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The map label for this gene is paaF [C]

Identifier: 91788338

GI number: 91788338

Start: 2588037

End: 2588825

Strand: Reverse

Name: paaF [C]

Synonym: Bpro_2473

Alternate gene names: 91788338

Gene position: 2588825-2588037 (Counterclockwise)

Preceding gene: 91788340

Following gene: 91788337

Centisome position: 49.78

GC content: 69.46

Gene sequence:

>789_bases
ATGACAACAACAACTTACGCAGGCCTGCAACTGGAACTGCGCGGCCACACCGCCGTGCTGACCCTGGCCAACCCGCCCGC
CAACACCTGGACAGCGCAAAGCCTGGCCGCGCTCACGCAACTGGTGAGCGAGCTGAACGCCAGGCGCGAGATCTACAGCC
TGGTCGTCACCGGCCAGGGCGAGAAGTTTTTTTCGGCCGGCGCCGACCTCAAGCTGTTTGCCGATGGCGACAAGGCCGTG
GCGCGCGAGATGGCGCGCCGCTTCGGCGAGGCGTTTGAAGCGCTGGCCGGTTTTCGCGGCCTGTCGATTGCCGCGATCAA
CGGCTACGCCATGGGCGGCGGGCTCGAATGCGCGCTGGCCTGCGACCTGCGCATCGCCGAAGAGCAAGCCCAATTGGCGC
TGCCCGAAGCCGCTGTCGGCCTGTTGCCCTGCGCCGGCGGCACGCAGTGGCTGAGCTGGGCCGTGGGCGAAGCCTGGGCC
AAGCGCATGGTGCTGTGCGGCGAGCGCGTGGACGCGGCCACCGCGCTGCGCATCGGCCTGGTCGAAGAAGTCGTGCCGCG
CGGCCAGGCGTTGGCGCAGGCCCTGCTGCTGGCAGCGCGTGTGGAACAGCAAAGCCCGACCAGCGTGGCCGCCTGCAAGC
GCCTGATTCAGGGGGCGCGCACGCAGTCGCCCGCCGCCACCCTGGTGGCCGAGCGCGAGGCTTTTGTCGACTTGTTCGAC
AGCCAGGACCAGCGCGAAGGCGTGAGCGCCTTTCTGGCCAAGCGCGCGCCGGTCTGGCGCAATGCGTGA

Upstream 100 bases:

>100_bases
GCGTGCACCAGATCGTGGAAGGCACCAACGAAATCATGCGCGTGATCGTCTCGCGCCGCATGCTGGAAACCGACAACACA
GACAACACCGAGGAAATCCG

Downstream 100 bases:

>100_bases
AGGAAAATATGAACCCCCACGCTCACATTCGTTCGCTGCCCCGCGAGGGGGCTGAGTGCCGCGGCTCCGAGCCTGCCGAC
GCAGGCTTGGACGGCACGAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTTTTYAGLQLELRGHTAVLTLANPPANTWTAQSLAALTQLVSELNARREIYSLVVTGQGEKFFSAGADLKLFADGDKAV
AREMARRFGEAFEALAGFRGLSIAAINGYAMGGGLECALACDLRIAEEQAQLALPEAAVGLLPCAGGTQWLSWAVGEAWA
KRMVLCGERVDAATALRIGLVEEVVPRGQALAQALLLAARVEQQSPTSVAACKRLIQGARTQSPAATLVAEREAFVDLFD
SQDQREGVSAFLAKRAPVWRNA

Sequences:

>Translated_262_residues
MTTTTYAGLQLELRGHTAVLTLANPPANTWTAQSLAALTQLVSELNARREIYSLVVTGQGEKFFSAGADLKLFADGDKAV
AREMARRFGEAFEALAGFRGLSIAAINGYAMGGGLECALACDLRIAEEQAQLALPEAAVGLLPCAGGTQWLSWAVGEAWA
KRMVLCGERVDAATALRIGLVEEVVPRGQALAQALLLAARVEQQSPTSVAACKRLIQGARTQSPAATLVAEREAFVDLFD
SQDQREGVSAFLAKRAPVWRNA
>Mature_261_residues
TTTTYAGLQLELRGHTAVLTLANPPANTWTAQSLAALTQLVSELNARREIYSLVVTGQGEKFFSAGADLKLFADGDKAVA
REMARRFGEAFEALAGFRGLSIAAINGYAMGGGLECALACDLRIAEEQAQLALPEAAVGLLPCAGGTQWLSWAVGEAWAK
RMVLCGERVDAATALRIGLVEEVVPRGQALAQALLLAARVEQQSPTSVAACKRLIQGARTQSPAATLVAEREAFVDLFDS
QDQREGVSAFLAKRAPVWRNA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=33.203125, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI68989263, Length=205, Percent_Identity=34.1463414634146, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4502327, Length=237, Percent_Identity=29.1139240506329, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI20127408, Length=195, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=26.6393442622951, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=41.6666666666667, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=28.7292817679558, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=28.7292817679558, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=28.936170212766, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=28.936170212766, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI62530384, Length=238, Percent_Identity=26.0504201680672, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=40, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=34.7014925373134, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788682, Length=225, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=163, Percent_Identity=31.9018404907975, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082183, Length=240, Percent_Identity=24.5833333333333, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=24.390243902439, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787660, Length=244, Percent_Identity=31.1475409836066, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=35.408560311284, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=32.5301204819277, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=28.8288288288288, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=192, Percent_Identity=30.7291666666667, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=191, Percent_Identity=34.5549738219895, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=191, Percent_Identity=34.5549738219895, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=191, Percent_Identity=34.5549738219895, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=27.3542600896861, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=30.1960784313725, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=208, Percent_Identity=28.3653846153846, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=32.5757575757576, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=32.5757575757576, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=29.3650793650794, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24650670, Length=228, Percent_Identity=30.2631578947368, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=21.4285714285714, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357171, Length=96, Percent_Identity=41.6666666666667, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45550169, Length=258, Percent_Identity=23.6434108527132, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921018, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27802; Mature: 27671

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTYAGLQLELRGHTAVLTLANPPANTWTAQSLAALTQLVSELNARREIYSLVVTGQG
CCCCEEECEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
EKFFSAGADLKLFADGDKAVAREMARRFGEAFEALAGFRGLSIAAINGYAMGGGLECALA
CHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCEEEEE
CDLRIAEEQAQLALPEAAVGLLPCAGGTQWLSWAVGEAWAKRMVLCGERVDAATALRIGL
ECEEEHHHHHHHCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VEEVVPRGQALAQALLLAARVEQQSPTSVAACKRLIQGARTQSPAATLVAEREAFVDLFD
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
SQDQREGVSAFLAKRAPVWRNA
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTTTYAGLQLELRGHTAVLTLANPPANTWTAQSLAALTQLVSELNARREIYSLVVTGQG
CCCEEECEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
EKFFSAGADLKLFADGDKAVAREMARRFGEAFEALAGFRGLSIAAINGYAMGGGLECALA
CHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCEEEEE
CDLRIAEEQAQLALPEAAVGLLPCAGGTQWLSWAVGEAWAKRMVLCGERVDAATALRIGL
ECEEEHHHHHHHCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VEEVVPRGQALAQALLLAARVEQQSPTSVAACKRLIQGARTQSPAATLVAEREAFVDLFD
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
SQDQREGVSAFLAKRAPVWRNA
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA