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The map label for this gene is tktB [C]

Identifier: 91787355

GI number: 91787355

Start: 1511517

End: 1512356

Strand: Direct

Name: tktB [C]

Synonym: Bpro_1461

Alternate gene names: 91787355

Gene position: 1511517-1512356 (Clockwise)

Preceding gene: 91787354

Following gene: 91787356

Centisome position: 29.07

GC content: 64.64

Gene sequence:

>840_bases
ATGAGCACGGTGGCCTCACTGACGGATTGCGCCTACCGCATTCGCCGCTACGCGCTGCGCATGGGCGAGGTGCAAGGGCA
GGGCTATATTGGCCAGGCGCTGGGGCTGGCCGATGCGCTGGCGGTGGCGTACGGACACGCGCTGAACTATCGGCCGGAAG
ACCCGCAGTGGGAAGGGCGGGATCGGTTTTTGTTGTCGCATGGCCATTACGCGATCGCCCACTACGCGGCCCTCATTGAA
GCGGGTGTGCTGCCCGAGGAAGAGCTCGAAACCTATGGCAGCGACGACAGCCGCCTGCCGATGTCGGGCATGGCCGCTTA
CACACCGGGGATGGAGATGTCAGGGGGCTCGCTGGGCCAGGGCCTGGTCATTGCCGTTGGCATGGCGCTGGGCCTGCGCC
TGAAGCGCAACCCGGCCTTTGTCTACAACTCCATGTCGGATGGTGAACTCGACGAGGGCTCGACCTGGGAGGCCGCCATG
TCAGCCGCGCACCATGGCCTGTCAAATCTCATCTGCATGGTGGACATCAACAACCAGCAGGCCGACGGGTCGTCCGGCCA
GGTGCTGCGTTTTGAGCCGATTGCCGACAAATGGGCGGCCTTTGGCTGGCATGTGCAGCGCGTGAACGGCAACGACCTGG
GCGCCGTGCTGGCCGCCTTTGACGCCGCCCGCCGGTATGACCTGCCCCAGCCGCGCGTGATCCTGCTCGACACCCTGATG
GGCAAGGGCGTGCCCTTTCTGGAGCAGCGCGAAAAAAATCATTTCATCCGGGTCGACCCGCCTGAATGGCAGCAGGCCAT
CGCGATTCTTGACGCCCAACATGCCGGAGCCAGCGAATGA

Upstream 100 bases:

>100_bases
CCAAATCCATCGTGGACGCCTGGCTGCGCAGCGGCTTCGATGGCGGGCGTTCGCAGCCCAAGGTCGACCTGATCCAGGCC
TACGAAGCGGGGCATTCGCT

Downstream 100 bases:

>100_bases
ACACCGTGACAGAAAAGAAACCCCGCCTGACCACGTCCGCCATGATTGCGTCCATCGCGTCCGAGGGACAGCGCACCCGT
GCAGCGCCGTTTGGCAAGGC

Product: transketolase subunit A

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSTVASLTDCAYRIRRYALRMGEVQGQGYIGQALGLADALAVAYGHALNYRPEDPQWEGRDRFLLSHGHYAIAHYAALIE
AGVLPEEELETYGSDDSRLPMSGMAAYTPGMEMSGGSLGQGLVIAVGMALGLRLKRNPAFVYNSMSDGELDEGSTWEAAM
SAAHHGLSNLICMVDINNQQADGSSGQVLRFEPIADKWAAFGWHVQRVNGNDLGAVLAAFDAARRYDLPQPRVILLDTLM
GKGVPFLEQREKNHFIRVDPPEWQQAIAILDAQHAGASE

Sequences:

>Translated_279_residues
MSTVASLTDCAYRIRRYALRMGEVQGQGYIGQALGLADALAVAYGHALNYRPEDPQWEGRDRFLLSHGHYAIAHYAALIE
AGVLPEEELETYGSDDSRLPMSGMAAYTPGMEMSGGSLGQGLVIAVGMALGLRLKRNPAFVYNSMSDGELDEGSTWEAAM
SAAHHGLSNLICMVDINNQQADGSSGQVLRFEPIADKWAAFGWHVQRVNGNDLGAVLAAFDAARRYDLPQPRVILLDTLM
GKGVPFLEQREKNHFIRVDPPEWQQAIAILDAQHAGASE
>Mature_278_residues
STVASLTDCAYRIRRYALRMGEVQGQGYIGQALGLADALAVAYGHALNYRPEDPQWEGRDRFLLSHGHYAIAHYAALIEA
GVLPEEELETYGSDDSRLPMSGMAAYTPGMEMSGGSLGQGLVIAVGMALGLRLKRNPAFVYNSMSDGELDEGSTWEAAMS
AAHHGLSNLICMVDINNQQADGSSGQVLRFEPIADKWAAFGWHVQRVNGNDLGAVLAAFDAARRYDLPQPRVILLDTLMG
KGVPFLEQREKNHFIRVDPPEWQQAIAILDAQHAGASE

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=274, Percent_Identity=34.3065693430657, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI205277463, Length=254, Percent_Identity=35.0393700787402, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI4507521, Length=254, Percent_Identity=35.0393700787402, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI225637459, Length=253, Percent_Identity=26.4822134387352, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI225637461, Length=253, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI225637463, Length=216, Percent_Identity=27.3148148148148, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1788808, Length=263, Percent_Identity=35.361216730038, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994911, Length=262, Percent_Identity=32.824427480916, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=32.1428571428571, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6325331, Length=246, Percent_Identity=34.1463414634146, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6319593, Length=246, Percent_Identity=30.0813008130081, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45551847, Length=258, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550715, Length=258, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24666278, Length=259, Percent_Identity=30.1158301158301, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24645119, Length=220, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30375; Mature: 30243

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVASLTDCAYRIRRYALRMGEVQGQGYIGQALGLADALAVAYGHALNYRPEDPQWEGR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DRFLLSHGHYAIAHYAALIEAGVLPEEELETYGSDDSRLPMSGMAAYTPGMEMSGGSLGQ
CEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
GLVIAVGMALGLRLKRNPAFVYNSMSDGELDEGSTWEAAMSAAHHGLSNLICMVDINNQQ
HHHHHHHHHHCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHCEEEEEECCCCC
ADGSSGQVLRFEPIADKWAAFGWHVQRVNGNDLGAVLAAFDAARRYDLPQPRVILLDTLM
CCCCCCCEEEEECCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHH
GKGVPFLEQREKNHFIRVDPPEWQQAIAILDAQHAGASE
CCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STVASLTDCAYRIRRYALRMGEVQGQGYIGQALGLADALAVAYGHALNYRPEDPQWEGR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DRFLLSHGHYAIAHYAALIEAGVLPEEELETYGSDDSRLPMSGMAAYTPGMEMSGGSLGQ
CEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
GLVIAVGMALGLRLKRNPAFVYNSMSDGELDEGSTWEAAMSAAHHGLSNLICMVDINNQQ
HHHHHHHHHHCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHCEEEEEECCCCC
ADGSSGQVLRFEPIADKWAAFGWHVQRVNGNDLGAVLAAFDAARRYDLPQPRVILLDTLM
CCCCCCCEEEEECCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHH
GKGVPFLEQREKNHFIRVDPPEWQQAIAILDAQHAGASE
CCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]