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The map label for this gene is yiaO [H]

Identifier: 91787750

GI number: 91787750

Start: 1932805

End: 1933776

Strand: Reverse

Name: yiaO [H]

Synonym: Bpro_1871

Alternate gene names: 91787750

Gene position: 1933776-1932805 (Counterclockwise)

Preceding gene: 91787751

Following gene: 91787749

Centisome position: 37.19

GC content: 57.82

Gene sequence:

>972_bases
ATGAAACTCAACAAACTCATCATCGGCATGACCATGGCCCTCGGTATTGTCGCCAGCGCCGCTGCACAGACCACGATGAA
AATCAGTATTTCGACGTCGCAGAATTCCCACCAGGGTGTCGCTATTGACACCTTTGCCAAAGAGGTGGAAAAGCGCACCG
GCGGCCGCTACAAGGTTCAGACTTTCTATAGCGGTGCACTGGGCGGCGAACGCGAATCCATCGAAGCAGTCCAACTGGGC
ACCCAGGAACTCGCCTTCTCCTCCACCGGCCCCGTCCCCAACTTTGTACCGGAGACCAAGATTCTCGACGTGCCCTTCCT
GTTTCGCGACAAGGCCCACGCACGCGCCGTACTCGACGGCCCCATCGGCCAGGACCTGCTGGGCAAATTTGACGCCAAGG
GTTTCAAGGCGCTGGCCTGGGGCGAAAACGGCTTTCGCCACATGACCAACAGCAAGCGTGACGTGAAGGGCCCCGAGGAT
CTGAAAGGTCTGAAAATGCGGACCATGGAAAACCCGGTGCATATTGCCGCTTACAAGGGTTTTGGCATCATTACCACGCC
GATGGCATTCCCCGAAGTGTTCACCGCCCTCCAGCAGGGCACGGTCGATGGCCAGGAAAACCCGCTGTCGGTCATCATTG
CGTCCAAGTTTGACCAGGTGCAAAAGCACCTGTCGCTGACGGGCCACGTCTACTCGCCCTGCATCTGGGTGATGAACAAG
GCAGTCTTCGACAAGCTCAGCGCGGCCGACAAGCAGGCGTTTCTTGACGCCGCCAAGGAAGGCACCAAGGCCAACCGCGC
ACGGGTCGATGAGGACGACGCCAAAGGGGTCGCCGACCTGCGCGCCAAGGGCATGACGGTGATTGACAATATTGACAAAT
CGAAATTTGTCACTGCCCTGGCACCGGTCAACGCCGAATTCGAAAAGCAGTTCGGCAAGGCCAACATCGAGAAGATCCGC
AATTACAAGTAA

Upstream 100 bases:

>100_bases
TGTGGCGACTCTGGCCGAACGGACATAATCCAGAGTTGTATGACAACTTTTGACGGGCCGGAAGCCCGTCAGAACGACCA
CCCACCCCTTCGGAGACCCC

Downstream 100 bases:

>100_bases
GCGGTCCAATTGCGCATTGCATTTTCATTTTTTTAGCCCTCTACGCCCGCAGTCACTGGACTGGCGGGCGTTTCATTTGT
GAGCGGAGCGAGTTGCCATG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKLNKLIIGMTMALGIVASAAAQTTMKISISTSQNSHQGVAIDTFAKEVEKRTGGRYKVQTFYSGALGGERESIEAVQLG
TQELAFSSTGPVPNFVPETKILDVPFLFRDKAHARAVLDGPIGQDLLGKFDAKGFKALAWGENGFRHMTNSKRDVKGPED
LKGLKMRTMENPVHIAAYKGFGIITTPMAFPEVFTALQQGTVDGQENPLSVIIASKFDQVQKHLSLTGHVYSPCIWVMNK
AVFDKLSAADKQAFLDAAKEGTKANRARVDEDDAKGVADLRAKGMTVIDNIDKSKFVTALAPVNAEFEKQFGKANIEKIR
NYK

Sequences:

>Translated_323_residues
MKLNKLIIGMTMALGIVASAAAQTTMKISISTSQNSHQGVAIDTFAKEVEKRTGGRYKVQTFYSGALGGERESIEAVQLG
TQELAFSSTGPVPNFVPETKILDVPFLFRDKAHARAVLDGPIGQDLLGKFDAKGFKALAWGENGFRHMTNSKRDVKGPED
LKGLKMRTMENPVHIAAYKGFGIITTPMAFPEVFTALQQGTVDGQENPLSVIIASKFDQVQKHLSLTGHVYSPCIWVMNK
AVFDKLSAADKQAFLDAAKEGTKANRARVDEDDAKGVADLRAKGMTVIDNIDKSKFVTALAPVNAEFEKQFGKANIEKIR
NYK
>Mature_323_residues
MKLNKLIIGMTMALGIVASAAAQTTMKISISTSQNSHQGVAIDTFAKEVEKRTGGRYKVQTFYSGALGGERESIEAVQLG
TQELAFSSTGPVPNFVPETKILDVPFLFRDKAHARAVLDGPIGQDLLGKFDAKGFKALAWGENGFRHMTNSKRDVKGPED
LKGLKMRTMENPVHIAAYKGFGIITTPMAFPEVFTALQQGTVDGQENPLSVIIASKFDQVQKHLSLTGHVYSPCIWVMNK
AVFDKLSAADKQAFLDAAKEGTKANRARVDEDDAKGVADLRAKGMTVIDNIDKSKFVTALAPVNAEFEKQFGKANIEKIR
NYK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=303, Percent_Identity=41.2541254125413, Blast_Score=228, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35200; Mature: 35200

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKLIIGMTMALGIVASAAAQTTMKISISTSQNSHQGVAIDTFAKEVEKRTGGRYKVQ
CCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCEEHHHHHHHHHHHCCCCEEEE
TFYSGALGGERESIEAVQLGTQELAFSSTGPVPNFVPETKILDVPFLFRDKAHARAVLDG
EEECCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCCCCEEECCCHHHCCCCHHHEEECC
PIGQDLLGKFDAKGFKALAWGENGFRHMTNSKRDVKGPEDLKGLKMRTMENPVHIAAYKG
CCCHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCCCHHHCCCHHHHCCCCEEEEEECC
FGIITTPMAFPEVFTALQQGTVDGQENPLSVIIASKFDQVQKHLSLTGHVYSPCIWVMNK
CCEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
AVFDKLSAADKQAFLDAAKEGTKANRARVDEDDAKGVADLRAKGMTVIDNIDKSKFVTAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCEEEECCCCHHHEEEE
APVNAEFEKQFGKANIEKIRNYK
CCCCCHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure
MKLNKLIIGMTMALGIVASAAAQTTMKISISTSQNSHQGVAIDTFAKEVEKRTGGRYKVQ
CCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCEEHHHHHHHHHHHCCCCEEEE
TFYSGALGGERESIEAVQLGTQELAFSSTGPVPNFVPETKILDVPFLFRDKAHARAVLDG
EEECCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCCCCEEECCCHHHCCCCHHHEEECC
PIGQDLLGKFDAKGFKALAWGENGFRHMTNSKRDVKGPEDLKGLKMRTMENPVHIAAYKG
CCCHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCCCHHHCCCHHHHCCCCEEEEEECC
FGIITTPMAFPEVFTALQQGTVDGQENPLSVIIASKFDQVQKHLSLTGHVYSPCIWVMNK
CCEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
AVFDKLSAADKQAFLDAAKEGTKANRARVDEDDAKGVADLRAKGMTVIDNIDKSKFVTAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCEEEECCCCHHHEEEE
APVNAEFEKQFGKANIEKIRNYK
CCCCCHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]