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The map label for this gene is truB [H]

Identifier: 91788293

GI number: 91788293

Start: 2530305

End: 2531318

Strand: Direct

Name: truB [H]

Synonym: Bpro_2428

Alternate gene names: 91788293

Gene position: 2530305-2531318 (Clockwise)

Preceding gene: 91788292

Following gene: 91788294

Centisome position: 48.66

GC content: 63.81

Gene sequence:

>1014_bases
ATGACGCAAACCTCTACTCATCGCCAGAAGATTCAGCGACGTCCCGTGCACGGCGTGCTGCTGCTCGACAAGCCGCTGGG
CCTGTCGAGCAACCAGGCGCTGCAAAAAGCCAAATGGCTGTTGCGCGCCGACAAGGCCGGGCATACCGGTACGCTGGATC
CGCTGGCCACTGGCGTGCTGCCGCTGTGCTTTGGGGCGGCGACCAAATTCAGCCAGATCCAGTTGGACGCCGACAAGACG
TATGAAGCCGTGCTCCTGCTGGGCCGGAAAACCACGACGGCTGATGCCGAGGGCGACGTCATCGAGACGCGCCCCGTTCC
CGAGATCACCCCCGAACTGCTGGCAACCCTGACCCGGCGTTTTACCGGCCCGCTGGCGCAGATTCCGCCCATGTATTCCG
CGCTCAAGAAAGACGGCAAGGCACTGTACGAATACGCGCGCAAAGGCGAAGACGTGGAGCGGGAAGCGCGCCACATCACG
ATTTACAAGCTGGATATGGCTCTGACCCATGACGAGCGGGCGCCGGCAGCTATCAAAATCACCGTGACCTGCAGCAAGGG
CACCTACATTCGCACGCTTGGTGAAGACATCGGCGAGGCGATCGGTTGCGGCGCTCACCTCGGGTCCCTGAGGCGGCTGG
AGACCGGCGGCTATGTCGCTTCGCAGTGCGTGGGCCTGCCGGCGCTGGAAGCCATGAGCGAGCAGCAGCGCGAAGCCTGC
CTGCTGCCAGTTCAATCACTCGTCGCCAACTATCCGGTTGTCACACTCGATGCCGATAATGCAGGGCGCTTTTTGAGCGG
CCTGCGCCGTCGCGGCAGCCCCGGCCAATGGGGTCCGGACGCGGTGCTGGTGCAGGTGTACGGTAGCGACCCGGCGGCTT
TTCTCGGCTCGGCCCATGTCATGGCTGACGAGTTGATTCCGGGGCGCCTGCTCAGCCCGATTGAAATCCAGGACATGCTG
GCCCCGCGTCCGCCTGTTATTTCCAACCCTGCGGCAAGCGCCGCAACGGCTTAG

Upstream 100 bases:

>100_bases
AAGACGACTGAGTTCGCGCCGTTCCCCTTGACAGGTTCGGGGACAACGGTGCCAACGGAGCACGCGGGCGAGTCCCGTTC
GCTGAGCCTGTCGAAACTTT

Downstream 100 bases:

>100_bases
TGCCACCGATTTGAAGTTTTTAGAAAGCGAACCATGAGTCATAAGCAAATCCGCAACATCGCCATCATCGCCCACGTGGA
CCACGGCAAAACCACCATGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTQTSTHRQKIQRRPVHGVLLLDKPLGLSSNQALQKAKWLLRADKAGHTGTLDPLATGVLPLCFGAATKFSQIQLDADKT
YEAVLLLGRKTTTADAEGDVIETRPVPEITPELLATLTRRFTGPLAQIPPMYSALKKDGKALYEYARKGEDVEREARHIT
IYKLDMALTHDERAPAAIKITVTCSKGTYIRTLGEDIGEAIGCGAHLGSLRRLETGGYVASQCVGLPALEAMSEQQREAC
LLPVQSLVANYPVVTLDADNAGRFLSGLRRRGSPGQWGPDAVLVQVYGSDPAAFLGSAHVMADELIPGRLLSPIEIQDML
APRPPVISNPAASAATA

Sequences:

>Translated_337_residues
MTQTSTHRQKIQRRPVHGVLLLDKPLGLSSNQALQKAKWLLRADKAGHTGTLDPLATGVLPLCFGAATKFSQIQLDADKT
YEAVLLLGRKTTTADAEGDVIETRPVPEITPELLATLTRRFTGPLAQIPPMYSALKKDGKALYEYARKGEDVEREARHIT
IYKLDMALTHDERAPAAIKITVTCSKGTYIRTLGEDIGEAIGCGAHLGSLRRLETGGYVASQCVGLPALEAMSEQQREAC
LLPVQSLVANYPVVTLDADNAGRFLSGLRRRGSPGQWGPDAVLVQVYGSDPAAFLGSAHVMADELIPGRLLSPIEIQDML
APRPPVISNPAASAATA
>Mature_336_residues
TQTSTHRQKIQRRPVHGVLLLDKPLGLSSNQALQKAKWLLRADKAGHTGTLDPLATGVLPLCFGAATKFSQIQLDADKTY
EAVLLLGRKTTTADAEGDVIETRPVPEITPELLATLTRRFTGPLAQIPPMYSALKKDGKALYEYARKGEDVEREARHITI
YKLDMALTHDERAPAAIKITVTCSKGTYIRTLGEDIGEAIGCGAHLGSLRRLETGGYVASQCVGLPALEAMSEQQREACL
LPVQSLVANYPVVTLDADNAGRFLSGLRRRGSPGQWGPDAVLVQVYGSDPAAFLGSAHVMADELIPGRLLSPIEIQDMLA
PRPPVISNPAASAATA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=35.4545454545455, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI215599015, Length=199, Percent_Identity=31.1557788944724, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503337, Length=199, Percent_Identity=31.1557788944724, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=330, Percent_Identity=43.030303030303, Blast_Score=241, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17553978, Length=209, Percent_Identity=29.1866028708134, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=203, Percent_Identity=34.9753694581281, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=199, Percent_Identity=31.1557788944724, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=250, Percent_Identity=28.8, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364187, Length=250, Percent_Identity=28.8, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364185, Length=250, Percent_Identity=28.8, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364183, Length=250, Percent_Identity=28.8, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62471759, Length=250, Percent_Identity=28.8, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17975520, Length=250, Percent_Identity=28.8, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 36191; Mature: 36060

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTSTHRQKIQRRPVHGVLLLDKPLGLSSNQALQKAKWLLRADKAGHTGTLDPLATGVL
CCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
PLCFGAATKFSQIQLDADKTYEAVLLLGRKTTTADAEGDVIETRPVPEITPELLATLTRR
HHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
FTGPLAQIPPMYSALKKDGKALYEYARKGEDVEREARHITIYKLDMALTHDERAPAAIKI
HCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEEEEEEECCCCCCEEEEE
TVTCSKGTYIRTLGEDIGEAIGCGAHLGSLRRLETGGYVASQCVGLPALEAMSEQQREAC
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHH
LLPVQSLVANYPVVTLDADNAGRFLSGLRRRGSPGQWGPDAVLVQVYGSDPAAFLGSAHV
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHCCHHH
MADELIPGRLLSPIEIQDMLAPRPPVISNPAASAATA
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQTSTHRQKIQRRPVHGVLLLDKPLGLSSNQALQKAKWLLRADKAGHTGTLDPLATGVL
CCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
PLCFGAATKFSQIQLDADKTYEAVLLLGRKTTTADAEGDVIETRPVPEITPELLATLTRR
HHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
FTGPLAQIPPMYSALKKDGKALYEYARKGEDVEREARHITIYKLDMALTHDERAPAAIKI
HCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEEEEEEECCCCCCEEEEE
TVTCSKGTYIRTLGEDIGEAIGCGAHLGSLRRLETGGYVASQCVGLPALEAMSEQQREAC
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHH
LLPVQSLVANYPVVTLDADNAGRFLSGLRRRGSPGQWGPDAVLVQVYGSDPAAFLGSAHV
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHCCHHH
MADELIPGRLLSPIEIQDMLAPRPPVISNPAASAATA
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA