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The map label for this gene is rhtB [H]

Identifier: 91788525

GI number: 91788525

Start: 2799489

End: 2800124

Strand: Direct

Name: rhtB [H]

Synonym: Bpro_2663

Alternate gene names: 91788525

Gene position: 2799489-2800124 (Clockwise)

Preceding gene: 91788524

Following gene: 91788526

Centisome position: 53.83

GC content: 64.47

Gene sequence:

>636_bases
ATGACCCTCTCCGCCTGGCTCCTGTTCATCACCATTTCGCTGGTCACCGCATTCAGCCCCGGGCCGGGCATTTTGCTGGC
CGTCTCCAACGCCGTCGCCTGGGGGCCACGCAAGACGCTGTGCAGTTCTGCCGGCAATGTGCTGGGCGTGTTCGCCGTCT
CCGGCGCGGCAATGGCGGGCCTGGGCACCTTGCTGCACACCTCGGCCTGGCTGTTTGCGGTGCTCAAGATCTTGGGTGCG
GTGTACCTGGTCTACCTCGGTTACCGTCAATGGACGAGCAAAACCTCGATGTTCGACAAGCCTTTGCCCACCGAGGCGCA
GCCCGGAAAACCCAACGCCGTCCTGTTCCGGCAAGGCCTGCTGGTGGCGCTGACCAACCCTAAAAGCATCCTGTTCTTCA
CCGCGCTGTTTCCCCAGTTCATCCACCCGGACGCCCCGGTGCTGCCGCAATTCCTCGCGCTGACCAGCGCCTTCACCGCC
TGCGTACTGACATCCCACCTCGCCTACGTGCTGCTGGCAAAGCGCACCCACCGCTGGTTCGGCACGGCCCACCGGGCCCG
CCTGTTCAACCGTGTCTGCGGCGGCGCCTTCGGCCTGCTGGGTGTCAGCCTGTTGCGTTTAAAAACCCCCAACTGA

Upstream 100 bases:

>100_bases
CTCCGGGTCGAAGTACCAACGACTGCCTTGCTAAGGTAAATTCCACTCCTGCCATTTCTCATTTCCGATTTCTCATTTTC
GATTTCTCACCTCTGCGCCC

Downstream 100 bases:

>100_bases
ATGGCCGCACCGCGCAACGCCACCGGCGACTCGTTTTTTGCCTGGGACGGGGATGTGCTGGTGGTCAACATCCTGGGCAA
GCCCGCCGCCAGCCGCGACG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTLSAWLLFITISLVTAFSPGPGILLAVSNAVAWGPRKTLCSSAGNVLGVFAVSGAAMAGLGTLLHTSAWLFAVLKILGA
VYLVYLGYRQWTSKTSMFDKPLPTEAQPGKPNAVLFRQGLLVALTNPKSILFFTALFPQFIHPDAPVLPQFLALTSAFTA
CVLTSHLAYVLLAKRTHRWFGTAHRARLFNRVCGGAFGLLGVSLLRLKTPN

Sequences:

>Translated_211_residues
MTLSAWLLFITISLVTAFSPGPGILLAVSNAVAWGPRKTLCSSAGNVLGVFAVSGAAMAGLGTLLHTSAWLFAVLKILGA
VYLVYLGYRQWTSKTSMFDKPLPTEAQPGKPNAVLFRQGLLVALTNPKSILFFTALFPQFIHPDAPVLPQFLALTSAFTA
CVLTSHLAYVLLAKRTHRWFGTAHRARLFNRVCGGAFGLLGVSLLRLKTPN
>Mature_210_residues
TLSAWLLFITISLVTAFSPGPGILLAVSNAVAWGPRKTLCSSAGNVLGVFAVSGAAMAGLGTLLHTSAWLFAVLKILGAV
YLVYLGYRQWTSKTSMFDKPLPTEAQPGKPNAVLFRQGLLVALTNPKSILFFTALFPQFIHPDAPVLPQFLALTSAFTAC
VLTSHLAYVLLAKRTHRWFGTAHRARLFNRVCGGAFGLLGVSLLRLKTPN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=201, Percent_Identity=33.8308457711443, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788099, Length=128, Percent_Identity=35.15625, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1786522, Length=209, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI48994978, Length=145, Percent_Identity=28.9655172413793, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22671; Mature: 22540

Theoretical pI: Translated: 11.23; Mature: 11.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSAWLLFITISLVTAFSPGPGILLAVSNAVAWGPRKTLCSSAGNVLGVFAVSGAAMAG
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCHHHHHHCCCCEEEHHHHHHHHHHH
LGTLLHTSAWLFAVLKILGAVYLVYLGYRQWTSKTSMFDKPLPTEAQPGKPNAVLFRQGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCEEEEECCE
LVALTNPKSILFFTALFPQFIHPDAPVLPQFLALTSAFTACVLTSHLAYVLLAKRTHRWF
EEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTAHRARLFNRVCGGAFGLLGVSLLRLKTPN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLSAWLLFITISLVTAFSPGPGILLAVSNAVAWGPRKTLCSSAGNVLGVFAVSGAAMAG
CHHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCHHHHHHCCCCEEEHHHHHHHHHHH
LGTLLHTSAWLFAVLKILGAVYLVYLGYRQWTSKTSMFDKPLPTEAQPGKPNAVLFRQGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCEEEEECCE
LVALTNPKSILFFTALFPQFIHPDAPVLPQFLALTSAFTACVLTSHLAYVLLAKRTHRWF
EEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTAHRARLFNRVCGGAFGLLGVSLLRLKTPN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]