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The map label for this gene is glnH [H]

Identifier: 91786939

GI number: 91786939

Start: 1066209

End: 1066958

Strand: Reverse

Name: glnH [H]

Synonym: Bpro_1040

Alternate gene names: 91786939

Gene position: 1066958-1066209 (Counterclockwise)

Preceding gene: 91786940

Following gene: 91786938

Centisome position: 20.52

GC content: 59.47

Gene sequence:

>750_bases
ATGAAATTTCGTCGCAACTTCCTGCTGGCCAGCCTCACAGCCCTGACCCTCGGCACCACCAGCGCGCATGCACAAGACAA
TGCGCTGCGCGTGGGCACCGATGCCACCTTCCCGCCCATGGAATTCGTGGAAAACGGCAAGCGCACGGGCTTTGACGTCG
AGCTGGTTGAAGCCATCGCCAAGGCGATGGGCAAGCAGGTCGAATGGGTAGATATCGATTTCAAGGGGCTGATCCCCGGC
CTCATGTCCAAGCGTTTTGACATGGCCGTTTCGGCCATTTACATCACCGATGAACGCAAGAAGGTCGTCGATTTCACCAA
CTCCTACTATGCCGGCGGCCTGGTGGCCATGACCAAAGAGGGCAATACCGCCATCAAAAAGCTGGCCGACCTCGAGGGCA
AAAAGGTCACTGTGCAGGTGGGCACCAAGTCGGTGGGCTATCTGCAGGAGCACTATCCCAAGGTGCAACGGATCGAGGTA
GAAAAGAACCAGGAAATGTTCAACCTGGTGGACATTGGCCGTGCCGATGCCGCCGTGACGGGCAAGCCCGCCGCCTTTCA
GTATGTGCGCACGCGCCCGGGCCTGCGCGTGCTGGAAGAGCAGCTGACCACCGAGGAATACGGCATGGCGCTGCGCAAGG
ACACGCCCGAGCTCACCAGGGCGGTGAACGGTGCGATCGCCAAACTCAAGGCCGACGGCACCTATGCGGCCATCGTGAAA
AAATGGTTCAGCAACAGCGCTGGCAAGTAA

Upstream 100 bases:

>100_bases
ACCAGGCGCTGATCCGCATGGCGGTGGTTGCCGCCGCGCGCATCTCGCGCCACCTGACCCTTCACTGATTTCCCTTACCC
CCCAACCGGAGAACCACCCC

Downstream 100 bases:

>100_bases
TCCGGCAACCTTGCGACTGAGCGATGGAACTCGATTTTTCTCCCATCTGGTCCGGCTGGCCCCAGCTGTTGCGTGGTGCG
CTGGTGACCGTGGAAATCAC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKFRRNFLLASLTALTLGTTSAHAQDNALRVGTDATFPPMEFVENGKRTGFDVELVEAIAKAMGKQVEWVDIDFKGLIPG
LMSKRFDMAVSAIYITDERKKVVDFTNSYYAGGLVAMTKEGNTAIKKLADLEGKKVTVQVGTKSVGYLQEHYPKVQRIEV
EKNQEMFNLVDIGRADAAVTGKPAAFQYVRTRPGLRVLEEQLTTEEYGMALRKDTPELTRAVNGAIAKLKADGTYAAIVK
KWFSNSAGK

Sequences:

>Translated_249_residues
MKFRRNFLLASLTALTLGTTSAHAQDNALRVGTDATFPPMEFVENGKRTGFDVELVEAIAKAMGKQVEWVDIDFKGLIPG
LMSKRFDMAVSAIYITDERKKVVDFTNSYYAGGLVAMTKEGNTAIKKLADLEGKKVTVQVGTKSVGYLQEHYPKVQRIEV
EKNQEMFNLVDIGRADAAVTGKPAAFQYVRTRPGLRVLEEQLTTEEYGMALRKDTPELTRAVNGAIAKLKADGTYAAIVK
KWFSNSAGK
>Mature_249_residues
MKFRRNFLLASLTALTLGTTSAHAQDNALRVGTDATFPPMEFVENGKRTGFDVELVEAIAKAMGKQVEWVDIDFKGLIPG
LMSKRFDMAVSAIYITDERKKVVDFTNSYYAGGLVAMTKEGNTAIKKLADLEGKKVTVQVGTKSVGYLQEHYPKVQRIEV
EKNQEMFNLVDIGRADAAVTGKPAAFQYVRTRPGLRVLEEQLTTEEYGMALRKDTPELTRAVNGAIAKLKADGTYAAIVK
KWFSNSAGK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=242, Percent_Identity=38.8429752066116, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1787085, Length=242, Percent_Identity=33.0578512396694, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788228, Length=218, Percent_Identity=37.1559633027523, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=29.9595141700405, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788649, Length=246, Percent_Identity=31.7073170731707, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788648, Length=226, Percent_Identity=31.4159292035398, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786876, Length=240, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27466; Mature: 27466

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFRRNFLLASLTALTLGTTSAHAQDNALRVGTDATFPPMEFVENGKRTGFDVELVEAIA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHH
KAMGKQVEWVDIDFKGLIPGLMSKRFDMAVSAIYITDERKKVVDFTNSYYAGGLVAMTKE
HHHCCCEEEEECCHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHCCCCCCCCCEEEEECC
GNTAIKKLADLEGKKVTVQVGTKSVGYLQEHYPKVQRIEVEKNQEMFNLVDIGRADAAVT
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEC
GKPAAFQYVRTRPGLRVLEEQLTTEEYGMALRKDTPELTRAVNGAIAKLKADGTYAAIVK
CCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHEEEECCCHHHHHHH
KWFSNSAGK
HHHCCCCCC
>Mature Secondary Structure
MKFRRNFLLASLTALTLGTTSAHAQDNALRVGTDATFPPMEFVENGKRTGFDVELVEAIA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHH
KAMGKQVEWVDIDFKGLIPGLMSKRFDMAVSAIYITDERKKVVDFTNSYYAGGLVAMTKE
HHHCCCEEEEECCHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHCCCCCCCCCEEEEECC
GNTAIKKLADLEGKKVTVQVGTKSVGYLQEHYPKVQRIEVEKNQEMFNLVDIGRADAAVT
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEC
GKPAAFQYVRTRPGLRVLEEQLTTEEYGMALRKDTPELTRAVNGAIAKLKADGTYAAIVK
CCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHEEEECCCHHHHHHH
KWFSNSAGK
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]