEEEEC
GSDTGGSIRIPAALCGIVGFKSTARLVPTSGAVPLSTSLDTVCALTRSVSDAITVHEVLA
CCCCCCCEECHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARTVTLAGKPLSACRLAVARTQMQDGLDSTVASAFERSLRVLRQAGARIEEIALEEINEL
HHHEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
PAINATGGLSAAESYAWHRTLIAAHQAEYDPRVALRILRGAQMSAADYIDLVAARRQWIV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RMEARLGGFDAVLSPTVPLVAPAIASALHDDEEFFRVNGLLLRNTAVVNMLDGCAISLPC
HHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHEECCEEEECHHHHHHHCCCEEECCC
HTPGQLPVGLMLWHAALHDDPVLDLALQVEAALAGSL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA