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The map label for this gene is mpl [H]

Identifier: 91786994

GI number: 91786994

Start: 1129349

End: 1130776

Strand: Reverse

Name: mpl [H]

Synonym: Bpro_1096

Alternate gene names: 91786994

Gene position: 1130776-1129349 (Counterclockwise)

Preceding gene: 91787002

Following gene: 91786988

Centisome position: 21.74

GC content: 65.41

Gene sequence:

>1428_bases
ATGCATATACATATCCTGGGAATTTGCGGCACCTTCATGGGCGGCCTCGCCGCGCTGGCACGGGAGGCGGGGCACAAGGT
GACGGGCTGCGACACGGGCGTTTATCCGCCCATGAGCGACCAGCTGCGCGGCTTGGGCATTGAACTGATTGAGGGTTACA
GCGCCGACCAGCTGGCCTTCGGGCCCGACGTGTTCGTGATCGGCAATGTGGTGTCTCGCGCCCGCGGGCCCGACGGCAGC
CCCAAATACCCTTTGATGGAAGCCATCCTGAACAGCGGCCGGCCCTACACGAGCGGCCCGCAATGGCTTTCGGGCCATGT
GCTGCACCATCCCACCCACCACGCGACCGGGCCGCGCCATGTGCTGGCTGTGGCAGGTACCCATGGCAAAACCACGACCA
CGGCCATGCTGACCTGGATCCTCGAGCAAGCGGGGCTGCAACCGGGCTTCCTGGTCGGCGGCGTCCCGCTGAATTTTGGT
GTCTCGGCCCGGCTGGGCGAAGGCAACACCTTTGTGATCGAAGCCGACGAGTACGACACGGCGTTTTTTGACAAGCGCAG
CAAGTTCGTGCACTACCGCCCGCGCACCGCCATTCTGAACAACCTGGAGTTTGACCACGCCGATATCTTTGATGACCTGG
CGGACATCGAGCGGCAATTTCAACACCTGGTGCGCACGGTGCCCTCACAGGGCCGCATCGTGGTGAATGCCGGTGAGGAA
AGCCTGCGGCGCGTGCTGGCCCAGGGCTGCTGGAGTGAGCAGGTACTCTTCGGCAACAGCCCGCTGAATCAAAGCGGCTT
CACCGCCCAGGGCGAACCGAACGACTTCAAGGTGCTCAAGGCCGGCCAGGCAGTGGCCCACGTCCAGTGGGGCATCAGCG
GGGTCCACAACCAGCTCAATGCCCTGGCCGCCATCGCCGCCGCAGAGCACGTGGGCGTGCCACCCGAAGTGGCCGCCCGG
GCGCTGGCCGAGTTCCAGAACGTCAAGCGCCGCATGGAAGTGCGCGGCGTGGTTCATGCGCACGGTGGCGACATCACGGT
GTACGACGATTTCGCCCACCACCCCACGGCCATCCACACCACCGTGGACGGCCTGCGCCGACAGCTCAAGGGGGCCGGAA
AAGACGGAGACCGCATCCTGGCCATCTTTGAGCCGCGCAGCAACACCATGAAGCTGGGCAGCATGACGGCGCAACTGCCC
TGGAGCCTGGAGCAGGCCGACCTGGCGTTTTGCCACGCTGGCGGGCTGGACTGGGATGCGCGCGCTGCGCTGATGCCGAT
GGGCGCACGCGCCCAGGTCGCTGACAACATCGACCAGCTTGTGGCGCAGGTCAAAGCCTCAGCCCGGCCGGGCGACCATT
TGCTGTGCATGAGCAATGGCGGGTTTGGCGGGATTCACGCGAGGCTGCTGGAAGCGCTCAAGCCCTGA

Upstream 100 bases:

>100_bases
TACTCCGAATGGGCTGAAGGCATTTTTCTTTTGAAAGCGGGAATCCCGCAAGCCCCGGCGATGACAAAGTCCATGAGAGC
CCTGCCTAAAATACAGCCCC

Downstream 100 bases:

>100_bases
AGTACGGCGCTCACGGGCAGCGCCAGTAACTCTTGTCGGAAATTCCGGATCGCCTTCGGACCGCGAACGGTGGACAGCGA
AACGGGCGATGCCCCAGCGC

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MHIHILGICGTFMGGLAALAREAGHKVTGCDTGVYPPMSDQLRGLGIELIEGYSADQLAFGPDVFVIGNVVSRARGPDGS
PKYPLMEAILNSGRPYTSGPQWLSGHVLHHPTHHATGPRHVLAVAGTHGKTTTTAMLTWILEQAGLQPGFLVGGVPLNFG
VSARLGEGNTFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFDDLADIERQFQHLVRTVPSQGRIVVNAGEE
SLRRVLAQGCWSEQVLFGNSPLNQSGFTAQGEPNDFKVLKAGQAVAHVQWGISGVHNQLNALAAIAAAEHVGVPPEVAAR
ALAEFQNVKRRMEVRGVVHAHGGDITVYDDFAHHPTAIHTTVDGLRRQLKGAGKDGDRILAIFEPRSNTMKLGSMTAQLP
WSLEQADLAFCHAGGLDWDARAALMPMGARAQVADNIDQLVAQVKASARPGDHLLCMSNGGFGGIHARLLEALKP

Sequences:

>Translated_475_residues
MHIHILGICGTFMGGLAALAREAGHKVTGCDTGVYPPMSDQLRGLGIELIEGYSADQLAFGPDVFVIGNVVSRARGPDGS
PKYPLMEAILNSGRPYTSGPQWLSGHVLHHPTHHATGPRHVLAVAGTHGKTTTTAMLTWILEQAGLQPGFLVGGVPLNFG
VSARLGEGNTFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFDDLADIERQFQHLVRTVPSQGRIVVNAGEE
SLRRVLAQGCWSEQVLFGNSPLNQSGFTAQGEPNDFKVLKAGQAVAHVQWGISGVHNQLNALAAIAAAEHVGVPPEVAAR
ALAEFQNVKRRMEVRGVVHAHGGDITVYDDFAHHPTAIHTTVDGLRRQLKGAGKDGDRILAIFEPRSNTMKLGSMTAQLP
WSLEQADLAFCHAGGLDWDARAALMPMGARAQVADNIDQLVAQVKASARPGDHLLCMSNGGFGGIHARLLEALKP
>Mature_475_residues
MHIHILGICGTFMGGLAALAREAGHKVTGCDTGVYPPMSDQLRGLGIELIEGYSADQLAFGPDVFVIGNVVSRARGPDGS
PKYPLMEAILNSGRPYTSGPQWLSGHVLHHPTHHATGPRHVLAVAGTHGKTTTTAMLTWILEQAGLQPGFLVGGVPLNFG
VSARLGEGNTFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFDDLADIERQFQHLVRTVPSQGRIVVNAGEE
SLRRVLAQGCWSEQVLFGNSPLNQSGFTAQGEPNDFKVLKAGQAVAHVQWGISGVHNQLNALAAIAAAEHVGVPPEVAAR
ALAEFQNVKRRMEVRGVVHAHGGDITVYDDFAHHPTAIHTTVDGLRRQLKGAGKDGDRILAIFEPRSNTMKLGSMTAQLP
WSLEQADLAFCHAGGLDWDARAALMPMGARAQVADNIDQLVAQVKASARPGDHLLCMSNGGFGGIHARLLEALKP

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=478, Percent_Identity=54.18410041841, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1786279, Length=280, Percent_Identity=33.2142857142857, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 51072; Mature: 51072

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAALAREAGHKVTGCDTGVYPPMSDQLRGLGIELIEGYSADQLAF
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHCCCHHEECCCCCCCEEC
GPDVFVIGNVVSRARGPDGSPKYPLMEAILNSGRPYTSGPQWLSGHVLHHPTHHATGPRH
CCCEEEECHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCE
VLAVAGTHGKTTTTAMLTWILEQAGLQPGFLVGGVPLNFGVSARLGEGNTFVIEADEYDT
EEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCC
AFFDKRSKFVHYRPRTAILNNLEFDHADIFDDLADIERQFQHLVRTVPSQGRIVVNAGEE
HHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
SLRRVLAQGCWSEQVLFGNSPLNQSGFTAQGEPNDFKVLKAGQAVAHVQWGISGVHNQLN
HHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEHHHCHHHHHHHHH
ALAAIAAAEHVGVPPEVAARALAEFQNVKRRMEVRGVVHAHGGDITVYDDFAHHPTAIHT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCHHH
TVDGLRRQLKGAGKDGDRILAIFEPRSNTMKLGSMTAQLPWSLEQADLAFCHAGGLDWDA
HHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCEEECCCCCCCHHCEEEEECCCCCCCC
RAALMPMGARAQVADNIDQLVAQVKASARPGDHLLCMSNGGFGGIHARLLEALKP
CHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAALAREAGHKVTGCDTGVYPPMSDQLRGLGIELIEGYSADQLAF
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHCCCHHEECCCCCCCEEC
GPDVFVIGNVVSRARGPDGSPKYPLMEAILNSGRPYTSGPQWLSGHVLHHPTHHATGPRH
CCCEEEECHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCE
VLAVAGTHGKTTTTAMLTWILEQAGLQPGFLVGGVPLNFGVSARLGEGNTFVIEADEYDT
EEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCC
AFFDKRSKFVHYRPRTAILNNLEFDHADIFDDLADIERQFQHLVRTVPSQGRIVVNAGEE
HHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
SLRRVLAQGCWSEQVLFGNSPLNQSGFTAQGEPNDFKVLKAGQAVAHVQWGISGVHNQLN
HHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEHHHCHHHHHHHHH
ALAAIAAAEHVGVPPEVAARALAEFQNVKRRMEVRGVVHAHGGDITVYDDFAHHPTAIHT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCHHH
TVDGLRRQLKGAGKDGDRILAIFEPRSNTMKLGSMTAQLPWSLEQADLAFCHAGGLDWDA
HHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCEEECCCCCCCHHCEEEEECCCCCCCC
RAALMPMGARAQVADNIDQLVAQVKASARPGDHLLCMSNGGFGGIHARLLEALKP
CHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]