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The map label for this gene is dmlR [H]

Identifier: 91786624

GI number: 91786624

Start: 736778

End: 737713

Strand: Reverse

Name: dmlR [H]

Synonym: Bpro_0722

Alternate gene names: 91786624

Gene position: 737713-736778 (Counterclockwise)

Preceding gene: 91786634

Following gene: 91786623

Centisome position: 14.19

GC content: 65.06

Gene sequence:

>936_bases
ATGACCCCTGAAATTCAACCTGCTGACCTGGGTTTTTTCTCGGTGCTCGCTGCTGCCGGCAGCCTGAGCGCCGCCGCACG
GGAACTCGGCATCACCACGCCCGCCGTCAGCAAGCATCTGGCGCTGATGGAGTCCCGCGTGGGTGTGCCGCTGGTGAACC
GGACGACCCGGCGCATGAGCCTGACGCCCGAAGGCGAGCTCTACCTGGAGCACGCGCGTCGCATTCTGGGCGAGATCAAC
GACATGGCGGAACTGCTCGGCGTGTCCAGGTCAACGCCGAAGGGTCTGCTCAGGGTCAATGCGACACTCGGCTTCGGGCG
CACGCACGTGGCACCGATCATCTCCAGGTTCGTCCGCAAATATCCGCAGGTCGAGGTGCAGCTGCAGCTGTCGGTCAATC
CTCCACCCTTGACGGACGACTCGTTCGACGTCTGCATCCGATTCGGCGCGCCGCCGGACGCGCGCGTGATCGCCAGGCAC
ATCGCGCCAAACCGGCGGCTGCTGTGCGCGTCACCGGCCTATCTGGCCAAACACGGCACACCCAAGGTACCCAATGACCT
GACGCGGCACAACTGCATCGGCATCCGGCAGGGCGAGGAGGCGTACGGCTTGTGGCGCCTGGCGAGTGGCCGTGGCCGCA
ACGTGACCACCGAAGCCATCAAGACCCGCGGCAACCTGACCACCAACGATGGCGAGATCGCCGTCAACTGGGCGCTCGAC
GGCCACGGCATCCTGATGCGCGCCGAATGGGACATCGAGCGCCACCTGAAAAACGGCCACCTCGTGCAGGTGCTGCCTCA
TTACTACACGCCTGATGCGGACATCCACGCGGTCTATCCGCAGCGCCACCAGTTATCTGCGCGCGTCCGGGCCTTCGTTG
ACTTTGCGGCACTGTCATTCTCGAAACGCCCGGCGGCAAGCAAGCCCAAGCCATAG

Upstream 100 bases:

>100_bases
CAGGCGGCGGTTTCTCCTCTCGACCGCAAGAATTGGACGGTCCTCGCGCACACCGATATAGTGAAGCCATTGTTTACCTG
CATTTAACAATTGAGCTTTC

Downstream 100 bases:

>100_bases
GCCAAGCCGTGGCAGTCAATGCGGCCGGCGGTTGATCTTTCGCCATCGGGTCCGCTCCATGCCGGCCGGACAATCGACAT
CGCCTTTCGAACACCCCTCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTPEIQPADLGFFSVLAAAGSLSAAARELGITTPAVSKHLALMESRVGVPLVNRTTRRMSLTPEGELYLEHARRILGEIN
DMAELLGVSRSTPKGLLRVNATLGFGRTHVAPIISRFVRKYPQVEVQLQLSVNPPPLTDDSFDVCIRFGAPPDARVIARH
IAPNRRLLCASPAYLAKHGTPKVPNDLTRHNCIGIRQGEEAYGLWRLASGRGRNVTTEAIKTRGNLTTNDGEIAVNWALD
GHGILMRAEWDIERHLKNGHLVQVLPHYYTPDADIHAVYPQRHQLSARVRAFVDFAALSFSKRPAASKPKP

Sequences:

>Translated_311_residues
MTPEIQPADLGFFSVLAAAGSLSAAARELGITTPAVSKHLALMESRVGVPLVNRTTRRMSLTPEGELYLEHARRILGEIN
DMAELLGVSRSTPKGLLRVNATLGFGRTHVAPIISRFVRKYPQVEVQLQLSVNPPPLTDDSFDVCIRFGAPPDARVIARH
IAPNRRLLCASPAYLAKHGTPKVPNDLTRHNCIGIRQGEEAYGLWRLASGRGRNVTTEAIKTRGNLTTNDGEIAVNWALD
GHGILMRAEWDIERHLKNGHLVQVLPHYYTPDADIHAVYPQRHQLSARVRAFVDFAALSFSKRPAASKPKP
>Mature_310_residues
TPEIQPADLGFFSVLAAAGSLSAAARELGITTPAVSKHLALMESRVGVPLVNRTTRRMSLTPEGELYLEHARRILGEIND
MAELLGVSRSTPKGLLRVNATLGFGRTHVAPIISRFVRKYPQVEVQLQLSVNPPPLTDDSFDVCIRFGAPPDARVIARHI
APNRRLLCASPAYLAKHGTPKVPNDLTRHNCIGIRQGEEAYGLWRLASGRGRNVTTEAIKTRGNLTTNDGEIAVNWALDG
HGILMRAEWDIERHLKNGHLVQVLPHYYTPDADIHAVYPQRHQLSARVRAFVDFAALSFSKRPAASKPKP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=40.4761904761905, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=35.2542372881356, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=32.6388888888889, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=32.1799307958478, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=28.6689419795222, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=29.757785467128, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=28.2758620689655, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790011, Length=302, Percent_Identity=26.158940397351, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=24.5614035087719, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=151, Percent_Identity=31.1258278145695, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789506, Length=145, Percent_Identity=33.7931034482759, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788706, Length=278, Percent_Identity=23.7410071942446, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=199, Percent_Identity=28.643216080402, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34225; Mature: 34094

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEIQPADLGFFSVLAAAGSLSAAARELGITTPAVSKHLALMESRVGVPLVNRTTRRMS
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCEEEE
LTPEGELYLEHARRILGEINDMAELLGVSRSTPKGLLRVNATLGFGRTHVAPIISRFVRK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHH
YPQVEVQLQLSVNPPPLTDDSFDVCIRFGAPPDARVIARHIAPNRRLLCASPAYLAKHGT
CCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEECCHHHHHCCC
PKVPNDLTRHNCIGIRQGEEAYGLWRLASGRGRNVTTEAIKTRGNLTTNDGEIAVNWALD
CCCCCCCCCCCEEEECCCCHHHCEEEECCCCCCCEEHHHHHHCCCEECCCCCEEEEEEEC
GHGILMRAEWDIERHLKNGHLVQVLPHYYTPDADIHAVYPQRHQLSARVRAFVDFAALSF
CCEEEEEEECCHHHHCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHC
SKRPAASKPKP
CCCCCCCCCCC
>Mature Secondary Structure 
TPEIQPADLGFFSVLAAAGSLSAAARELGITTPAVSKHLALMESRVGVPLVNRTTRRMS
CCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCEEEE
LTPEGELYLEHARRILGEINDMAELLGVSRSTPKGLLRVNATLGFGRTHVAPIISRFVRK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHH
YPQVEVQLQLSVNPPPLTDDSFDVCIRFGAPPDARVIARHIAPNRRLLCASPAYLAKHGT
CCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEECCHHHHHCCC
PKVPNDLTRHNCIGIRQGEEAYGLWRLASGRGRNVTTEAIKTRGNLTTNDGEIAVNWALD
CCCCCCCCCCCEEEECCCCHHHCEEEECCCCCCCEEHHHHHHCCCEECCCCCEEEEEEEC
GHGILMRAEWDIERHLKNGHLVQVLPHYYTPDADIHAVYPQRHQLSARVRAFVDFAALSF
CCEEEEEEECCHHHHCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHC
SKRPAASKPKP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]