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The map label for this gene is purT

Identifier: 91786540

GI number: 91786540

Start: 642029

End: 643237

Strand: Direct

Name: purT

Synonym: Bpro_0635

Alternate gene names: 91786540

Gene position: 642029-643237 (Clockwise)

Preceding gene: 91786534

Following gene: 91786541

Centisome position: 12.35

GC content: 65.76

Gene sequence:

>1209_bases
ATGACCACTTTCGGAACCCCTTTGTCCCCCCATGCCACCAAAGTCATGCTGCTCGGCTCCGGCGAGCTGGGCAAGGAAGT
GCTGATCGCGCTGCAGCGCCTGGGCGTGGAAACGATTGCCACCGACCGCTACGACAATGCGCCCGGCCAGCAGGTCGCCC
ACCATGCGCGCACCATCACCATGAGCGACCCGGCGCAGCTTAAAAGCCTGATTGAAAAGGAAAGGCCGCACCTGGTCGTT
CCCGAGATCGAGGCCATCGCCACGGCCATGCTCGAGGAACTCGAAGCCGCTGGCACGGTGCGCGTGATTCCCACGGCCCG
CGCCGCCCGCCTGACCATGGACCGCGAAGGCATACGCCGGCTGGCCGCCGAGACCCTGGGCCTGCCGACCAGCCCCTATG
TGTTCTGCGATTCGCTGCAGGAGCTGCAGGCCGCCATCGACACCAACATCGGCTACCCCTGCATCGTCAAGCCCGTCATG
AGTTCCTCGGGCAAGGGCCAGAGCAAGATCGCCGGCCCCGCCGACGTGAAAACCGCCTGGGACTACGCGATGGCCGGTGG
CCGCGTGAGCCACGGCCGGGTGATCGTCGAGGGCTTCATCGACTTTGACTACGAGATCACGCAGCTCACCGTGCGCGCCC
TCGGCGCTGACGGCCAGGTGGAAACGCATTTTTGCGAACCGATTGGCCACATCCAGGTCAGTGGCGACTACGTGGAGAGC
TGGCAGCCGCATCCCATGCGCGAGGCCGCCCTGCAGAAAAGCCGCGCCATCGCCAAGGCCGTGACCGACGACCTCGGCGG
CCAGGGGCTCTTTGGCGTCGAGCTGTTCGTCAAGGGTGACGAGGTCTGGTTCAGCGAAGTCAGCCCGCGCCCGCACGACA
CTGGCATGGTGACGATGTGTACGCAATGGCAAAACGAATTTGAACTGCATGCACGCGCCATCCTGGGCCTGCCGGTCAAC
ACCGCGCTCAAGAGCTCCGGGGCCAGCGCGGTGATTTACGGCGGTGTGGACGCCAAAGGCATTGTGTTCGACGGCGTGGC
CGACGCGCTGCGCGTGCCCAACACCGACATTCGCCTGTTCGGCAAGCCCGAGAGTTTCGTCAAGCGCCGCATGGGCGTGG
CACTGGCGTTTGATGCCGATGTCGAGGTGGCGCGTGCCAACGCCAAGCTGGCCGCGTCCCGGGTCAAGCCCCGGGTGGCG
AAGGGCTGA

Upstream 100 bases:

>100_bases
GCTGGTGCAGTAAAGTAAGCCCAGGCGGTGCCCAGGCGCCCAGCCTTGCGGCCGCCGTTCCCCTTTTCTCCCATTTCTCC
CACTTCTACCTTCAGCGCTT

Downstream 100 bases:

>100_bases
TGCATGCTGACAGGGCTGCGGTATGAGGCTGCGGTATGAACCTGTCTGAGCTCGTCGCCTGGATGGACGACTCGTCGCTG
CGCATCGTGCTGGCAGCCGG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTTFGTPLSPHATKVMLLGSGELGKEVLIALQRLGVETIATDRYDNAPGQQVAHHARTITMSDPAQLKSLIEKERPHLVV
PEIEAIATAMLEELEAAGTVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYVFCDSLQELQAAIDTNIGYPCIVKPVM
SSSGKGQSKIAGPADVKTAWDYAMAGGRVSHGRVIVEGFIDFDYEITQLTVRALGADGQVETHFCEPIGHIQVSGDYVES
WQPHPMREAALQKSRAIAKAVTDDLGGQGLFGVELFVKGDEVWFSEVSPRPHDTGMVTMCTQWQNEFELHARAILGLPVN
TALKSSGASAVIYGGVDAKGIVFDGVADALRVPNTDIRLFGKPESFVKRRMGVALAFDADVEVARANAKLAASRVKPRVA
KG

Sequences:

>Translated_402_residues
MTTFGTPLSPHATKVMLLGSGELGKEVLIALQRLGVETIATDRYDNAPGQQVAHHARTITMSDPAQLKSLIEKERPHLVV
PEIEAIATAMLEELEAAGTVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYVFCDSLQELQAAIDTNIGYPCIVKPVM
SSSGKGQSKIAGPADVKTAWDYAMAGGRVSHGRVIVEGFIDFDYEITQLTVRALGADGQVETHFCEPIGHIQVSGDYVES
WQPHPMREAALQKSRAIAKAVTDDLGGQGLFGVELFVKGDEVWFSEVSPRPHDTGMVTMCTQWQNEFELHARAILGLPVN
TALKSSGASAVIYGGVDAKGIVFDGVADALRVPNTDIRLFGKPESFVKRRMGVALAFDADVEVARANAKLAASRVKPRVA
KG
>Mature_401_residues
TTFGTPLSPHATKVMLLGSGELGKEVLIALQRLGVETIATDRYDNAPGQQVAHHARTITMSDPAQLKSLIEKERPHLVVP
EIEAIATAMLEELEAAGTVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYVFCDSLQELQAAIDTNIGYPCIVKPVMS
SSGKGQSKIAGPADVKTAWDYAMAGGRVSHGRVIVEGFIDFDYEITQLTVRALGADGQVETHFCEPIGHIQVSGDYVESW
QPHPMREAALQKSRAIAKAVTDDLGGQGLFGVELFVKGDEVWFSEVSPRPHDTGMVTMCTQWQNEFELHARAILGLPVNT
ALKSSGASAVIYGGVDAKGIVFDGVADALRVPNTDIRLFGKPESFVKRRMGVALAFDADVEVARANAKLAASRVKPRVAK
G

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=398, Percent_Identity=57.5376884422111, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324702, Length=371, Percent_Identity=23.7196765498652, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_POLSJ (Q12FU8)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547492.1
- ProteinModelPortal:   Q12FU8
- SMR:   Q12FU8
- STRING:   Q12FU8
- GeneID:   4011426
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0635
- NMPDR:   fig|296591.1.peg.513
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q12FU8
- ProtClustDB:   PRK09288
- BioCyc:   PSP296591:BPRO_0635-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43237; Mature: 43106

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 115-115 BINDING 157-157 BINDING 205-205 BINDING 274-274 BINDING 286-286 BINDING 293-293 BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFGTPLSPHATKVMLLGSGELGKEVLIALQRLGVETIATDRYDNAPGQQVAHHARTIT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHEECCCCCCCCHHHHHHHHEEEE
MSDPAQLKSLIEKERPHLVVPEIEAIATAMLEELEAAGTVRVIPTARAARLTMDREGIRR
ECCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHEECCHHHHHH
LAAETLGLPTSPYVFCDSLQELQAAIDTNIGYPCIVKPVMSSSGKGQSKIAGPADVKTAW
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHH
DYAMAGGRVSHGRVIVEGFIDFDYEITQLTVRALGADGQVETHFCEPIGHIQVSGDYVES
HHHHCCCCCCCCEEEEEEEECCCEEEHEEEEEHHCCCCCEEHEECCCCCEEEECCCHHHC
WQPHPMREAALQKSRAIAKAVTDDLGGQGLFGVELFVKGDEVWFSEVSPRPHDTGMVTMC
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEE
TQWQNEFELHARAILGLPVNTALKSSGASAVIYGGVDAKGIVFDGVADALRVPNTDIRLF
ECCCHHHHEEEHEEECCCCHHHHHCCCCCEEEECCCCCCCEEECCHHHHHHCCCCCEEEE
GKPESFVKRRMGVALAFDADVEVARANAKLAASRVKPRVAKG
CCCHHHHHHHHCEEEEECCCCEEECCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTFGTPLSPHATKVMLLGSGELGKEVLIALQRLGVETIATDRYDNAPGQQVAHHARTIT
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHEECCCCCCCCHHHHHHHHEEEE
MSDPAQLKSLIEKERPHLVVPEIEAIATAMLEELEAAGTVRVIPTARAARLTMDREGIRR
ECCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHEECCHHHHHH
LAAETLGLPTSPYVFCDSLQELQAAIDTNIGYPCIVKPVMSSSGKGQSKIAGPADVKTAW
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHH
DYAMAGGRVSHGRVIVEGFIDFDYEITQLTVRALGADGQVETHFCEPIGHIQVSGDYVES
HHHHCCCCCCCCEEEEEEEECCCEEEHEEEEEHHCCCCCEEHEECCCCCEEEECCCHHHC
WQPHPMREAALQKSRAIAKAVTDDLGGQGLFGVELFVKGDEVWFSEVSPRPHDTGMVTMC
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEE
TQWQNEFELHARAILGLPVNTALKSSGASAVIYGGVDAKGIVFDGVADALRVPNTDIRLF
ECCCHHHHEEEHEEECCCCHHHHHCCCCCEEEECCCCCCCEEECCHHHHHHCCCCCEEEE
GKPESFVKRRMGVALAFDADVEVARANAKLAASRVKPRVAKG
CCCHHHHHHHHCEEEEECCCCEEECCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA