BacMap

Definition	Polaromonas sp. JS666 chromosome, complete genome.
Accession	NC_007948
Length	5,200,264

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The map label for this gene is fadB1 [H]

Identifier: 91787020

GI number: 91787020

Start: 1159603

End: 1160391

Strand: Reverse

Name: fadB1 [H]

Synonym: Bpro_1122

Alternate gene names: 91787020

Gene position: 1160391-1159603 (Counterclockwise)

Preceding gene: 91787021

Following gene: 91787019

Centisome position: 22.31

GC content: 61.22

Gene sequence:

>789_bases
ATGAGCTACGAAACGATTGTGACCTCTACCCAGGGCCCCGACACGCGCAAGGTGGGCATCATCACCCTGAACCGGCCCAA
GCAGCTCAACGCGCTCAATGACCAGCTCATGGATGAACTGGGAAGCGCACTCAAGGGCTTTGATGCGGACAACAGCGTGG
GCTGCATCATCCTGACCGGCAGTGACAAGGCCTTTGCAGCCGGCGCCGACATCAGCGTCATGGCGAACTACGGCTTTGCC
GACGTCTACAACAACGATTTCATCACCCGCAACTGGGAGCAGATCCGCGGCGTTCGCAAGCCGGTGATTGCCGCCGTGAG
CGGTTTTGCGCTGGGCGGCGGTTGCGAGCTGGCCATGATGTGCGACTTCATCATTGCCGCCGACAACGCGAAATTCGGCC
AGCCCGAAATCAAGCTGGGCATCATCCCCGGTGCCGGCGGTACGCAGCGCCTGCCGCGGGCGGTCGGCAAGGCCAAGGCG
ATGGACATGACCCTGACAGGACGCATGATGGACGCCGCCGAGGCAGAACGCGCCGGCCTGGTCAGCCGGGTAGTGCCGCT
GGACAAACTGATGGATGAGGCGTTAGGGGCGGCTCTGATGATTTGCAGTTATGGCCCGATTTCCCTCATGGCCGCCAAGG
AGGCGATCAACCGCGCCTTTGAAAGCGGGCTCAGTGACGGCGTGATGTTTGAGCGACGGCTTTTCCACGCGACCTTCGCC
ACCGATGACCAAAAAGAAGGCATGGACGCCTTTTTGAACAAACGCAAGCCCGTGTTCAGCCATCGCTGA

Upstream 100 bases:

>100_bases
GGAGCGCGTGTCCCGCCCGCACCGCTGTCGGGAAGCTCGCATGACGCCGAAAACCGGCTGGAACGTGGCATGATAAATCG
GTAACAAAGAAGGACTTTCC

Downstream 100 bases:

>100_bases
TACCGGCCTTTTCAAGCGGCTATAATATCGGTCGCGCGGTGATATAGCTCAGTTGGTTAGAGCGCAGCATTCATAATGCT
GATGTCGGTGGTTCAAGTCC

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSYETIVTSTQGPDTRKVGIITLNRPKQLNALNDQLMDELGSALKGFDADNSVGCIILTGSDKAFAAGADISVMANYGFA
DVYNNDFITRNWEQIRGVRKPVIAAVSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKA
MDMTLTGRMMDAAEAERAGLVSRVVPLDKLMDEALGAALMICSYGPISLMAAKEAINRAFESGLSDGVMFERRLFHATFA
TDDQKEGMDAFLNKRKPVFSHR

Sequences:

>Translated_262_residues
MSYETIVTSTQGPDTRKVGIITLNRPKQLNALNDQLMDELGSALKGFDADNSVGCIILTGSDKAFAAGADISVMANYGFA
DVYNNDFITRNWEQIRGVRKPVIAAVSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKA
MDMTLTGRMMDAAEAERAGLVSRVVPLDKLMDEALGAALMICSYGPISLMAAKEAINRAFESGLSDGVMFERRLFHATFA
TDDQKEGMDAFLNKRKPVFSHR
>Mature_261_residues
SYETIVTSTQGPDTRKVGIITLNRPKQLNALNDQLMDELGSALKGFDADNSVGCIILTGSDKAFAAGADISVMANYGFAD
VYNNDFITRNWEQIRGVRKPVIAAVSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKAM
DMTLTGRMMDAAEAERAGLVSRVVPLDKLMDEALGAALMICSYGPISLMAAKEAINRAFESGLSDGVMFERRLFHATFAT
DDQKEGMDAFLNKRKPVFSHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=54.4061302681992, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=36.2204724409449, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=31.9672131147541, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=37.0165745856354, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI20127408, Length=222, Percent_Identity=34.2342342342342, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=34.8017621145374, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI150378541, Length=261, Percent_Identity=31.8007662835249, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI37594469, Length=180, Percent_Identity=33.8888888888889, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594471, Length=180, Percent_Identity=33.8888888888889, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260274832, Length=263, Percent_Identity=26.9961977186312, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI45643119, Length=263, Percent_Identity=26.9961977186312, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI260275230, Length=263, Percent_Identity=26.9961977186312, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI25453481, Length=199, Percent_Identity=28.643216080402, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI51538573, Length=199, Percent_Identity=28.643216080402, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4757966, Length=199, Percent_Identity=28.643216080402, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI51538581, Length=199, Percent_Identity=28.643216080402, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=233, Percent_Identity=26.1802575107296, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI157694516, Length=233, Percent_Identity=26.1802575107296, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757968, Length=223, Percent_Identity=27.3542600896861, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI49355787, Length=223, Percent_Identity=27.3542600896861, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221136756, Length=258, Percent_Identity=27.5193798449612, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136753, Length=258, Percent_Identity=27.5193798449612, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=27.6264591439689, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI261878539, Length=86, Percent_Identity=45.3488372093023, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI116235487, Length=204, Percent_Identity=27.9411764705882, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI62530384, Length=186, Percent_Identity=29.5698924731183, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=195, Percent_Identity=26.1538461538462, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=45.1737451737452, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=38, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=37.9446640316205, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=35.3174603174603, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=31.4393939393939, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788682, Length=186, Percent_Identity=34.4086021505376, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=31.4606741573034, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=54.6184738955823, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=49.4117647058824, Blast_Score=253, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=34.2342342342342, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=260, Percent_Identity=31.9230769230769, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=239, Percent_Identity=33.0543933054393, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=28.740157480315, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=36.8715083798883, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=179, Percent_Identity=36.3128491620112, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=179, Percent_Identity=36.3128491620112, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=36.3128491620112, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=27.6923076923077, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=196, Percent_Identity=33.1632653061224, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=196, Percent_Identity=33.1632653061224, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=195, Percent_Identity=29.7435897435897, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=26.1904761904762, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=234, Percent_Identity=24.3589743589744, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=32.7868852459016, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=53.639846743295, Blast_Score=284, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=53.639846743295, Blast_Score=284, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=36.2204724409449, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19920382, Length=275, Percent_Identity=30.1818181818182, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=30.4, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21357171, Length=251, Percent_Identity=27.0916334661355, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=29.6747967479675, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571729, Length=246, Percent_Identity=28.4552845528455, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571730, Length=246, Percent_Identity=28.4552845528455, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921000, Length=179, Percent_Identity=34.6368715083799, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24583077, Length=179, Percent_Identity=34.6368715083799, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583079, Length=179, Percent_Identity=34.6368715083799, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24654903, Length=226, Percent_Identity=26.9911504424779, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921018, Length=189, Percent_Identity=30.6878306878307, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583165, Length=194, Percent_Identity=29.3814432989691, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28127; Mature: 27996

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYETIVTSTQGPDTRKVGIITLNRPKQLNALNDQLMDELGSALKGFDADNSVGCIILTG
CCCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
SDKAFAAGADISVMANYGFADVYNNDFITRNWEQIRGVRKPVIAAVSGFALGGGCELAMM
CCCCEECCCCEEEEECCCCHHHCCCCCEECCHHHHHHHHHHHHHHHHCEEECCCCHHHHH
CDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKAMDMTLTGRMMDAAEAERAGL
HHHHEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHH
VSRVVPLDKLMDEALGAALMICSYGPISLMAAKEAINRAFESGLSDGVMFERRLFHATFA
HHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
TDDQKEGMDAFLNKRKPVFSHR
CCCHHCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SYETIVTSTQGPDTRKVGIITLNRPKQLNALNDQLMDELGSALKGFDADNSVGCIILTG
CCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
SDKAFAAGADISVMANYGFADVYNNDFITRNWEQIRGVRKPVIAAVSGFALGGGCELAMM
CCCCEECCCCEEEEECCCCHHHCCCCCEECCHHHHHHHHHHHHHHHHCEEECCCCHHHHH
CDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKAMDMTLTGRMMDAAEAERAGL
HHHHEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHH
VSRVVPLDKLMDEALGAALMICSYGPISLMAAKEAINRAFESGLSDGVMFERRLFHATFA
HHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
TDDQKEGMDAFLNKRKPVFSHR
CCCHHCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]