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The map label for this gene is phaZ1 [H]

Identifier: 91787941

GI number: 91787941

Start: 2146073

End: 2147152

Strand: Reverse

Name: phaZ1 [H]

Synonym: Bpro_2065

Alternate gene names: 91787941

Gene position: 2147152-2146073 (Counterclockwise)

Preceding gene: 91787942

Following gene: 91787934

Centisome position: 41.29

GC content: 69.63

Gene sequence:

>1080_bases
ATGACCCCTGATTTCCAACGCCTGATGAAGGATGCCGCCCAGCTCACCCGTGCCGGTGACCTGCAAGCCGCCACCGCCGC
GATCCAGGCCGCGCTGGGCGGCGGTGTGGCTGCCGCAGCCGCCGCCGCACAAGACCTGCGCGACGTGATTGATGTTGCGG
CGCGCGAGGTGCCTGACGTTGCGCGGACATTTGACGACGAGTCCCCCTCCGCACCGGCCGGCAACGCTCCCGACGCCGGC
CGCTTCATTGCCGGCAGCCATGGCGACGCTTCGGGTCACCGCGACTTCAAACTTTATATTCCGCCGGGCGCCGGCGAGCG
CCCGCTGCCGCTGGTTGTGATGCTGCACGGCTGCACGCAGAACCCGGACGATTTTGCGGCGGGCACCGCCATGAACGATG
CGGCGCTGGCGCAGGGCTTTTTTGTGCTGTACCCGGCACAGTCGCAGCAGATGAATCCGCAGCGTTGCTGGAACTGGTTC
AAACACACCCACCAGGCCCGCGGACGCGGCGAGCCGGCGCTGCTGGCGGGCATGACGCGCGAGGTGATGGCGCGCCATGC
GATTGACCCGAGGCGCGTTTATGTGGCCGGCCTGTCCGCGGGTGGCGCCATGGCCGCCATTTTGGGCGACGCCTATCCTG
ACCTGTATGCCGCGGTGGGCGTGCACTCGGGGCTGGCGGCCGGCATCGCGACAGACCTGCCGTCGGCGCTGGGCGCGATG
AGGGGCGGTGGCACCACCACCCGTGCGACCGCCAGCGGCGTGGCGACCATCGTCTTTCACGGCGACGCCGACACCACGGT
GCATCCGGGCAACGGCGAGCAGGTCATCACGGCCAGCGTTGGCACGGCGGCCGCTGTCGAAGTCGAACACCTGAAAGGAG
CTGACGGCCGCGCGACCACGCGCCGCGTGCACCGCGCGAGCGATGGCACGGTGGTGGCCGAGCACTGGCTGGTGCACGGC
ACATCGCACGCCTGGTCAGGCGGCAGCGCCAACGGCTCGTACACCGACGCCCGGGGGCCAGATGCCAGCGCCGAGATGGT
GCGGTTTTTTCTGGAGCATCCGCACCGGCAGGCCCACTAA

Upstream 100 bases:

>100_bases
CATGCGAGCCCCGCCGTGAAAGCCGCGCTCGTGCAGCGTATCCGCTAATACAGCGAATACAGCGAGTCAACGAATCAACG
AATCGAACTGAAAGAAGCTG

Downstream 100 bases:

>100_bases
CTCATTAACGGCCCGAGCGCACCGGGTTCAGCACAAAATCATATTCCAGCAGATAGTCGCCATCGGGTTGCCTCACCCAA
TCTGCCACCAGCGACTGGCG

Product: esterase, PHB depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTPDFQRLMKDAAQLTRAGDLQAATAAIQAALGGGVAAAAAAAQDLRDVIDVAAREVPDVARTFDDESPSAPAGNAPDAG
RFIAGSHGDASGHRDFKLYIPPGAGERPLPLVVMLHGCTQNPDDFAAGTAMNDAALAQGFFVLYPAQSQQMNPQRCWNWF
KHTHQARGRGEPALLAGMTREVMARHAIDPRRVYVAGLSAGGAMAAILGDAYPDLYAAVGVHSGLAAGIATDLPSALGAM
RGGGTTTRATASGVATIVFHGDADTTVHPGNGEQVITASVGTAAAVEVEHLKGADGRATTRRVHRASDGTVVAEHWLVHG
TSHAWSGGSANGSYTDARGPDASAEMVRFFLEHPHRQAH

Sequences:

>Translated_359_residues
MTPDFQRLMKDAAQLTRAGDLQAATAAIQAALGGGVAAAAAAAQDLRDVIDVAAREVPDVARTFDDESPSAPAGNAPDAG
RFIAGSHGDASGHRDFKLYIPPGAGERPLPLVVMLHGCTQNPDDFAAGTAMNDAALAQGFFVLYPAQSQQMNPQRCWNWF
KHTHQARGRGEPALLAGMTREVMARHAIDPRRVYVAGLSAGGAMAAILGDAYPDLYAAVGVHSGLAAGIATDLPSALGAM
RGGGTTTRATASGVATIVFHGDADTTVHPGNGEQVITASVGTAAAVEVEHLKGADGRATTRRVHRASDGTVVAEHWLVHG
TSHAWSGGSANGSYTDARGPDASAEMVRFFLEHPHRQAH
>Mature_358_residues
TPDFQRLMKDAAQLTRAGDLQAATAAIQAALGGGVAAAAAAAQDLRDVIDVAAREVPDVARTFDDESPSAPAGNAPDAGR
FIAGSHGDASGHRDFKLYIPPGAGERPLPLVVMLHGCTQNPDDFAAGTAMNDAALAQGFFVLYPAQSQQMNPQRCWNWFK
HTHQARGRGEPALLAGMTREVMARHAIDPRRVYVAGLSAGGAMAAILGDAYPDLYAAVGVHSGLAAGIATDLPSALGAMR
GGGTTTRATASGVATIVFHGDADTTVHPGNGEQVITASVGTAAAVEVEHLKGADGRATTRRVHRASDGTVVAEHWLVHGT
SHAWSGGSANGSYTDARGPDASAEMVRFFLEHPHRQAH

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37156; Mature: 37025

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDFQRLMKDAAQLTRAGDLQAATAAIQAALGGGVAAAAAAAQDLRDVIDVAAREVPDV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH
ARTFDDESPSAPAGNAPDAGRFIAGSHGDASGHRDFKLYIPPGAGERPLPLVVMLHGCTQ
HHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCC
NPDDFAAGTAMNDAALAQGFFVLYPAQSQQMNPQRCWNWFKHTHQARGRGEPALLAGMTR
CCCHHHCCCCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVMARHAIDPRRVYVAGLSAGGAMAAILGDAYPDLYAAVGVHSGLAAGIATDLPSALGAM
HHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGGGTTTRATASGVATIVFHGDADTTVHPGNGEQVITASVGTAAAVEVEHLKGADGRATT
CCCCCCCEEECCCEEEEEEECCCCCEEECCCCCEEEEEECCCEEEEEHHHHCCCCCCHHH
RRVHRASDGTVVAEHWLVHGTSHAWSGGSANGSYTDARGPDASAEMVRFFLEHPHRQAH
HHHHHCCCCCEEEEEHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TPDFQRLMKDAAQLTRAGDLQAATAAIQAALGGGVAAAAAAAQDLRDVIDVAAREVPDV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH
ARTFDDESPSAPAGNAPDAGRFIAGSHGDASGHRDFKLYIPPGAGERPLPLVVMLHGCTQ
HHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCC
NPDDFAAGTAMNDAALAQGFFVLYPAQSQQMNPQRCWNWFKHTHQARGRGEPALLAGMTR
CCCHHHCCCCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVMARHAIDPRRVYVAGLSAGGAMAAILGDAYPDLYAAVGVHSGLAAGIATDLPSALGAM
HHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGGGTTTRATASGVATIVFHGDADTTVHPGNGEQVITASVGTAAAVEVEHLKGADGRATT
CCCCCCCEEECCCEEEEEEECCCCCEEECCCCCEEEEEECCCEEEEEHHHHCCCCCCHHH
RRVHRASDGTVVAEHWLVHGTSHAWSGGSANGSYTDARGPDASAEMVRFFLEHPHRQAH
HHHHHCCCCCEEEEEHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]