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The map label for this gene is nemA [H]

Identifier: 91788215

GI number: 91788215

Start: 2445499

End: 2446575

Strand: Reverse

Name: nemA [H]

Synonym: Bpro_2348

Alternate gene names: 91788215

Gene position: 2446575-2445499 (Counterclockwise)

Preceding gene: 91788216

Following gene: 91788213

Centisome position: 47.05

GC content: 64.53

Gene sequence:

>1077_bases
ATGCTTTTCGAAGCCTTTCCCCTGCGTGCCACCCAACTCAGCAATCGCACTGTGATGTCGCCGCTCACGCGCAGCCGCGC
GAGCGAGAACAACACACCCAACAGCCTGATGGCCACCTACTATGCCCAGCGCGCGAGCGCCGGCCTCATCATCACGGAAG
GAACCTCGCCGTCGCCCAACGGCTTGGGCTATGCCCGCATCCCCGGCCTGTTCAATGACGCCCAGGTGCAAGGCTGGAAG
CTTGTAACTGACGCAGTCCATGCCAAAGGCGGCAAGATCGTCGTGCAGCTGATGCATACCGGCCGCGTGACCCACCAGGC
CAACCTGCCGGCCGGCGCCGAAGTGCTTGGCCCCTCACCCGAGACCTGCCCTGGTGAGATGTGGACTGATGCACAAGGCA
GCCAGCCGCACACACCGCCGCGCGCCATGACCGAGGCCGACATCCAAGCCGTGATCGGCGAGTACACAACGGCCGCGCGC
CTTGCCATCCAAGCCGGCTTTGACGGCATCGAGCTGCACGCGGCCAACGGTTACTTGCTTGAGCAATTCCTGAACGCCAA
CGTCAATCGCCGCACTGACAGCTACGGCGGCACGGCCGAAGGGCGCAACCGGCTGGTGCTCGAGGTCGCCCGTGCTGCCG
TGAAGGAGATAGGCGCTGAGCGAGTCGGCATCCGCCTGTCGCCTCACGGCGTGGTCAATAGCACCGGCGCGTTCGAAGGC
GTGGACGAACAGTACCTGGCGCTGGTGAAGGAACTCTCCGCACTCGGTCTCATGTACGTTCACGTCCTGGACCATTCGGC
GATGGGCGCCCCGCCCGTCCCGGCAAAGCTGAAGTCCGATTTGAGGGCTGCGTTCGACGGCCCCTTCATCCTCGCCGGCG
GACAGAATAAAGCCAGCGCACAACAGGCGTTGAGCGAAGGCCGCGCGGACCTGATCGCCATAGGCAGGCCCTTCATCGCC
AACCCTGACCTGGTCGAGCGCATGCGCCAGGACGCGCCGTTGATCGCTCCTGTCCCGGACACGTTCTATACCCCCGGCGC
CAAGGGCTACACAGACTATCCGATGTTCGCAGGTTGA

Upstream 100 bases:

>100_bases
GTCCAGCTAACGGAACGTTACGGCTCTTCCTCTTCTCCCCATTTTCCATTTTCCATTTTCCATTTTCCATTTTCCATTTT
CCATTTTCTCAAGGACAGAC

Downstream 100 bases:

>100_bases
GCATCAAACGTCCCCGATTGGCCCCCAACTCGAACACGCAAACCTCGCAGGCCTTGGAGTTCCTTGGCCGCTCCACACGA
TAGGGAGAGCGATTTTGAGT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLFEAFPLRATQLSNRTVMSPLTRSRASENNTPNSLMATYYAQRASAGLIITEGTSPSPNGLGYARIPGLFNDAQVQGWK
LVTDAVHAKGGKIVVQLMHTGRVTHQANLPAGAEVLGPSPETCPGEMWTDAQGSQPHTPPRAMTEADIQAVIGEYTTAAR
LAIQAGFDGIELHAANGYLLEQFLNANVNRRTDSYGGTAEGRNRLVLEVARAAVKEIGAERVGIRLSPHGVVNSTGAFEG
VDEQYLALVKELSALGLMYVHVLDHSAMGAPPVPAKLKSDLRAAFDGPFILAGGQNKASAQQALSEGRADLIAIGRPFIA
NPDLVERMRQDAPLIAPVPDTFYTPGAKGYTDYPMFAG

Sequences:

>Translated_358_residues
MLFEAFPLRATQLSNRTVMSPLTRSRASENNTPNSLMATYYAQRASAGLIITEGTSPSPNGLGYARIPGLFNDAQVQGWK
LVTDAVHAKGGKIVVQLMHTGRVTHQANLPAGAEVLGPSPETCPGEMWTDAQGSQPHTPPRAMTEADIQAVIGEYTTAAR
LAIQAGFDGIELHAANGYLLEQFLNANVNRRTDSYGGTAEGRNRLVLEVARAAVKEIGAERVGIRLSPHGVVNSTGAFEG
VDEQYLALVKELSALGLMYVHVLDHSAMGAPPVPAKLKSDLRAAFDGPFILAGGQNKASAQQALSEGRADLIAIGRPFIA
NPDLVERMRQDAPLIAPVPDTFYTPGAKGYTDYPMFAG
>Mature_358_residues
MLFEAFPLRATQLSNRTVMSPLTRSRASENNTPNSLMATYYAQRASAGLIITEGTSPSPNGLGYARIPGLFNDAQVQGWK
LVTDAVHAKGGKIVVQLMHTGRVTHQANLPAGAEVLGPSPETCPGEMWTDAQGSQPHTPPRAMTEADIQAVIGEYTTAAR
LAIQAGFDGIELHAANGYLLEQFLNANVNRRTDSYGGTAEGRNRLVLEVARAAVKEIGAERVGIRLSPHGVVNSTGAFEG
VDEQYLALVKELSALGLMYVHVLDHSAMGAPPVPAKLKSDLRAAFDGPFILAGGQNKASAQQALSEGRADLIAIGRPFIA
NPDLVERMRQDAPLIAPVPDTFYTPGAKGYTDYPMFAG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=40.2777777777778, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1789463, Length=337, Percent_Identity=29.673590504451, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17559804, Length=362, Percent_Identity=26.5193370165746, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=326, Percent_Identity=27.9141104294479, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=316, Percent_Identity=26.8987341772152, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=302, Percent_Identity=27.8145695364238, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=362, Percent_Identity=24.8618784530387, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=254, Percent_Identity=30.7086614173228, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=39.5778364116095, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321973, Length=375, Percent_Identity=37.0666666666667, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38103; Mature: 38103

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFEAFPLRATQLSNRTVMSPLTRSRASENNTPNSLMATYYAQRASAGLIITEGTSPSPN
CCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
GLGYARIPGLFNDAQVQGWKLVTDAVHAKGGKIVVQLMHTGRVTHQANLPAGAEVLGPSP
CCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCC
ETCPGEMWTDAQGSQPHTPPRAMTEADIQAVIGEYTTAARLAIQAGFDGIELHAANGYLL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
EQFLNANVNRRTDSYGGTAEGRNRLVLEVARAAVKEIGAERVGIRLSPHGVVNSTGAFEG
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCCCC
VDEQYLALVKELSALGLMYVHVLDHSAMGAPPVPAKLKSDLRAAFDGPFILAGGQNKASA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHH
QQALSEGRADLIAIGRPFIANPDLVERMRQDAPLIAPVPDTFYTPGAKGYTDYPMFAG
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLFEAFPLRATQLSNRTVMSPLTRSRASENNTPNSLMATYYAQRASAGLIITEGTSPSPN
CCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
GLGYARIPGLFNDAQVQGWKLVTDAVHAKGGKIVVQLMHTGRVTHQANLPAGAEVLGPSP
CCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCC
ETCPGEMWTDAQGSQPHTPPRAMTEADIQAVIGEYTTAARLAIQAGFDGIELHAANGYLL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
EQFLNANVNRRTDSYGGTAEGRNRLVLEVARAAVKEIGAERVGIRLSPHGVVNSTGAFEG
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCCCC
VDEQYLALVKELSALGLMYVHVLDHSAMGAPPVPAKLKSDLRAAFDGPFILAGGQNKASA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHH
QQALSEGRADLIAIGRPFIANPDLVERMRQDAPLIAPVPDTFYTPGAKGYTDYPMFAG
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]