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The map label for this gene is rnhB

Identifier: 91788543

GI number: 91788543

Start: 2822407

End: 2823006

Strand: Reverse

Name: rnhB

Synonym: Bpro_2681

Alternate gene names: 91788543

Gene position: 2823006-2822407 (Counterclockwise)

Preceding gene: 91788544

Following gene: 91788542

Centisome position: 54.29

GC content: 64.33

Gene sequence:

>600_bases
TTGAAAGCTGAGCAAACTCCCTTGTCCTGGGACACTCCCGGTCTCCTGGCCGGCGTGGACGAGGCCGGGCGGGGCCCGCT
GATGGGCCCAGTGGTGGCTGCGGCTGTCATCCTCGACGAGCTCAATCCCATCAAGGGCCTGGCCGACTCCAAAAAACTGA
CGCCGCAGCGGCGCGAAGCGTTGTATGACGAAATCCGCGCCAAAGCGCTGTGCTGCTCAATTGCGCTGGCCACGGCTGAG
GAGATAGACCGGCTGAACATCCTGCAGGCCACCATGCTGGCGATGAGGCGCGCGGTGGAGAGCCTGCGCCTGAAGCCCAA
CAAGGTGCTGGTCGACGGCAATCGCTTGCCCACGCTGGCCATCGTGTCGGAGGCCGTGATCGGAGGCGATGCCATCGTGC
CGGCCATCTCGGCCGCTTCGATTCTGGCCAAGGTCTACCGTGACCGCTGGTGCGCCGAGTTTCACCTCGAATATCCGCAG
TACGGTTTTGCCAGTCACAAAGGGTATTCCACAGCTGAACATCTCGCCGCGCTGCGCGAGCATGGCGCCTGTCCCCAGCA
CCGCCGGTCTTTTGCGCCGGTCGCCGAGGTCTTGCGGTGA

Upstream 100 bases:

>100_bases
CCTGCCAAAATAGCGGCTGTTGAACAACGATTCATGGCGCTGCATGCCGAACTGCAGCGCGATACTTCTCAATTGGCGAC
CGATGCGATCCAGCAAATTC

Downstream 100 bases:

>100_bases
GCGTCAGTCCCGGCGCTGTCACGCACATCACGTCACGCGACAACACGCTCATCAAGGAATTGCGCAAGCTGTCGCAGGAC
AGCACGGCTTACCGCAAGCA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKAEQTPLSWDTPGLLAGVDEAGRGPLMGPVVAAAVILDELNPIKGLADSKKLTPQRREALYDEIRAKALCCSIALATAE
EIDRLNILQATMLAMRRAVESLRLKPNKVLVDGNRLPTLAIVSEAVIGGDAIVPAISAASILAKVYRDRWCAEFHLEYPQ
YGFASHKGYSTAEHLAALREHGACPQHRRSFAPVAEVLR

Sequences:

>Translated_199_residues
MKAEQTPLSWDTPGLLAGVDEAGRGPLMGPVVAAAVILDELNPIKGLADSKKLTPQRREALYDEIRAKALCCSIALATAE
EIDRLNILQATMLAMRRAVESLRLKPNKVLVDGNRLPTLAIVSEAVIGGDAIVPAISAASILAKVYRDRWCAEFHLEYPQ
YGFASHKGYSTAEHLAALREHGACPQHRRSFAPVAEVLR
>Mature_199_residues
MKAEQTPLSWDTPGLLAGVDEAGRGPLMGPVVAAAVILDELNPIKGLADSKKLTPQRREALYDEIRAKALCCSIALATAE
EIDRLNILQATMLAMRRAVESLRLKPNKVLVDGNRLPTLAIVSEAVIGGDAIVPAISAASILAKVYRDRWCAEFHLEYPQ
YGFASHKGYSTAEHLAALREHGACPQHRRSFAPVAEVLR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=61.4130434782609, Blast_Score=213, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_POLSJ (Q12A45)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549495.1
- ProteinModelPortal:   Q12A45
- SMR:   Q12A45
- STRING:   Q12A45
- GeneID:   4014674
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2681
- NMPDR:   fig|296591.1.peg.399
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   DEINILW
- PhylomeDB:   Q12A45
- ProtClustDB:   PRK00015
- BioCyc:   PSP296591:BPRO_2681-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21589; Mature: 21589

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAEQTPLSWDTPGLLAGVDEAGRGPLMGPVVAAAVILDELNPIKGLADSKKLTPQRREA
CCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHH
LYDEIRAKALCCSIALATAEEIDRLNILQATMLAMRRAVESLRLKPNKVLVDGNRLPTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
IVSEAVIGGDAIVPAISAASILAKVYRDRWCAEFHLEYPQYGFASHKGYSTAEHLAALRE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
HGACPQHRRSFAPVAEVLR
CCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKAEQTPLSWDTPGLLAGVDEAGRGPLMGPVVAAAVILDELNPIKGLADSKKLTPQRREA
CCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHH
LYDEIRAKALCCSIALATAEEIDRLNILQATMLAMRRAVESLRLKPNKVLVDGNRLPTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
IVSEAVIGGDAIVPAISAASILAKVYRDRWCAEFHLEYPQYGFASHKGYSTAEHLAALRE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
HGACPQHRRSFAPVAEVLR
CCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA