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The map label for this gene is yitF [H]

Identifier: 91787741

GI number: 91787741

Start: 1922352

End: 1923464

Strand: Direct

Name: yitF [H]

Synonym: Bpro_1862

Alternate gene names: 91787741

Gene position: 1922352-1923464 (Clockwise)

Preceding gene: 91787740

Following gene: 91787742

Centisome position: 36.97

GC content: 66.4

Gene sequence:

>1113_bases
ATGAAGATCACTCGAATCGAGCTCCACCCCATCCGTGTGCCGTTCGACATGGGGGCGGCACCGACGGCGTTTGCCGGGAT
GAACTGGACTTCAGTGGACTCCCTGTTCGTGCGCGTTCGCACTGATGCGGGCGTCGATGGCTGGGGCGAGGGATGGGGGC
ACGTTGCCTGTCCGACCACCAGCGCGGCCTTGACGACACTCGTTGGGCCTGCGTTCATCGGGCGCGATGTTTCCGACCGG
TCGCGATTGATGACGGAGATGTTCCACCGTTTCCATATCCATGGTCGAACGGGCCCGGTGGTGTACGCGCTTTCGGCCAT
CGAGATTGCGCTGTGGGACATCGCCGGCAAGTTGGCGTCGTTGCCGATCGCATCGCTGCTGGGGGGCGCGCCGCGTGAGT
TGACCGCTTACGCGAGCCTGCTGCGCTATGCGGAGCCTGAGTTGGTTGCATCGGCCGTCGATCGCGCCTTGGCGCAGGGG
TTTCGCCACATCAAGCTGCACGAAGTCCGCCTGGACACAGTGCGCGCGGCGCGCGCCGCTTGCGGTGAATCGGTATGGCT
CGCGCTCGACACCAACTGTCCGTGGAGCGTGTCCCAGGCTATCGAGCATGCCCGCGCGCTCGAGGCATCGCGTCTGGCCT
GGCTGGAAGAGCCCGTGTGGCCGCCGGAGGATCACGCCGGCCTGGCGCGTGTACGCGCGGCGACGACGATTCCGATCGCC
GCGGGAGAGAACGTTGCCGGTGTGCACGAATTGGCCGCGATGCTGCGGGCTGGGTCGGTCGACATTTGCCAGCCCAGCGT
CATCAAGTTCGGCGGCATTGAGGCGGTGGCACGGGCTGCGATCCTGGCGCGCACGCATGGCGTCGACTACGTGCCGCACT
GTTTCTACTTCGGCCCGGGCTTTCTGGCGTCGCTTCACCTCGCGGCGGCCTTCGCGCCGGAGGTTGCTTTCGAGCTCTTC
TTCGGCGACCTGCAAGCCAGTCCGTACCACGACGCCGTGCGCGCTCGCCAGGGCCGACTGTCAGTGCCGCCTGGGCCGGG
CCTGGGGCTAGACCCGGACATGGAGATTCTGAATCGCTACCGTCTCGGTCCCCCGGTCGTGATCGAAAGCTGA

Upstream 100 bases:

>100_bases
TGGAGCTCTCGATGGCAGCAGGCCCGAACCCCAAAAACGAGTTGCCAAAAGGCCAAGGGCAGGTCACCATATCCGCCGAC
AACATTGAGGAGTTGCGGCC

Downstream 100 bases:

>100_bases
GGGGTCCTGTTTCGCGTCAACATCGAGGAGCGGGGCAAGGTATAAACGCTGAATGACTCTTTCGGTACAGGTTCAAATCC
AAGTCCCCCCTGAAGCACAA

Product: mandelate racemase/muconate lactonizing protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKITRIELHPIRVPFDMGAAPTAFAGMNWTSVDSLFVRVRTDAGVDGWGEGWGHVACPTTSAALTTLVGPAFIGRDVSDR
SRLMTEMFHRFHIHGRTGPVVYALSAIEIALWDIAGKLASLPIASLLGGAPRELTAYASLLRYAEPELVASAVDRALAQG
FRHIKLHEVRLDTVRAARAACGESVWLALDTNCPWSVSQAIEHARALEASRLAWLEEPVWPPEDHAGLARVRAATTIPIA
AGENVAGVHELAAMLRAGSVDICQPSVIKFGGIEAVARAAILARTHGVDYVPHCFYFGPGFLASLHLAAAFAPEVAFELF
FGDLQASPYHDAVRARQGRLSVPPGPGLGLDPDMEILNRYRLGPPVVIES

Sequences:

>Translated_370_residues
MKITRIELHPIRVPFDMGAAPTAFAGMNWTSVDSLFVRVRTDAGVDGWGEGWGHVACPTTSAALTTLVGPAFIGRDVSDR
SRLMTEMFHRFHIHGRTGPVVYALSAIEIALWDIAGKLASLPIASLLGGAPRELTAYASLLRYAEPELVASAVDRALAQG
FRHIKLHEVRLDTVRAARAACGESVWLALDTNCPWSVSQAIEHARALEASRLAWLEEPVWPPEDHAGLARVRAATTIPIA
AGENVAGVHELAAMLRAGSVDICQPSVIKFGGIEAVARAAILARTHGVDYVPHCFYFGPGFLASLHLAAAFAPEVAFELF
FGDLQASPYHDAVRARQGRLSVPPGPGLGLDPDMEILNRYRLGPPVVIES
>Mature_370_residues
MKITRIELHPIRVPFDMGAAPTAFAGMNWTSVDSLFVRVRTDAGVDGWGEGWGHVACPTTSAALTTLVGPAFIGRDVSDR
SRLMTEMFHRFHIHGRTGPVVYALSAIEIALWDIAGKLASLPIASLLGGAPRELTAYASLLRYAEPELVASAVDRALAQG
FRHIKLHEVRLDTVRAARAACGESVWLALDTNCPWSVSQAIEHARALEASRLAWLEEPVWPPEDHAGLARVRAATTIPIA
AGENVAGVHELAAMLRAGSVDICQPSVIKFGGIEAVARAAILARTHGVDYVPHCFYFGPGFLASLHLAAAFAPEVAFELF
FGDLQASPYHDAVRARQGRLSVPPGPGLGLDPDMEILNRYRLGPPVVIES

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=289, Percent_Identity=31.1418685121107, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI226510960, Length=271, Percent_Identity=28.7822878228782, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 39781; Mature: 39781

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRIELHPIRVPFDMGAAPTAFAGMNWTSVDSLFVRVRTDAGVDGWGEGWGHVACPTT
CCEEEEEEEEEECCCCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEECCCC
SAALTTLVGPAFIGRDVSDRSRLMTEMFHRFHIHGRTGPVVYALSAIEIALWDIAGKLAS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCEEEEHHHHHHHHHHHHHHHHH
LPIASLLGGAPRELTAYASLLRYAEPELVASAVDRALAQGFRHIKLHEVRLDTVRAARAA
CCHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHEEEEEHHHHHHHHHHHH
CGESVWLALDTNCPWSVSQAIEHARALEASRLAWLEEPVWPPEDHAGLARVRAATTIPIA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEE
AGENVAGVHELAAMLRAGSVDICQPSVIKFGGIEAVARAAILARTHGVDYVPHCFYFGPG
CCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCH
FLASLHLAAAFAPEVAFELFFGDLQASPYHDAVRARQGRLSVPPGPGLGLDPDMEILNRY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
RLGPPVVIES
CCCCCEEECC
>Mature Secondary Structure
MKITRIELHPIRVPFDMGAAPTAFAGMNWTSVDSLFVRVRTDAGVDGWGEGWGHVACPTT
CCEEEEEEEEEECCCCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEECCCC
SAALTTLVGPAFIGRDVSDRSRLMTEMFHRFHIHGRTGPVVYALSAIEIALWDIAGKLAS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCEEEEHHHHHHHHHHHHHHHHH
LPIASLLGGAPRELTAYASLLRYAEPELVASAVDRALAQGFRHIKLHEVRLDTVRAARAA
CCHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHEEEEEHHHHHHHHHHHH
CGESVWLALDTNCPWSVSQAIEHARALEASRLAWLEEPVWPPEDHAGLARVRAATTIPIA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEE
AGENVAGVHELAAMLRAGSVDICQPSVIKFGGIEAVARAAILARTHGVDYVPHCFYFGPG
CCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCH
FLASLHLAAAFAPEVAFELFFGDLQASPYHDAVRARQGRLSVPPGPGLGLDPDMEILNRY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
RLGPPVVIES
CCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]