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The map label for this gene is cysB [H]

Identifier: 91788269

GI number: 91788269

Start: 2502708

End: 2503652

Strand: Reverse

Name: cysB [H]

Synonym: Bpro_2404

Alternate gene names: 91788269

Gene position: 2503652-2502708 (Counterclockwise)

Preceding gene: 91788270

Following gene: 91788268

Centisome position: 48.14

GC content: 60.0

Gene sequence:

>945_bases
ATGAACCTTCACCAATTCCGATTCGTTCAAGAGGCCATACGGCGCAACCTGAACCTCACCGAAACCGCTCGCGCCCTGCA
CACGTCGCAGCCCGGGGTGTCCAAGGCCATCATCGAACTCGAAGAAGAACTCGGGGTGGAGATTTTTGCGCGCCATGGCA
AGCGGCTCAAACGCGTGACCGAACCCGGCGAGCATGTGCTCAAAAGCATAGAACTCATCATGCGCGAGGTGGGTAACCTG
CGGCGTATCGGTGAGCAGTTTTCGGCGCAGGACAGTGGAACGCTGTCGATCGCCACCACGCACACCCAGGCCCGCTACGT
GCTGCCGCAACCGGTGGCGAAGCTGCGCGAAGCTTTCCCCAAGGTCAACGTCAGCCTGCACCAGGGCTCACCCGACCAGG
TGGCCCGCATGGTCATCGACGAGGTCGCCGAAATCGGCATCGCCACCGAATCACTGACCCAGTACGAAGAACTGGTGACC
CTGCCTTGCTACGAATGGCAGCACATGCTCGTGATGCCTGTCGACCATCCGCTGGCCAAAAAGGATGACATCACGCTGGA
GGATCTGGCGCAGGAACCGCTGATCACCTACCACCCATCCTTTACCGGGCGCACCAAGGTTGACCACGCGTTTGCGGCCA
AACGCCTGCAACCGCGCATTGCCCTGGAAGCCATTGACTCTGATGTGATCAAGACCTATGTGCGCCTGGGGCTGGGCGTG
GGGATTGTGGCCGAGATGGCACTCATGGATGACGGCACCAACAGCGACCTCGTGGCCAAGCCGGCCGGCATGTTGTTTGG
CATGAACGTGGCCCGCGTCGCTTTCAAGCGCAGCGCCTACCTGCGCAATTTCGTTTATAAATTTGCCGAGCTGCTCAGCG
ACAAGCTGGACCGCGCCCTTATCACCAAGGCCATGGCAGGCCGCATCAACGACTATGAGATTTGA

Upstream 100 bases:

>100_bases
TGTCCTGACCTTCTTCAGGCGACGAAAAATCAGCGCGTGCAACCATCAATTCATTAGTACAAACGGGCATAATAAAGTCA
ATAATTAGCCTGAATTTGAT

Downstream 100 bases:

>100_bases
TCCACCCCCGAACCGACCTGCGGCCGTTTCCCCCTCAAGGGGGCGCCGCTAGCGGCCCGGCGAAGCCGGTTCCGCGGCGG
CCGCTTGAAAAAACCCTCAC

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNLHQFRFVQEAIRRNLNLTETARALHTSQPGVSKAIIELEEELGVEIFARHGKRLKRVTEPGEHVLKSIELIMREVGNL
RRIGEQFSAQDSGTLSIATTHTQARYVLPQPVAKLREAFPKVNVSLHQGSPDQVARMVIDEVAEIGIATESLTQYEELVT
LPCYEWQHMLVMPVDHPLAKKDDITLEDLAQEPLITYHPSFTGRTKVDHAFAAKRLQPRIALEAIDSDVIKTYVRLGLGV
GIVAEMALMDDGTNSDLVAKPAGMLFGMNVARVAFKRSAYLRNFVYKFAELLSDKLDRALITKAMAGRINDYEI

Sequences:

>Translated_314_residues
MNLHQFRFVQEAIRRNLNLTETARALHTSQPGVSKAIIELEEELGVEIFARHGKRLKRVTEPGEHVLKSIELIMREVGNL
RRIGEQFSAQDSGTLSIATTHTQARYVLPQPVAKLREAFPKVNVSLHQGSPDQVARMVIDEVAEIGIATESLTQYEELVT
LPCYEWQHMLVMPVDHPLAKKDDITLEDLAQEPLITYHPSFTGRTKVDHAFAAKRLQPRIALEAIDSDVIKTYVRLGLGV
GIVAEMALMDDGTNSDLVAKPAGMLFGMNVARVAFKRSAYLRNFVYKFAELLSDKLDRALITKAMAGRINDYEI
>Mature_314_residues
MNLHQFRFVQEAIRRNLNLTETARALHTSQPGVSKAIIELEEELGVEIFARHGKRLKRVTEPGEHVLKSIELIMREVGNL
RRIGEQFSAQDSGTLSIATTHTQARYVLPQPVAKLREAFPKVNVSLHQGSPDQVARMVIDEVAEIGIATESLTQYEELVT
LPCYEWQHMLVMPVDHPLAKKDDITLEDLAQEPLITYHPSFTGRTKVDHAFAAKRLQPRIALEAIDSDVIKTYVRLGLGV
GIVAEMALMDDGTNSDLVAKPAGMLFGMNVARVAFKRSAYLRNFVYKFAELLSDKLDRALITKAMAGRINDYEI

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=311, Percent_Identity=45.016077170418, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1788296, Length=296, Percent_Identity=46.9594594594595, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI157672245, Length=251, Percent_Identity=29.0836653386454, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=28.5223367697594, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=21.8855218855219, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789204, Length=251, Percent_Identity=26.6932270916335, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788887, Length=265, Percent_Identity=26.0377358490566, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=237, Percent_Identity=25.7383966244726, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787879, Length=242, Percent_Identity=26.0330578512397, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367136, Length=239, Percent_Identity=24.6861924686192, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35228; Mature: 35228

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVQEAIRRNLNLTETARALHTSQPGVSKAIIELEEELGVEIFARHGKRLKRVT
CCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHC
EPGEHVLKSIELIMREVGNLRRIGEQFSAQDSGTLSIATTHTQARYVLPQPVAKLREAFP
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCHHHHHHHHHCC
KVNVSLHQGSPDQVARMVIDEVAEIGIATESLTQYEELVTLPCYEWQHMLVMPVDHPLAK
CCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
KDDITLEDLAQEPLITYHPSFTGRTKVDHAFAAKRLQPRIALEAIDSDVIKTYVRLGLGV
CCCCCHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCH
GIVAEMALMDDGTNSDLVAKPAGMLFGMNVARVAFKRSAYLRNFVYKFAELLSDKLDRAL
HHHHHHHHHCCCCCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITKAMAGRINDYEI
HHHHHHCCCCCCCC
>Mature Secondary Structure
MNLHQFRFVQEAIRRNLNLTETARALHTSQPGVSKAIIELEEELGVEIFARHGKRLKRVT
CCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHC
EPGEHVLKSIELIMREVGNLRRIGEQFSAQDSGTLSIATTHTQARYVLPQPVAKLREAFP
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCHHHHHHHHHCC
KVNVSLHQGSPDQVARMVIDEVAEIGIATESLTQYEELVTLPCYEWQHMLVMPVDHPLAK
CCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
KDDITLEDLAQEPLITYHPSFTGRTKVDHAFAAKRLQPRIALEAIDSDVIKTYVRLGLGV
CCCCCHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCH
GIVAEMALMDDGTNSDLVAKPAGMLFGMNVARVAFKRSAYLRNFVYKFAELLSDKLDRAL
HHHHHHHHHCCCCCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITKAMAGRINDYEI
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]