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The map label for this gene is gatA [H]

Identifier: 91787282

GI number: 91787282

Start: 1423839

End: 1425242

Strand: Reverse

Name: gatA [H]

Synonym: Bpro_1387

Alternate gene names: 91787282

Gene position: 1425242-1423839 (Counterclockwise)

Preceding gene: 91787283

Following gene: 91787281

Centisome position: 27.41

GC content: 60.11

Gene sequence:

>1404_bases
ATGACCGATAAATCTCTTACTTACGCCTCCATAGAGGAGACCCTCGAAGCTTTGACCAGCGGAGCGATCAACAGCGAAGC
ATTGGTCGAAAATCACTTGTCGCGCATAGAGACCTTTGATGTCAAGCTGCACGCTTACGTAGACCTCTATGCTGAAGAGG
CCTTGCAGGCCGCCCGCGGACTGGACAAGCTCAGGCAAGCCGGCCTCACGGTCGGCCCGCTTCACGGTGTGACCATCGCC
GTCAAAGACCTTTTTGAAATCAAGGGGCATGCCATCACCGCGGGGTCCAAATCGCTCCCGCCACGAACCTCCTCCATCAC
GGCGACGGTGGTCGACCGTTTGAGAGCGGCCGGCGCGATCGTCCTGGGAAAGACGCACACGGTGGAATTCGCTTTTGGCG
GCTGGGGAACCAACACCTCCATGGGGACGCCCTGGAACCCCTGGGACCTTGCGATCCACCGCGTTCCTGGCGGCTCCAGC
AGCGGCTCCGCAGTTGCGGTCTCAGCGGGCATGGCCTGCGCCGCACTTGGAACGGACACCGGTGGATCCGTTCGCATCCC
GGCCGGGCTCTGCGGGCTTGTGGGGCTGAAGACGACGCATGGCCTGATCAGCAGATATGGCCTGATTGACCTATGCCCCA
CACATGACACGGTCGGCCCCATCACACGAACCGTGAGGGATTGCGCGACGCTGCTTGACGTCCTGGCGGGGCCCGATCCG
CAAGACGTAGCGTCCCGCTTTTCTCCTTCGCGTTCGGTCTCGAAAGGGCTTGAGAAAGATGTCAGGGGAATGAGGGTTTG
GACCCTTCCCAAGGCTGAACGCGTGGAAGTGGACCCGGAAGTCCTGAAGGCCTACGACAGGTCCCTTTCTGTATTCCTTG
AGCTTGGCATGACCCTGGTGGACAAGCCACTTCCACAAAGCCTCACCCAATCCATGCGCATCGCGGGCAGCCTGATGAGC
GCCGAAGGCTATGCGAATCTCGGCACGCTTTTCGAGCGCAGCGAACTGGAGTTCGACCCGAACATCAAGCGGCGGATCAT
GCTGGGCCGCGATATCGGCGCCGCTCAATACATCGACCTTCTCCGGGACCGTGAGTCCGCGAAGGCCGCGATGCTGGAAG
CCATGGACGGCGTGGATGTCTGCGTATTTCCGACCAACGCGATCACCGCAATTCCGGTAAGTGAGGTCGACGAACTCGCC
ACGCCGCTGTCGAGATTTGGCCGTTTTGTGAACCTACTTGATTTGTGTTCGGTTGCAATTCCTGCAGGACTTTCCGCCAA
CGGCCTCCCGGTCTCCATCCAGGTTATTGGGCGCCCGTTTGCAGAGCCAACGATATTGAGGGCGGCGTTCGCCTTCGAGA
AAGCAACGTCGTGGAACCAATCATTTCCCGCCGGTCTTGACTGA

Upstream 100 bases:

>100_bases
TGGTGGGCGCCTTCTACTTTGCAATTGCTTTTCCCCTGTCAATGGTTTCGCGAGCGCTTGAGAAGCACCTCTCGCGTGGC
CGAAGGGTAATGGGTTCGAC

Downstream 100 bases:

>100_bases
AGTCCTGAGTCTGGCCCCGGTGGGCGATTTCCGGCCATCGGCCTGATCGATGTGAAGGTCTTTTTTGCAAGTCATTTGCC
ACGAGGTTTCCCATGTTGCA

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTDKSLTYASIEETLEALTSGAINSEALVENHLSRIETFDVKLHAYVDLYAEEALQAARGLDKLRQAGLTVGPLHGVTIA
VKDLFEIKGHAITAGSKSLPPRTSSITATVVDRLRAAGAIVLGKTHTVEFAFGGWGTNTSMGTPWNPWDLAIHRVPGGSS
SGSAVAVSAGMACAALGTDTGGSVRIPAGLCGLVGLKTTHGLISRYGLIDLCPTHDTVGPITRTVRDCATLLDVLAGPDP
QDVASRFSPSRSVSKGLEKDVRGMRVWTLPKAERVEVDPEVLKAYDRSLSVFLELGMTLVDKPLPQSLTQSMRIAGSLMS
AEGYANLGTLFERSELEFDPNIKRRIMLGRDIGAAQYIDLLRDRESAKAAMLEAMDGVDVCVFPTNAITAIPVSEVDELA
TPLSRFGRFVNLLDLCSVAIPAGLSANGLPVSIQVIGRPFAEPTILRAAFAFEKATSWNQSFPAGLD

Sequences:

>Translated_467_residues
MTDKSLTYASIEETLEALTSGAINSEALVENHLSRIETFDVKLHAYVDLYAEEALQAARGLDKLRQAGLTVGPLHGVTIA
VKDLFEIKGHAITAGSKSLPPRTSSITATVVDRLRAAGAIVLGKTHTVEFAFGGWGTNTSMGTPWNPWDLAIHRVPGGSS
SGSAVAVSAGMACAALGTDTGGSVRIPAGLCGLVGLKTTHGLISRYGLIDLCPTHDTVGPITRTVRDCATLLDVLAGPDP
QDVASRFSPSRSVSKGLEKDVRGMRVWTLPKAERVEVDPEVLKAYDRSLSVFLELGMTLVDKPLPQSLTQSMRIAGSLMS
AEGYANLGTLFERSELEFDPNIKRRIMLGRDIGAAQYIDLLRDRESAKAAMLEAMDGVDVCVFPTNAITAIPVSEVDELA
TPLSRFGRFVNLLDLCSVAIPAGLSANGLPVSIQVIGRPFAEPTILRAAFAFEKATSWNQSFPAGLD
>Mature_466_residues
TDKSLTYASIEETLEALTSGAINSEALVENHLSRIETFDVKLHAYVDLYAEEALQAARGLDKLRQAGLTVGPLHGVTIAV
KDLFEIKGHAITAGSKSLPPRTSSITATVVDRLRAAGAIVLGKTHTVEFAFGGWGTNTSMGTPWNPWDLAIHRVPGGSSS
GSAVAVSAGMACAALGTDTGGSVRIPAGLCGLVGLKTTHGLISRYGLIDLCPTHDTVGPITRTVRDCATLLDVLAGPDPQ
DVASRFSPSRSVSKGLEKDVRGMRVWTLPKAERVEVDPEVLKAYDRSLSVFLELGMTLVDKPLPQSLTQSMRIAGSLMSA
EGYANLGTLFERSELEFDPNIKRRIMLGRDIGAAQYIDLLRDRESAKAAMLEAMDGVDVCVFPTNAITAIPVSEVDELAT
PLSRFGRFVNLLDLCSVAIPAGLSANGLPVSIQVIGRPFAEPTILRAAFAFEKATSWNQSFPAGLD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=30.4609218436874, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI195972892, Length=471, Percent_Identity=25.6900212314225, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=402, Percent_Identity=30.5970149253731, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=254, Percent_Identity=31.1023622047244, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=248, Percent_Identity=31.8548387096774, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=270, Percent_Identity=30, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=226, Percent_Identity=31.4159292035398, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=226, Percent_Identity=31.4159292035398, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=516, Percent_Identity=21.3178294573643, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=29.6728971962617, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323950, Length=420, Percent_Identity=26.4285714285714, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648113, Length=508, Percent_Identity=29.5275590551181, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=27.1287128712871, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356731, Length=473, Percent_Identity=25.7928118393235, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648435, Length=231, Percent_Identity=32.034632034632, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648437, Length=231, Percent_Identity=32.034632034632, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550774, Length=231, Percent_Identity=32.034632034632, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=26.0521042084168, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24652985, Length=477, Percent_Identity=24.7379454926625, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922090, Length=477, Percent_Identity=24.7379454926625, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652981, Length=477, Percent_Identity=24.7379454926625, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652983, Length=477, Percent_Identity=24.7379454926625, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=24.7002398081535, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=24.7002398081535, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49657; Mature: 49526

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKSLTYASIEETLEALTSGAINSEALVENHLSRIETFDVKLHAYVDLYAEEALQAARG
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHH
LDKLRQAGLTVGPLHGVTIAVKDLFEIKGHAITAGSKSLPPRTSSITATVVDRLRAAGAI
HHHHHHCCCEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCEE
VLGKTHTVEFAFGGWGTNTSMGTPWNPWDLAIHRVPGGSSSGSAVAVSAGMACAALGTDT
EECCCEEEEEEECCCCCCCCCCCCCCCHHEEEEECCCCCCCCCEEEEECCCEEEEECCCC
GGSVRIPAGLCGLVGLKTTHGLISRYGLIDLCPTHDTVGPITRTVRDCATLLDVLAGPDP
CCCEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCH
QDVASRFSPSRSVSKGLEKDVRGMRVWTLPKAERVEVDPEVLKAYDRSLSVFLELGMTLV
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKPLPQSLTQSMRIAGSLMSAEGYANLGTLFERSELEFDPNIKRRIMLGRDIGAAQYIDL
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHEEEECCCCCHHHHHHH
LRDRESAKAAMLEAMDGVDVCVFPTNAITAIPVSEVDELATPLSRFGRFVNLLDLCSVAI
HHHHHHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAGLSANGLPVSIQVIGRPFAEPTILRAAFAFEKATSWNQSFPAGLD
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TDKSLTYASIEETLEALTSGAINSEALVENHLSRIETFDVKLHAYVDLYAEEALQAARG
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHH
LDKLRQAGLTVGPLHGVTIAVKDLFEIKGHAITAGSKSLPPRTSSITATVVDRLRAAGAI
HHHHHHCCCEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCEE
VLGKTHTVEFAFGGWGTNTSMGTPWNPWDLAIHRVPGGSSSGSAVAVSAGMACAALGTDT
EECCCEEEEEEECCCCCCCCCCCCCCCHHEEEEECCCCCCCCCEEEEECCCEEEEECCCC
GGSVRIPAGLCGLVGLKTTHGLISRYGLIDLCPTHDTVGPITRTVRDCATLLDVLAGPDP
CCCEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCH
QDVASRFSPSRSVSKGLEKDVRGMRVWTLPKAERVEVDPEVLKAYDRSLSVFLELGMTLV
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKPLPQSLTQSMRIAGSLMSAEGYANLGTLFERSELEFDPNIKRRIMLGRDIGAAQYIDL
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHEEEECCCCCHHHHHHH
LRDRESAKAAMLEAMDGVDVCVFPTNAITAIPVSEVDELATPLSRFGRFVNLLDLCSVAI
HHHHHHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAGLSANGLPVSIQVIGRPFAEPTILRAAFAFEKATSWNQSFPAGLD
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA