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The map label for this gene is gloB

Identifier: 91787931

GI number: 91787931

Start: 2136254

End: 2137045

Strand: Reverse

Name: gloB

Synonym: Bpro_2055

Alternate gene names: 91787931

Gene position: 2137045-2136254 (Counterclockwise)

Preceding gene: 91787932

Following gene: 91787930

Centisome position: 41.09

GC content: 65.4

Gene sequence:

>792_bases
ATGATCGTCGAACGCATCTGGACCGGTAATGCCTACCGCAATTTCAATTACCTGATCGCCTGCCCCGAGACCGGCGAAGC
GCTGGCCATCGACCCGCTCGACCATGAAAAGACGCTGGCCACCGCCAGGGTCAAGGGCTGGCAGATCACGCAGGTCCTCA
ACACCCACGAGCACCACGACCACACCGGCGGCAACGCGGCCGTGATTGCGGCCACCGGCGCCAAACTGATCGCCCACCAC
AAGGCCGGCGGGCGCATTGCGGGTGTGGACCGGGGCGTGAAGGCCGGCGATGTGATCAAGGTCGGCAAGACCGTCGAGCT
GGAGTGCCTGGACACGCCCGGCCACACGATGTGCCACATCTGCCTGCGCTCGCACACCGAGCAGCCGGCGCTGTTTTCGG
GAGACACGCTGTTCAATGCGGGCGCCGGCAATGTGCACAACGGCGGCGACGTCAACGCGCTGTATGCCACCTTTGCGGAA
CAGCTGGCCCGGCTGCCGGACGACACCCTGGTCTATCCGGGCCACGACTACATCGAGAACAACCTCCGGTTCACCCTGGC
GCGCGAACCCGACAACGCCGCCGCAAAAGCCCTGCTGCCCAGCGTGACCGACCATGACCCCGCGAAGTCGGCCGTCACCA
CGCTCAAGGACGAAAAAGAGTTCAACACCTTCCTGCGCCTGAGCAGCCCCAGCGTCATCGCCAGGCTGCGCGAGAGCTTT
CCCGACCTGCCGGACGAGCCCGACGCCAGAACGGTGTTTGCCAAGCTGCGGGAACTGCGCAACCAGTGGTGA

Upstream 100 bases:

>100_bases
TGCCCCGGACTTGATCCGGGGTCCGGGATGACAAACCAGGAGGCAAGGGCTTAACTGACTAGTATTGCCGCTCACCGTTA
ACAAGCCCCTGGAGCCCGCT

Downstream 100 bases:

>100_bases
GCGGAGAGTGACAGACACGAGCCGCAACATGCAGCAGGACCAGCATCTCAGAGCACGCATCGAGGAGATGCTGCGCGCCT
TCACGCTGCAGGCAGCCGAG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MIVERIWTGNAYRNFNYLIACPETGEALAIDPLDHEKTLATARVKGWQITQVLNTHEHHDHTGGNAAVIAATGAKLIAHH
KAGGRIAGVDRGVKAGDVIKVGKTVELECLDTPGHTMCHICLRSHTEQPALFSGDTLFNAGAGNVHNGGDVNALYATFAE
QLARLPDDTLVYPGHDYIENNLRFTLAREPDNAAAKALLPSVTDHDPAKSAVTTLKDEKEFNTFLRLSSPSVIARLRESF
PDLPDEPDARTVFAKLRELRNQW

Sequences:

>Translated_263_residues
MIVERIWTGNAYRNFNYLIACPETGEALAIDPLDHEKTLATARVKGWQITQVLNTHEHHDHTGGNAAVIAATGAKLIAHH
KAGGRIAGVDRGVKAGDVIKVGKTVELECLDTPGHTMCHICLRSHTEQPALFSGDTLFNAGAGNVHNGGDVNALYATFAE
QLARLPDDTLVYPGHDYIENNLRFTLAREPDNAAAKALLPSVTDHDPAKSAVTTLKDEKEFNTFLRLSSPSVIARLRESF
PDLPDEPDARTVFAKLRELRNQW
>Mature_263_residues
MIVERIWTGNAYRNFNYLIACPETGEALAIDPLDHEKTLATARVKGWQITQVLNTHEHHDHTGGNAAVIAATGAKLIAHH
KAGGRIAGVDRGVKAGDVIKVGKTVELECLDTPGHTMCHICLRSHTEQPALFSGDTLFNAGAGNVHNGGDVNALYATFAE
QLARLPDDTLVYPGHDYIENNLRFTLAREPDNAAAKALLPSVTDHDPAKSAVTTLKDEKEFNTFLRLSSPSVIARLRESF
PDLPDEPDARTVFAKLRELRNQW

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=237, Percent_Identity=35.4430379746835, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI94538322, Length=235, Percent_Identity=35.7446808510638, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI14150041, Length=228, Percent_Identity=33.7719298245614, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI21703352, Length=219, Percent_Identity=31.5068493150685, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI116642887, Length=219, Percent_Identity=31.5068493150685, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI46361987, Length=193, Percent_Identity=34.7150259067358, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI41327741, Length=170, Percent_Identity=31.1764705882353, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1786406, Length=244, Percent_Identity=32.3770491803279, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787158, Length=191, Percent_Identity=31.413612565445, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=263, Percent_Identity=33.4600760456274, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17538952, Length=167, Percent_Identity=31.7365269461078, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320478, Length=267, Percent_Identity=28.4644194756554, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=270, Percent_Identity=25.5555555555556, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356335, Length=229, Percent_Identity=32.3144104803493, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24667703, Length=229, Percent_Identity=32.3144104803493, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24667711, Length=229, Percent_Identity=32.3144104803493, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI221330176, Length=169, Percent_Identity=31.9526627218935, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_POLSJ (Q12BV7)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548883.1
- ProteinModelPortal:   Q12BV7
- SMR:   Q12BV7
- STRING:   Q12BV7
- GeneID:   4015281
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2055
- NMPDR:   fig|296591.1.peg.2550
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   LIACPET
- PhylomeDB:   Q12BV7
- ProtClustDB:   CLSK752984
- BioCyc:   PSP296591:BPRO_2055-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28759; Mature: 28759

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVERIWTGNAYRNFNYLIACPETGEALAIDPLDHEKTLATARVKGWQITQVLNTHEHHD
CCEEEEECCCCEECCEEEEECCCCCCEEEECCCCCCHHHHHHHCCCEEEEHHHHCCCCCC
HTGGNAAVIAATGAKLIAHHKAGGRIAGVDRGVKAGDVIKVGKTVELECLDTPGHTMCHI
CCCCCEEEEEECCCEEEEEECCCCEEECCCCCCCCCCEEEECCEEEEEEECCCCHHHHHH
CLRSHTEQPALFSGDTLFNAGAGNVHNGGDVNALYATFAEQLARLPDDTLVYPGHDYIEN
HHHCCCCCCCEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHCC
NLRFTLAREPDNAAAKALLPSVTDHDPAKSAVTTLKDEKEFNTFLRLSSPSVIARLRESF
CEEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHC
PDLPDEPDARTVFAKLRELRNQW
CCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVERIWTGNAYRNFNYLIACPETGEALAIDPLDHEKTLATARVKGWQITQVLNTHEHHD
CCEEEEECCCCEECCEEEEECCCCCCEEEECCCCCCHHHHHHHCCCEEEEHHHHCCCCCC
HTGGNAAVIAATGAKLIAHHKAGGRIAGVDRGVKAGDVIKVGKTVELECLDTPGHTMCHI
CCCCCEEEEEECCCEEEEEECCCCEEECCCCCCCCCCEEEECCEEEEEEECCCCHHHHHH
CLRSHTEQPALFSGDTLFNAGAGNVHNGGDVNALYATFAEQLARLPDDTLVYPGHDYIEN
HHHCCCCCCCEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHCC
NLRFTLAREPDNAAAKALLPSVTDHDPAKSAVTTLKDEKEFNTFLRLSSPSVIARLRESF
CEEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHC
PDLPDEPDARTVFAKLRELRNQW
CCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA