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The map label for this gene is lpxD

Identifier: 91788547

GI number: 91788547

Start: 2825426

End: 2826481

Strand: Reverse

Name: lpxD

Synonym: Bpro_2685

Alternate gene names: 91788547

Gene position: 2826481-2825426 (Counterclockwise)

Preceding gene: 91788548

Following gene: 91788546

Centisome position: 54.35

GC content: 61.74

Gene sequence:

>1056_bases
GTGACGCTCCGTCTGGCCGATATTGTTCAGTCCCTGGCCCCAACGGTGCAGGCCGAGCTCGTCGGCAACCCCGATGTGCA
GATAGAGCGGCTGTCCACGCTGGACGCGGCCGGCCCCCACGACCTGAGTTTTCTCAGCCACCCCAAATATCTCGCCAAAC
TTGCGCAGTCGGCTGCCGCCTGTGTCGTGGTGGCTCCGGCTGCCCGAGAGGCCGCTGTCAGCCGCGGTGCCTGCATCGTG
GTGGACGACCCCTACCATTACTTTGCCCTGATCACCCAGCTCTGGAAGTACCGGCATGTGTTGCAGGCTGCGCCGCACAT
TCATCCCAGCGCGTTTATCGATCCCGCCGCAACCCTCGCGCCGGGTGTTTCCGTGGGCGCGTTCGCCTGCATCTCGGCCG
GCACTGTGATCGGCGCGGGCGCGCGGATTGCAGAGCATTGCGTTATTGGGCGGGATGCGCATGTTGGTGCCGAAAGCCGG
CTGTCGGCCCGCGTGACCGTCGCAGATGGCTGCTACATCGGCGAGCGTTGCATTGTGCATCCCGGTGCGGTGATCGGCGC
GGACGGTTTTGGCTTCGCACCGCATCAGGGTCAATGGATCAAGATCGAGCAGCTTGGCGCGGTGAAGATCGGCAATGATG
TGGAAATCGGCGCCAACACATGCATTGACCGGGGCGCGCTGCAAGATACGGTTCTGGAAGACGGTGTCAAGCTGGATAAC
CTGGTGCAGATAGGCCACAACGTGCGGATCGGCAAGCACACCGCCATGGCAGGTTGCGCGGGTGTCGCGGGCAGTGCCAC
CATCGGCGCGCATTGCACGGTGGGCGGAGGCGCCATTGTGCTGGGCCACCTGAGCCTGGCTGATCACGTGCATATTTCGG
CGGCGTCGGTGGTGACGCGGTCCATTTTGAAGCCTGGTCATTACACCGGTCTCTTTCCTATCGACGACAATACCGCGTGG
GAAAAAAATGCTGCCACGCTCAAGCAGCTTCACGTCTTGCGGGAAAGACTCAAACAGGCAGAAAAATCGCTGTCGAACAT
TCAAGAAAAATCATGA

Upstream 100 bases:

>100_bases
AGCATGACATCACCGACAAGGTGATCAAGGCGCTGAACGCCTCCCGTTAATTGCGGGGTGTGTTCACCACCAGCATCGTT
GTGAAAATGGCAGGCGCAGT

Downstream 100 bases:

>100_bases
TGGATATTCACCAAATTCTCAAGCAGCTGCCGCACCGGTACCCGATTCTGCTGGTCGACCGTGTGCTCGAAATCGAAAAG
GGAAAACGAATCAAGGCCCT

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTLRLADIVQSLAPTVQAELVGNPDVQIERLSTLDAAGPHDLSFLSHPKYLAKLAQSAAACVVVAPAAREAAVSRGACIV
VDDPYHYFALITQLWKYRHVLQAAPHIHPSAFIDPAATLAPGVSVGAFACISAGTVIGAGARIAEHCVIGRDAHVGAESR
LSARVTVADGCYIGERCIVHPGAVIGADGFGFAPHQGQWIKIEQLGAVKIGNDVEIGANTCIDRGALQDTVLEDGVKLDN
LVQIGHNVRIGKHTAMAGCAGVAGSATIGAHCTVGGGAIVLGHLSLADHVHISAASVVTRSILKPGHYTGLFPIDDNTAW
EKNAATLKQLHVLRERLKQAEKSLSNIQEKS

Sequences:

>Translated_351_residues
MTLRLADIVQSLAPTVQAELVGNPDVQIERLSTLDAAGPHDLSFLSHPKYLAKLAQSAAACVVVAPAAREAAVSRGACIV
VDDPYHYFALITQLWKYRHVLQAAPHIHPSAFIDPAATLAPGVSVGAFACISAGTVIGAGARIAEHCVIGRDAHVGAESR
LSARVTVADGCYIGERCIVHPGAVIGADGFGFAPHQGQWIKIEQLGAVKIGNDVEIGANTCIDRGALQDTVLEDGVKLDN
LVQIGHNVRIGKHTAMAGCAGVAGSATIGAHCTVGGGAIVLGHLSLADHVHISAASVVTRSILKPGHYTGLFPIDDNTAW
EKNAATLKQLHVLRERLKQAEKSLSNIQEKS
>Mature_350_residues
TLRLADIVQSLAPTVQAELVGNPDVQIERLSTLDAAGPHDLSFLSHPKYLAKLAQSAAACVVVAPAAREAAVSRGACIVV
DDPYHYFALITQLWKYRHVLQAAPHIHPSAFIDPAATLAPGVSVGAFACISAGTVIGAGARIAEHCVIGRDAHVGAESRL
SARVTVADGCYIGERCIVHPGAVIGADGFGFAPHQGQWIKIEQLGAVKIGNDVEIGANTCIDRGALQDTVLEDGVKLDNL
VQIGHNVRIGKHTAMAGCAGVAGSATIGAHCTVGGGAIVLGHLSLADHVHISAASVVTRSILKPGHYTGLFPIDDNTAWE
KNAATLKQLHVLRERLKQAEKSLSNIQEKS

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=344, Percent_Identity=39.2441860465116, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_POLSJ (Q12A41)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549499.1
- ProteinModelPortal:   Q12A41
- STRING:   Q12A41
- GeneID:   4014640
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2685
- NMPDR:   fig|296591.1.peg.403
- eggNOG:   COG1044
- HOGENOM:   HBG469615
- OMA:   CFVGKNT
- PhylomeDB:   Q12A41
- ProtClustDB:   PRK00892
- BioCyc:   PSP296591:BPRO_2685-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36658; Mature: 36527

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLADIVQSLAPTVQAELVGNPDVQIERLSTLDAAGPHDLSFLSHPKYLAKLAQSAAA
CCEEHHHHHHHHCCHHEEEECCCCCCEEEEEHHHCCCCCCCCHHHCCHHHHHHHHHCCCE
CVVVAPAAREAAVSRGACIVVDDPYHYFALITQLWKYRHVLQAAPHIHPSAFIDPAATLA
EEEEECCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHC
PGVSVGAFACISAGTVIGAGARIAEHCVIGRDAHVGAESRLSARVTVADGCYIGERCIVH
CCCCHHHHHHHHCCCEECCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEECCEEEEC
PGAVIGADGFGFAPHQGQWIKIEQLGAVKIGNDVEIGANTCIDRGALQDTVLEDGVKLDN
CCCEECCCCCCCCCCCCCEEEEEECCEEEECCCCEECCHHHHCCCCHHHHHHHCCCCHHH
LVQIGHNVRIGKHTAMAGCAGVAGSATIGAHCTVGGGAIVLGHLSLADHVHISAASVVTR
HHHHCCCEEECCHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEEHHHHHEEHHHHHHHHH
SILKPGHYTGLFPIDDNTAWEKNAATLKQLHVLRERLKQAEKSLSNIQEKS
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLRLADIVQSLAPTVQAELVGNPDVQIERLSTLDAAGPHDLSFLSHPKYLAKLAQSAAA
CEEHHHHHHHHCCHHEEEECCCCCCEEEEEHHHCCCCCCCCHHHCCHHHHHHHHHCCCE
CVVVAPAAREAAVSRGACIVVDDPYHYFALITQLWKYRHVLQAAPHIHPSAFIDPAATLA
EEEEECCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHC
PGVSVGAFACISAGTVIGAGARIAEHCVIGRDAHVGAESRLSARVTVADGCYIGERCIVH
CCCCHHHHHHHHCCCEECCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEECCEEEEC
PGAVIGADGFGFAPHQGQWIKIEQLGAVKIGNDVEIGANTCIDRGALQDTVLEDGVKLDN
CCCEECCCCCCCCCCCCCEEEEEECCEEEECCCCEECCHHHHCCCCHHHHHHHCCCCHHH
LVQIGHNVRIGKHTAMAGCAGVAGSATIGAHCTVGGGAIVLGHLSLADHVHISAASVVTR
HHHHCCCEEECCHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEEHHHHHEEHHHHHHHHH
SILKPGHYTGLFPIDDNTAWEKNAATLKQLHVLRERLKQAEKSLSNIQEKS
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA