BacMap

Definition	Polaromonas sp. JS666 chromosome, complete genome.
Accession	NC_007948
Length	5,200,264

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The map label for this gene is crt [H]

Identifier: 91786554

GI number: 91786554

Start: 657874

End: 658659

Strand: Direct

Name: crt [H]

Synonym: Bpro_0649

Alternate gene names: 91786554

Gene position: 657874-658659 (Clockwise)

Preceding gene: 91786553

Following gene: 91786555

Centisome position: 12.65

GC content: 66.54

Gene sequence:

>786_bases
ATGCTGATAGAAGCCGAGCGCCGCGGCCAGCACGTGGTGCTGACGCTGAACCGCCCCGAAAAACTCAACGCCCTGAGCTA
CGAACTCATCGATGAGCTGATGGACCGGCTCGATGCGCTGGAGCGCGACGAGAGCGTGCGCTGCCTGGTGCTGACCGGGC
GGGGCGACAAGGCCTTCAGCGCCGGCGCCGACATCGCGGGCTTTGCGCCGGATGTTGCCGCATCGCCCGAAACAGCAGCG
CGCCGCTTCGTCGCGCGCGGCCAGGCCATGACGCGCCGCGTCGAGAACTACCCCAAGCCCATCATCGCGGCTGTGAACGG
GATTGCCTTTGGCGGCGGCTGCGAAACCATGGAGGCCTGCAGCCTGGCGATTGCGGCGGAACATGCGACTTTTGCCAAGC
CTGAAATCCGGCTGGGCTTTCCGCCGCCGTTCGGCGGTACACAGCGCCTGCCGCGCCATATAGGCCGCAAGCGCGCCAAC
GAAATGATCCTGACGGGGGAGGCCATCAGCGCGGAAGAGGCATTGCTGATCGGGCTCATCAACCACGTGGTGCCTGCCAG
CGACCTGTTGCCTGCCTGCCTGCGCATGGCGGACCTGATCGCCGCCAATTCGGCCGTAGCCGTCACGGCCTCGCTGCGTG
CGGTCACACGCGGGTTGAACGTCTCCATCGACGAAGGCCTGGCCATCGAAGCCGCGCAGTTCATGATCGCCGCGGCCTCG
CCTGATGCGAAGGAGGGCACGGCGGCCTTTGTGGAGAAGCGCCAGCCGGTGTTTTGCCATCGCTGA

Upstream 100 bases:

>100_bases
CGCCGCAAGCCGCGACCTACTTTGTTGGCGCCCCGCGTGGTTGCGGCGTCCTGTCATTTTTATCCCCGGACACCCCTGCC
CGAAACCCTCTGGAGGCTAC

Downstream 100 bases:

>100_bases
GGCCACCGGCCCGTGGGGCCACAGTAGGGCCACAGTGGGGCAATAGTGAGGTTATTGAGCGGCTGGCCGGGCCTCGCGCC
GATAATCGGCGCATGACTTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLIEAERRGQHVVLTLNRPEKLNALSYELIDELMDRLDALERDESVRCLVLTGRGDKAFSAGADIAGFAPDVAASPETAA
RRFVARGQAMTRRVENYPKPIIAAVNGIAFGGGCETMEACSLAIAAEHATFAKPEIRLGFPPPFGGTQRLPRHIGRKRAN
EMILTGEAISAEEALLIGLINHVVPASDLLPACLRMADLIAANSAVAVTASLRAVTRGLNVSIDEGLAIEAAQFMIAAAS
PDAKEGTAAFVEKRQPVFCHR

Sequences:

>Translated_261_residues
MLIEAERRGQHVVLTLNRPEKLNALSYELIDELMDRLDALERDESVRCLVLTGRGDKAFSAGADIAGFAPDVAASPETAA
RRFVARGQAMTRRVENYPKPIIAAVNGIAFGGGCETMEACSLAIAAEHATFAKPEIRLGFPPPFGGTQRLPRHIGRKRAN
EMILTGEAISAEEALLIGLINHVVPASDLLPACLRMADLIAANSAVAVTASLRAVTRGLNVSIDEGLAIEAAQFMIAAAS
PDAKEGTAAFVEKRQPVFCHR
>Mature_261_residues
MLIEAERRGQHVVLTLNRPEKLNALSYELIDELMDRLDALERDESVRCLVLTGRGDKAFSAGADIAGFAPDVAASPETAA
RRFVARGQAMTRRVENYPKPIIAAVNGIAFGGGCETMEACSLAIAAEHATFAKPEIRLGFPPPFGGTQRLPRHIGRKRAN
EMILTGEAISAEEALLIGLINHVVPASDLLPACLRMADLIAANSAVAVTASLRAVTRGLNVSIDEGLAIEAAQFMIAAAS
PDAKEGTAAFVEKRQPVFCHR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=42.0849420849421, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=36.5384615384615, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=30.3643724696356, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=31.640625, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=34.4444444444444, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI68989263, Length=180, Percent_Identity=35, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=30.1587301587302, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI37594469, Length=203, Percent_Identity=31.5270935960591, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI37594471, Length=203, Percent_Identity=31.5270935960591, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI260274832, Length=174, Percent_Identity=32.183908045977, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45643119, Length=174, Percent_Identity=32.183908045977, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI260275230, Length=174, Percent_Identity=32.183908045977, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI4757966, Length=183, Percent_Identity=30.0546448087432, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI51538581, Length=183, Percent_Identity=30.0546448087432, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI25453481, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI51538573, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI4757968, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI49355787, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=176, Percent_Identity=28.4090909090909, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI213417737, Length=176, Percent_Identity=28.4090909090909, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=197, Percent_Identity=28.4263959390863, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI62530384, Length=187, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=246, Percent_Identity=22.7642276422764, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=39.84375, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=35.9848484848485, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=35.0597609561753, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=36.6141732283465, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=29.5275590551181, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=36.1702127659575, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=36.5079365079365, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=39.6, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=38.8461538461538, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17540714, Length=218, Percent_Identity=32.5688073394495, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=27.9527559055118, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=27.1698113207547, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558304, Length=237, Percent_Identity=29.957805907173, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=234, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=234, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=234, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=264, Percent_Identity=26.8939393939394, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=31.413612565445, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=31.413612565445, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=192, Percent_Identity=31.7708333333333, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=186, Percent_Identity=31.7204301075269, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=265, Percent_Identity=29.0566037735849, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=175, Percent_Identity=27.4285714285714, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=30.0546448087432, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=40.3162055335968, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=40.3162055335968, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=34.2205323193916, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19920382, Length=254, Percent_Identity=30.7086614173228, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=27.4193548387097, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24654903, Length=186, Percent_Identity=31.1827956989247, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571729, Length=198, Percent_Identity=30.8080808080808, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571730, Length=198, Percent_Identity=30.8080808080808, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=202, Percent_Identity=31.1881188118812, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=27.6, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=33.8983050847458, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=33.8983050847458, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=33.8983050847458, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583165, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27851; Mature: 27851

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEAERRGQHVVLTLNRPEKLNALSYELIDELMDRLDALERDESVRCLVLTGRGDKAFS
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
AGADIAGFAPDVAASPETAARRFVARGQAMTRRVENYPKPIIAAVNGIAFGGGCETMEAC
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCHHHHH
SLAIAAEHATFAKPEIRLGFPPPFGGTQRLPRHIGRKRANEMILTGEAISAEEALLIGLI
HHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
NHVVPASDLLPACLRMADLIAANSAVAVTASLRAVTRGLNVSIDEGLAIEAAQFMIAAAS
HHHCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCEEECCCCEEEHHHEEEEECC
PDAKEGTAAFVEKRQPVFCHR
CCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLIEAERRGQHVVLTLNRPEKLNALSYELIDELMDRLDALERDESVRCLVLTGRGDKAFS
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
AGADIAGFAPDVAASPETAARRFVARGQAMTRRVENYPKPIIAAVNGIAFGGGCETMEAC
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCHHHHH
SLAIAAEHATFAKPEIRLGFPPPFGGTQRLPRHIGRKRANEMILTGEAISAEEALLIGLI
HHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
NHVVPASDLLPACLRMADLIAANSAVAVTASLRAVTRGLNVSIDEGLAIEAAQFMIAAAS
HHHCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCEEECCCCEEEHHHEEEEECC
PDAKEGTAAFVEKRQPVFCHR
CCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]