| Definition | Polaromonas sp. JS666 chromosome, complete genome. |
|---|---|
| Accession | NC_007948 |
| Length | 5,200,264 |
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The map label for this gene is glnA [H]
Identifier: 91787688
GI number: 91787688
Start: 1870297
End: 1871712
Strand: Direct
Name: glnA [H]
Synonym: Bpro_1807
Alternate gene names: 91787688
Gene position: 1870297-1871712 (Clockwise)
Preceding gene: 91787687
Following gene: 91787689
Centisome position: 35.97
GC content: 59.6
Gene sequence:
>1416_bases ATGGCAAAGACCGTCGCAGACGTCATGAAAATGGTCAAGGAAAACGAAGTCAAGTTTGTTGACTTCCGTTTTACCGACAC CCGGGGTAAAGAGCAGCACGTGACGGTGCCTGTCTCCCACTTTGACGAAGATAAGTTCACCTCGGGCCACGCGTTTGACG GTTCATCGATTGCCGGCTGGAAGGGCATTGAAGCCTCCGACATGCAGCTGATGCCTGATCCCAACACCGCGAACATTGAC CCGTTCTTCGAAGAAACAACGCTGTTCATGAGCTGCGATGTGCTGGAGCCGAGCGACGGCAAGTCTTATGACCGCGACCC CCGCTCCATCGCCAAGCGTGCAGAGTCCTACCTCAAGGCTTCCGGCCTGGGCGATACCGCCTATTTCGGTCCGGAGCCAG AGTTCTTCATCTTTGACGACGTGCGCTGGAACACCGACATGTCGGGCACCTTCTTCAAAATCGAAGAATACGAAGCACCC TGGAACTCTGGCTCCAAGCTCGAAGGCGGCAACCGTGGCCACCGTCCAACCGTCAAGGGCGGCTACTTCCCCGTGCCGCC GGTTGACAGCACGCAGGACATGCGCGCCGAGATGGCCCTGATCCTCGAATCGCTGGGCATTCCGGTCGAAGTGTTCCACC ACGAAGTGGCTGGCGCCGGCCAGAATGAAATCGGCACCCGTTTCAGCACGCTGGTGCAGCGCGCCGACTGGACCCAGATC CTGAAGTATGTGGTGCAGAACGTGGCCAATGCCTACGGCAAAACCGCGACCTTCATGCCCAAGCCCATCGTAGGCGACAA CGGCTCCGGCATGCACGTTCACCAGTCCATCTGGAAAGACGGCAAAAATCTGTTTGCCGGCGACGGCTATGCGGGCCTGT CTGATTTCGCCCTGTACTACATCGGCGGCATCATCAAGCACGCCCGTGCCCTGAATGCGATCACCAACCCCGGCACCAAC AGCTACAAGCGCCTGGTTCCCGGCTATGAGGCACCGGTCAAGCTGGCCTATTCGGCCAAGAACCGCTCGGCTTCGATCCG CATTCCGTATGTGGCGAACCCCAAGGGCCGCCGCATTGAGGCGCGCTTCCCCGATCCACTGGCCAACCCTTACCTGTGCT TCTCCGCACTGATGATGGCCGGCCTCGACGGCGTCGAGAACAAGATCCACCCGGGCGAAGCCGCTACCAAGGACCTCTAC CATCTGCCGCCCGAGGAAGATGCAAAAGTGCCAACCGTGTGCCACAGCCTGGACCAGGCGCTGGACTGCCTCAACGCAGA CCGCGGCTTCCTGACCAAGGGCGGCGTGTTCACCGACAGCATGCTGGACGCCTACATCGAATTGAAGATGGGTGAAGTGA CGCGCCTGCGCATGGCGACGCACCCCATCGAATTCGAGATGTATTACTCCCTGTAA
Upstream 100 bases:
>100_bases GGTTTGGCGAAAACCGATCAGCCATCCGCTGTGGCACAAAAAGTGCATTCACCATCCGAAGACAATTTTTTAACAACCAT CCAAGCAACAGGAGATTTTG
Downstream 100 bases:
>100_bases TCGGGGCTGCAGGCTCGCGCCACTTACCGTTGCTTACGGTTTGTGGCCAAAAAAGGACGGCAATTGCCGTCCTTTTTTAT GGCTGGTGGGTAATAAGGAT
Product: L-glutamine synthetase
Products: NA
Alternate protein names: Glutamate--ammonia ligase [H]
Number of amino acids: Translated: 471; Mature: 470
Protein sequence:
>471_residues MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFTSGHAFDGSSIAGWKGIEASDMQLMPDPNTANID PFFEETTLFMSCDVLEPSDGKSYDRDPRSIAKRAESYLKASGLGDTAYFGPEPEFFIFDDVRWNTDMSGTFFKIEEYEAP WNSGSKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVQRADWTQI LKYVVQNVANAYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTN SYKRLVPGYEAPVKLAYSAKNRSASIRIPYVANPKGRRIEARFPDPLANPYLCFSALMMAGLDGVENKIHPGEAATKDLY HLPPEEDAKVPTVCHSLDQALDCLNADRGFLTKGGVFTDSMLDAYIELKMGEVTRLRMATHPIEFEMYYSL
Sequences:
>Translated_471_residues MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFTSGHAFDGSSIAGWKGIEASDMQLMPDPNTANID PFFEETTLFMSCDVLEPSDGKSYDRDPRSIAKRAESYLKASGLGDTAYFGPEPEFFIFDDVRWNTDMSGTFFKIEEYEAP WNSGSKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVQRADWTQI LKYVVQNVANAYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTN SYKRLVPGYEAPVKLAYSAKNRSASIRIPYVANPKGRRIEARFPDPLANPYLCFSALMMAGLDGVENKIHPGEAATKDLY HLPPEEDAKVPTVCHSLDQALDCLNADRGFLTKGGVFTDSMLDAYIELKMGEVTRLRMATHPIEFEMYYSL >Mature_470_residues AKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFTSGHAFDGSSIAGWKGIEASDMQLMPDPNTANIDP FFEETTLFMSCDVLEPSDGKSYDRDPRSIAKRAESYLKASGLGDTAYFGPEPEFFIFDDVRWNTDMSGTFFKIEEYEAPW NSGSKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVQRADWTQIL KYVVQNVANAYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTNS YKRLVPGYEAPVKLAYSAKNRSASIRIPYVANPKGRRIEARFPDPLANPYLCFSALMMAGLDGVENKIHPGEAATKDLYH LPPEEDAKVPTVCHSLDQALDCLNADRGFLTKGGVFTDSMLDAYIELKMGEVTRLRMATHPIEFEMYYSL
Specific function: Unknown
COG id: COG0174
COG function: function code E; Glutamine synthetase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamine synthetase family [H]
Homologues:
Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=65.5172413793103, Blast_Score=664, Evalue=0.0, Organism=Escherichia coli, GI87081870, Length=389, Percent_Identity=26.7352185089974, Blast_Score=97, Evalue=2e-21,
Paralogues:
None
Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR008147 - InterPro: IPR014746 - InterPro: IPR008146 - InterPro: IPR004809 - InterPro: IPR001637 [H]
Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]
EC number: =6.3.1.2 [H]
Molecular weight: Translated: 52215; Mature: 52083
Theoretical pI: Translated: 5.47; Mature: 5.47
Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.6 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFTSGHAFDGSSIAGW CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCC KGIEASDMQLMPDPNTANIDPFFEETTLFMSCDVLEPSDGKSYDRDPRSIAKRAESYLKA CCCCCCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH SGLGDTAYFGPEPEFFIFDDVRWNTDMSGTFFKIEEYEAPWNSGSKLEGGNRGHRPTVKG CCCCCCCCCCCCCCEEEEECCEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC GYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVQRADWTQI CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH LKYVVQNVANAYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYY HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH IGGIIKHARALNAITNPGTNSYKRLVPGYEAPVKLAYSAKNRSASIRIPYVANPKGRRIE HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEE ARFPDPLANPYLCFSALMMAGLDGVENKIHPGEAATKDLYHLPPEEDAKVPTVCHSLDQA CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH LDCLNADRGFLTKGGVFTDSMLDAYIELKMGEVTRLRMATHPIEFEMYYSL HHHHCCCCCCEECCCCCHHHHHHHHHHEECCCCEEEHHHCCCEEEEEEECC >Mature Secondary Structure AKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFTSGHAFDGSSIAGW CCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCC KGIEASDMQLMPDPNTANIDPFFEETTLFMSCDVLEPSDGKSYDRDPRSIAKRAESYLKA CCCCCCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH SGLGDTAYFGPEPEFFIFDDVRWNTDMSGTFFKIEEYEAPWNSGSKLEGGNRGHRPTVKG CCCCCCCCCCCCCCEEEEECCEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC GYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVQRADWTQI CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH LKYVVQNVANAYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYY HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH IGGIIKHARALNAITNPGTNSYKRLVPGYEAPVKLAYSAKNRSASIRIPYVANPKGRRIE HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEE ARFPDPLANPYLCFSALMMAGLDGVENKIHPGEAATKDLYHLPPEEDAKVPTVCHSLDQA CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH LDCLNADRGFLTKGGVFTDSMLDAYIELKMGEVTRLRMATHPIEFEMYYSL HHHHCCCCCCEECCCCCHHHHHHHHHHEECCCCEEEHHHCCCEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1969923 [H]