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The map label for this gene is hisJ [H]

Identifier: 91786949

GI number: 91786949

Start: 1077273

End: 1078133

Strand: Reverse

Name: hisJ [H]

Synonym: Bpro_1050

Alternate gene names: 91786949

Gene position: 1078133-1077273 (Counterclockwise)

Preceding gene: 91786950

Following gene: 91786948

Centisome position: 20.73

GC content: 59.23

Gene sequence:

>861_bases
ATGCCTTCCATCACGACACGATATTTCCTGCAAACTGCCGGCACCCTGCTGGCCAGCCTGGTGCTGGTGGCCTGCGGCAA
GCAGGAGCCACCGGCACCAGCGGCCACCGCGCCAGCACCCGCCGCCTCGGCGCCCGCGCCGGCAGCCCGGGTGTATGTGG
TCGGCACCGACGCCGCCTATGCCCCTTTTGAATCGCAAAACGAAAAAGGCGAGATCGTTGGTTTTGATATCGACGTGGTC
AAGGCCGTGGCACAAAAAGCCGGCATCGAGGTGAAATTCATCAATACGCCCTGGGAGGGCATCTTCAATTCACTGAACCA
GGGCGACCGCGACCTGCTGGTTTCCGCGATCACGATCACCCCCGAGCGCAAGCAGACCATGGATTTTTCTTCGCCCTACT
TTGACGCACAACAACTGATTGCTGTGAAGAAAGACTCCAAAATCGCCGACTTCAGCCACCTGAAAAAACTCAAGGTGGCC
GTGCAGAACGGCACCACCGGCGACGAGGTGGTCACCAAGCTGCAGGGCAAGGCCAGCCCCAACATCAAACGTTTCGAGTC
CACCCTACTGGCCCTGAAAGAGCTGGAAGCCGGTGGCGTGGATGCCGTAGTGGCTGACAACGGCGTGGTGGTGCACTATG
TCAACAACAACGTCGGCGCCAAGTTCAAGACCATCAGCGACGCCAGCTTTGCGGCCGAGCAATATGGCCTGGCGGTGAAA
AAGGGCAACACCGAATTGCTGGAAAAACTCAACAAGGGCCTGGCCGACATCAAGACCGACGGCACCTACAACAAGATTTA
CGCCCAGTATTTTGGCGGGGCACCGGCGGCACCTGCACCTGCACCCGCGGCATCGAAATAA

Upstream 100 bases:

>100_bases
TGTGACGGGGGTGCCGCAAGGGGTAAACCCGGAGCCTGGCCGGTTTATACTTTTTTGATTTGGCCTGCAGGCCCCATCAA
CCCAACCCAGCGAGGTCTTC

Downstream 100 bases:

>100_bases
GCGAACCGCAAGGAAAACGGGCCCCGTTCGCTTCGGCGTACGGGGCCCGTTTTTTTGATAAAAGTGTGAAGCCCGCCGAT
AAGCCGGATTCTGTGCGCCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MPSITTRYFLQTAGTLLASLVLVACGKQEPPAPAATAPAPAASAPAPAARVYVVGTDAAYAPFESQNEKGEIVGFDIDVV
KAVAQKAGIEVKFINTPWEGIFNSLNQGDRDLLVSAITITPERKQTMDFSSPYFDAQQLIAVKKDSKIADFSHLKKLKVA
VQNGTTGDEVVTKLQGKASPNIKRFESTLLALKELEAGGVDAVVADNGVVVHYVNNNVGAKFKTISDASFAAEQYGLAVK
KGNTELLEKLNKGLADIKTDGTYNKIYAQYFGGAPAAPAPAPAASK

Sequences:

>Translated_286_residues
MPSITTRYFLQTAGTLLASLVLVACGKQEPPAPAATAPAPAASAPAPAARVYVVGTDAAYAPFESQNEKGEIVGFDIDVV
KAVAQKAGIEVKFINTPWEGIFNSLNQGDRDLLVSAITITPERKQTMDFSSPYFDAQQLIAVKKDSKIADFSHLKKLKVA
VQNGTTGDEVVTKLQGKASPNIKRFESTLLALKELEAGGVDAVVADNGVVVHYVNNNVGAKFKTISDASFAAEQYGLAVK
KGNTELLEKLNKGLADIKTDGTYNKIYAQYFGGAPAAPAPAPAASK
>Mature_285_residues
PSITTRYFLQTAGTLLASLVLVACGKQEPPAPAATAPAPAASAPAPAARVYVVGTDAAYAPFESQNEKGEIVGFDIDVVK
AVAQKAGIEVKFINTPWEGIFNSLNQGDRDLLVSAITITPERKQTMDFSSPYFDAQQLIAVKKDSKIADFSHLKKLKVAV
QNGTTGDEVVTKLQGKASPNIKRFESTLLALKELEAGGVDAVVADNGVVVHYVNNNVGAKFKTISDASFAAEQYGLAVKK
GNTELLEKLNKGLADIKTDGTYNKIYAQYFGGAPAAPAPAPAASK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=35.2173913043478, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787088, Length=236, Percent_Identity=33.8983050847458, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787031, Length=245, Percent_Identity=34.6938775510204, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788648, Length=224, Percent_Identity=30.8035714285714, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788228, Length=224, Percent_Identity=28.125, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788649, Length=239, Percent_Identity=27.1966527196653, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30161; Mature: 30029

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSITTRYFLQTAGTLLASLVLVACGKQEPPAPAATAPAPAASAPAPAARVYVVGTDAAY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
APFESQNEKGEIVGFDIDVVKAVAQKAGIEVKFINTPWEGIFNSLNQGDRDLLVSAITIT
CCCCCCCCCCCEEEECHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCHHEEEEEEEEC
PERKQTMDFSSPYFDAQQLIAVKKDSKIADFSHLKKLKVAVQNGTTGDEVVTKLQGKASP
CCHHHCCCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
NIKRFESTLLALKELEAGGVDAVVADNGVVVHYVNNNVGAKFKTISDASFAAEQYGLAVK
CHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCEEEECCCHHHHHHHHCEEEE
KGNTELLEKLNKGLADIKTDGTYNKIYAQYFGGAPAAPAPAPAASK
CCCHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSITTRYFLQTAGTLLASLVLVACGKQEPPAPAATAPAPAASAPAPAARVYVVGTDAAY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
APFESQNEKGEIVGFDIDVVKAVAQKAGIEVKFINTPWEGIFNSLNQGDRDLLVSAITIT
CCCCCCCCCCCEEEECHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCHHEEEEEEEEC
PERKQTMDFSSPYFDAQQLIAVKKDSKIADFSHLKKLKVAVQNGTTGDEVVTKLQGKASP
CCHHHCCCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
NIKRFESTLLALKELEAGGVDAVVADNGVVVHYVNNNVGAKFKTISDASFAAEQYGLAVK
CHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCEEEECCCHHHHHHHHCEEEE
KGNTELLEKLNKGLADIKTDGTYNKIYAQYFGGAPAAPAPAPAASK
CCCHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]