| Definition | Polaromonas sp. JS666 chromosome, complete genome. |
|---|---|
| Accession | NC_007948 |
| Length | 5,200,264 |
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The map label for this gene is fabG [C]
Identifier: 91788158
GI number: 91788158
Start: 2381888
End: 2382649
Strand: Reverse
Name: fabG [C]
Synonym: Bpro_2289
Alternate gene names: 91788158
Gene position: 2382649-2381888 (Counterclockwise)
Preceding gene: 91788159
Following gene: 91788157
Centisome position: 45.82
GC content: 61.29
Gene sequence:
>762_bases ATGAGGGTGAAAGACAAATCCATCATCGTCACCGGCTCTGGCGGCGGTATCGGCGAAGGCATTGCCAGACGGCTGGCCGC CGAGGGCGCCAAGGTCATCGTCAATGACATCAACGTGGCCATGGGTGAAAAAGTCGTGGCAGCCATCACCAAGGCCGGTG GCACCGCTTCATTTTTTGCCGCCGATGTCACGAAATCCGCAGAAGTCAAGGCGCTGGTCGATGCGGCCATGCAGCGTTAC GGCAAGCTCGACGTGATGGTCAACAACGCAGGCTGGACGCACCGCAACCAGCCCGCGCTTGACGTGAGTGAAGATGACTT CGACAAGTGCTACGCCATCAATGTCAAAAGCATTTACCTGGCCACCATTCATGCAACGCCGGTGTTTCGCCGCCAGGGTG GCGGCAGCTTCATCAATATCGCCTCCACGGCAGGTGTGCGCCCGCGTCCGGGCCTGACCTGGTACAACGGCTCCAAAGGC GCGGTCATCACCACCTCGAAATCCCTGGCGGCGGAACTCGGGCCCGACAACATCCGGGTGAATTGCATCAACCCGGTGTT CAACCCCGATACCGGCCTGTCGGCCGAATTTGCCGGGGGGCCACTGGATGAAGAACGCCGCGAGCGGTTTCGCGCCAGCA TCCCGCTGGGCCGTTTTTCCACGGCGCTGGATGTGGCCAATGCGGCCCTTTACCTGGCCAGCGACGAGGCCGATTTCATC AGTGGCGTCTGCATCGAGGTGGATGGCGCGCGCTGTGTATAG
Upstream 100 bases:
>100_bases CACCACACTGAAGACCGTGGCGGTGCGGCATGGCTGACAGGCTCCCCGGCCCGCCAGATGTACTAAGCTTACGTATCAAT AAAAACCTGGAGACAACCCA
Downstream 100 bases:
>100_bases AGCCCCCACGCTCGTATAAAGCCCCCACGCTCCCCACTGCGTGTGGTTCGCTGCCCCCCGAGGGGGCGCTGCGCCTGCGG CCCGGCTAAGCCGGTTCCGC
Product: 3-ketoacyl-(acyl-carrier-protein) reductase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: NA
Number of amino acids: Translated: 253; Mature: 253
Protein sequence:
>253_residues MRVKDKSIIVTGSGGGIGEGIARRLAAEGAKVIVNDINVAMGEKVVAAITKAGGTASFFAADVTKSAEVKALVDAAMQRY GKLDVMVNNAGWTHRNQPALDVSEDDFDKCYAINVKSIYLATIHATPVFRRQGGGSFINIASTAGVRPRPGLTWYNGSKG AVITTSKSLAAELGPDNIRVNCINPVFNPDTGLSAEFAGGPLDEERRERFRASIPLGRFSTALDVANAALYLASDEADFI SGVCIEVDGARCV
Sequences:
>Translated_253_residues MRVKDKSIIVTGSGGGIGEGIARRLAAEGAKVIVNDINVAMGEKVVAAITKAGGTASFFAADVTKSAEVKALVDAAMQRY GKLDVMVNNAGWTHRNQPALDVSEDDFDKCYAINVKSIYLATIHATPVFRRQGGGSFINIASTAGVRPRPGLTWYNGSKG AVITTSKSLAAELGPDNIRVNCINPVFNPDTGLSAEFAGGPLDEERRERFRASIPLGRFSTALDVANAALYLASDEADFI SGVCIEVDGARCV >Mature_253_residues MRVKDKSIIVTGSGGGIGEGIARRLAAEGAKVIVNDINVAMGEKVVAAITKAGGTASFFAADVTKSAEVKALVDAAMQRY GKLDVMVNNAGWTHRNQPALDVSEDDFDKCYAINVKSIYLATIHATPVFRRQGGGSFINIASTAGVRPRPGLTWYNGSKG AVITTSKSLAAELGPDNIRVNCINPVFNPDTGLSAEFAGGPLDEERRERFRASIPLGRFSTALDVANAALYLASDEADFI SGVCIEVDGARCV
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=245, Percent_Identity=34.2857142857143, Blast_Score=133, Evalue=2e-31, Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=31.9066147859922, Blast_Score=110, Evalue=1e-24, Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=36.2637362637363, Blast_Score=110, Evalue=1e-24, Organism=Homo sapiens, GI126723750, Length=250, Percent_Identity=30.4, Blast_Score=110, Evalue=2e-24, Organism=Homo sapiens, GI66933014, Length=251, Percent_Identity=35.8565737051793, Blast_Score=109, Evalue=3e-24, Organism=Homo sapiens, GI5031737, Length=241, Percent_Identity=32.3651452282158, Blast_Score=105, Evalue=3e-23, Organism=Homo sapiens, GI59889578, Length=230, Percent_Identity=34.3478260869565, Blast_Score=103, Evalue=2e-22, Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=29.3877551020408, Blast_Score=99, Evalue=4e-21, Organism=Homo sapiens, GI7705925, Length=245, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=6e-21, Organism=Homo sapiens, GI33667109, Length=181, Percent_Identity=33.7016574585635, Blast_Score=94, Evalue=2e-19, Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=29.3650793650794, Blast_Score=88, Evalue=6e-18, Organism=Homo sapiens, GI302344755, Length=109, Percent_Identity=39.4495412844037, Blast_Score=84, Evalue=9e-17, Organism=Homo sapiens, GI31542939, Length=194, Percent_Identity=30.9278350515464, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI4504505, Length=194, Percent_Identity=30.4123711340206, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI210032110, Length=198, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=4e-14, Organism=Homo sapiens, GI109715829, Length=249, Percent_Identity=27.710843373494, Blast_Score=75, Evalue=7e-14, Organism=Homo sapiens, GI4758504, Length=264, Percent_Identity=29.9242424242424, Blast_Score=72, Evalue=5e-13, Organism=Homo sapiens, GI19923817, Length=253, Percent_Identity=25.296442687747, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI224922801, Length=175, Percent_Identity=29.1428571428571, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI142976729, Length=197, Percent_Identity=26.9035532994924, Blast_Score=70, Evalue=2e-12, Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=34.136546184739, Blast_Score=124, Evalue=6e-30, Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=32.258064516129, Blast_Score=118, Evalue=3e-28, Organism=Escherichia coli, GI87082100, Length=252, Percent_Identity=34.1269841269841, Blast_Score=115, Evalue=3e-27, Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=30.5220883534137, Blast_Score=110, Evalue=8e-26, Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=31.2, Blast_Score=108, Evalue=4e-25, Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=31.4516129032258, Blast_Score=108, Evalue=5e-25, Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=31.1023622047244, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=28.6852589641434, Blast_Score=100, Evalue=1e-22, Organism=Escherichia coli, GI87082160, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=3e-22, Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=29.7959183673469, Blast_Score=94, Evalue=1e-20, Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=32.5301204819277, Blast_Score=91, Evalue=5e-20, Organism=Escherichia coli, GI1787820, Length=178, Percent_Identity=29.2134831460674, Blast_Score=70, Evalue=2e-13, Organism=Escherichia coli, GI1789057, Length=257, Percent_Identity=26.8482490272374, Blast_Score=68, Evalue=5e-13, Organism=Escherichia coli, GI1788891, Length=261, Percent_Identity=28.735632183908, Blast_Score=64, Evalue=1e-11, Organism=Escherichia coli, GI1787526, Length=252, Percent_Identity=25.3968253968254, Blast_Score=61, Evalue=8e-11, Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=32.9317269076305, Blast_Score=120, Evalue=9e-28, Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=34.6774193548387, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=31.9838056680162, Blast_Score=110, Evalue=5e-25, Organism=Caenorhabditis elegans, GI71994604, Length=246, Percent_Identity=31.7073170731707, Blast_Score=107, Evalue=6e-24, Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=30.859375, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=32.319391634981, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=30.6772908366534, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17568967, Length=194, Percent_Identity=35.5670103092784, Blast_Score=97, Evalue=7e-21, Organism=Caenorhabditis elegans, GI193204405, Length=261, Percent_Identity=29.8850574712644, Blast_Score=97, Evalue=8e-21, Organism=Caenorhabditis elegans, GI17562906, Length=263, Percent_Identity=30.7984790874525, Blast_Score=95, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=30.6451612903226, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17544670, Length=262, Percent_Identity=29.3893129770992, Blast_Score=94, Evalue=7e-20, Organism=Caenorhabditis elegans, GI17562910, Length=259, Percent_Identity=30.1158301158301, Blast_Score=94, Evalue=9e-20, Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=30.5019305019305, Blast_Score=91, Evalue=5e-19, Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=29.1187739463602, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17538480, Length=263, Percent_Identity=30.0380228136882, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17564282, Length=194, Percent_Identity=28.8659793814433, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17565030, Length=262, Percent_Identity=30.1526717557252, Blast_Score=87, Evalue=8e-18, Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=29.8850574712644, Blast_Score=87, Evalue=9e-18, Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=29.0566037735849, Blast_Score=86, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17562904, Length=260, Percent_Identity=28.4615384615385, Blast_Score=84, Evalue=9e-17, Organism=Caenorhabditis elegans, GI17508895, Length=168, Percent_Identity=29.1666666666667, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17508651, Length=237, Percent_Identity=27.4261603375527, Blast_Score=82, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=27.4809160305344, Blast_Score=80, Evalue=9e-16, Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=28.3018867924528, Blast_Score=80, Evalue=9e-16, Organism=Caenorhabditis elegans, GI17562990, Length=262, Percent_Identity=27.0992366412214, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI193203117, Length=189, Percent_Identity=25.3968253968254, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI193203115, Length=187, Percent_Identity=25.668449197861, Blast_Score=77, Evalue=8e-15, Organism=Caenorhabditis elegans, GI72000259, Length=260, Percent_Identity=27.6923076923077, Blast_Score=77, Evalue=9e-15, Organism=Caenorhabditis elegans, GI17560220, Length=260, Percent_Identity=28.0769230769231, Blast_Score=77, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17536651, Length=251, Percent_Identity=27.4900398406374, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17536025, Length=256, Percent_Identity=28.125, Blast_Score=72, Evalue=4e-13, Organism=Caenorhabditis elegans, GI17538182, Length=188, Percent_Identity=31.3829787234043, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=24.9056603773585, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17561272, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=6e-12, Organism=Caenorhabditis elegans, GI71982365, Length=194, Percent_Identity=25.7731958762887, Blast_Score=64, Evalue=1e-10, Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=32.3076923076923, Blast_Score=91, Evalue=1e-19, Organism=Saccharomyces cerevisiae, GI6323882, Length=238, Percent_Identity=26.0504201680672, Blast_Score=71, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6322227, Length=190, Percent_Identity=27.3684210526316, Blast_Score=66, Evalue=5e-12, Organism=Saccharomyces cerevisiae, GI6324126, Length=188, Percent_Identity=26.5957446808511, Blast_Score=62, Evalue=6e-11, Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=37.007874015748, Blast_Score=139, Evalue=2e-33, Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=35.1778656126482, Blast_Score=134, Evalue=8e-32, Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=33.984375, Blast_Score=132, Evalue=2e-31, Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=34.251968503937, Blast_Score=126, Evalue=1e-29, Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=30.9236947791165, Blast_Score=117, Evalue=8e-27, Organism=Drosophila melanogaster, GI24643142, Length=254, Percent_Identity=31.8897637795276, Blast_Score=108, Evalue=5e-24, Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=28.8617886178862, Blast_Score=99, Evalue=2e-21, Organism=Drosophila melanogaster, GI24644337, Length=214, Percent_Identity=34.5794392523364, Blast_Score=95, Evalue=6e-20, Organism=Drosophila melanogaster, GI24640492, Length=233, Percent_Identity=29.1845493562232, Blast_Score=83, Evalue=2e-16, Organism=Drosophila melanogaster, GI18859837, Length=194, Percent_Identity=29.3814432989691, Blast_Score=80, Evalue=9e-16, Organism=Drosophila melanogaster, GI24584748, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=2e-15, Organism=Drosophila melanogaster, GI161076933, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=2e-15, Organism=Drosophila melanogaster, GI28574402, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=4e-14, Organism=Drosophila melanogaster, GI24584744, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=5e-14, Organism=Drosophila melanogaster, GI17737361, Length=267, Percent_Identity=29.5880149812734, Blast_Score=75, Evalue=6e-14, Organism=Drosophila melanogaster, GI281364892, Length=197, Percent_Identity=30.9644670050761, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI24649181, Length=184, Percent_Identity=29.8913043478261, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI21357673, Length=195, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI221510726, Length=188, Percent_Identity=30.8510638297872, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI21355631, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26639; Mature: 26639
Theoretical pI: Translated: 7.29; Mature: 7.29
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRVKDKSIIVTGSGGGIGEGIARRLAAEGAKVIVNDINVAMGEKVVAAITKAGGTASFFA CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCEEEHHHCCCCCEEEEE ADVTKSAEVKALVDAAMQRYGKLDVMVNNAGWTHRNQPALDVSEDDFDKCYAINVKSIYL ECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEE ATIHATPVFRRQGGGSFINIASTAGVRPRPGLTWYNGSKGAVITTSKSLAAELGPDNIRV EEEECCEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEECHHHHHHCCCCCEEE NCINPVFNPDTGLSAEFAGGPLDEERRERFRASIPLGRFSTALDVANAALYLASDEADFI EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCHHH SGVCIEVDGARCV CEEEEEECCCCCC >Mature Secondary Structure MRVKDKSIIVTGSGGGIGEGIARRLAAEGAKVIVNDINVAMGEKVVAAITKAGGTASFFA CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCEEEHHHCCCCCEEEEE ADVTKSAEVKALVDAAMQRYGKLDVMVNNAGWTHRNQPALDVSEDDFDKCYAINVKSIYL ECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEE ATIHATPVFRRQGGGSFINIASTAGVRPRPGLTWYNGSKGAVITTSKSLAAELGPDNIRV EEEECCEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEECHHHHHHCCCCCEEE NCINPVFNPDTGLSAEFAGGPLDEERRERFRASIPLGRFSTALDVANAALYLASDEADFI EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCHHH SGVCIEVDGARCV CEEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 10360571 [H]