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The map label for this gene is queA [H]

Identifier: 91786377

GI number: 91786377

Start: 482651

End: 483712

Strand: Reverse

Name: queA [H]

Synonym: Bpro_0467

Alternate gene names: 91786377

Gene position: 483712-482651 (Counterclockwise)

Preceding gene: 91786379

Following gene: 91786376

Centisome position: 9.3

GC content: 65.63

Gene sequence:

>1062_bases
ATGAAGACGCATTTCAGCCTCAGTGATTTTGATTTCGCACTGCCCGAAGAACTCATTGCCCAGCACCCCGCCGCCCAGCG
CAGCGCCTCGCGCCTGCTCGATGGACGGAGCAGCCCCGCCACAGACCGCCGCTTCGCCGACCTGCCCGACCTGCTGACGC
CGGGCGACCTGCTGGTGTTCAATGACACCCAGGTGGTCAAGGCGCGGCTGTTCGGCCAAAAATCCACCGGCGGGCGGCTG
GAACTGCTGATCGAGCGGGTGTTGCCCGCCCTCGCCGACGCCAGTCATGAAGTCGCCGCGCACATGAAGGTCAGCAAAAA
GCCCCAACCCGGCGCCGTGCTGCAGATGGACGGCGGCTTCACCGCCACCCTGCTGGGCCGCTGGCCGCAGGAGGATGGGC
CGCTGTACCGCTTTCGCTTCAGCAGCGACCCCTATGCGCTGATGGCGCAGCATGGCCATGTGCCGCTGCCGCCCTATATC
ACCCACAGCGACTCTGCCGAGGACGAACTGCGCTACCAGACGGTGTTTGCAAAAAACCCCGGCGCCGTGGCCGCACCCAC
CGCCGCCCTGCACTTTGACGAAGCCATGCTCGCGCAGCTGGAGGCGCGCGGCATCGCGCGCGCCAGCGTGACCCTGCATG
TGGGCGCGGGCACCTTCCAGCCCGTCAAGACCGAAACAATAGCCGACCACACCATGCATTTCGAGCGCTTTGAGGTGCCG
GCCGCCACGCAGCAGGCGATTGCCGCCTGCAAGGCGCGCGGCGGCCGTGTGGTCGCCGTGGGCACCACCACCGTGCGGGC
ACTGGAGTCCCACGCCAAATTCGGTCCGGCCTGCGACGACACCAACATCTTCATCACGCCGGGCTTTGAATTTGCCGTGG
TGGACCTGCTGCTGACCAACTTTCACCTGCCCAAAAGCACGCTGATGATGCTGGTCAGCGCCTTTGCGGGCTATGAACAC
ATCATGGCGCTGTACCAGCATGCGATTGCCGGGCGCTACCGCTTTTTCAGCTATGGCGATGCCATGCTGCTGGCGCGCAA
AGCCCCACGCGCAAGCGCCTGA

Upstream 100 bases:

>100_bases
TTGCTCCGCCTTGTGGGCAACAGCCCACTGCGTTGTCACGCCTTGCCTGGCGCCGCGCGGGCAGCACACTCGGGCGCATC
CAACTGCGGTTTCTAGGTTT

Downstream 100 bases:

>100_bases
ACACACCTGCGACAATCACAAGATGCTCGAATTTGAAGTTCTGAAGACCGACGCAAGGACCGGCGAAGGGGCCAATGCCC
ACCCCGGCTCGCATGCCCGG

Product: S-adenosylmethionine

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKTHFSLSDFDFALPEELIAQHPAAQRSASRLLDGRSSPATDRRFADLPDLLTPGDLLVFNDTQVVKARLFGQKSTGGRL
ELLIERVLPALADASHEVAAHMKVSKKPQPGAVLQMDGGFTATLLGRWPQEDGPLYRFRFSSDPYALMAQHGHVPLPPYI
THSDSAEDELRYQTVFAKNPGAVAAPTAALHFDEAMLAQLEARGIARASVTLHVGAGTFQPVKTETIADHTMHFERFEVP
AATQQAIAACKARGGRVVAVGTTTVRALESHAKFGPACDDTNIFITPGFEFAVVDLLLTNFHLPKSTLMMLVSAFAGYEH
IMALYQHAIAGRYRFFSYGDAMLLARKAPRASA

Sequences:

>Translated_353_residues
MKTHFSLSDFDFALPEELIAQHPAAQRSASRLLDGRSSPATDRRFADLPDLLTPGDLLVFNDTQVVKARLFGQKSTGGRL
ELLIERVLPALADASHEVAAHMKVSKKPQPGAVLQMDGGFTATLLGRWPQEDGPLYRFRFSSDPYALMAQHGHVPLPPYI
THSDSAEDELRYQTVFAKNPGAVAAPTAALHFDEAMLAQLEARGIARASVTLHVGAGTFQPVKTETIADHTMHFERFEVP
AATQQAIAACKARGGRVVAVGTTTVRALESHAKFGPACDDTNIFITPGFEFAVVDLLLTNFHLPKSTLMMLVSAFAGYEH
IMALYQHAIAGRYRFFSYGDAMLLARKAPRASA
>Mature_353_residues
MKTHFSLSDFDFALPEELIAQHPAAQRSASRLLDGRSSPATDRRFADLPDLLTPGDLLVFNDTQVVKARLFGQKSTGGRL
ELLIERVLPALADASHEVAAHMKVSKKPQPGAVLQMDGGFTATLLGRWPQEDGPLYRFRFSSDPYALMAQHGHVPLPPYI
THSDSAEDELRYQTVFAKNPGAVAAPTAALHFDEAMLAQLEARGIARASVTLHVGAGTFQPVKTETIADHTMHFERFEVP
AATQQAIAACKARGGRVVAVGTTTVRALESHAKFGPACDDTNIFITPGFEFAVVDLLLTNFHLPKSTLMMLVSAFAGYEH
IMALYQHAIAGRYRFFSYGDAMLLARKAPRASA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=52.5714285714286, Blast_Score=348, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38440; Mature: 38440

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTHFSLSDFDFALPEELIAQHPAAQRSASRLLDGRSSPATDRRFADLPDLLTPGDLLVF
CCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEE
NDTQVVKARLFGQKSTGGRLELLIERVLPALADASHEVAAHMKVSKKPQPGAVLQMDGGF
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCCEEEEECCCE
TATLLGRWPQEDGPLYRFRFSSDPYALMAQHGHVPLPPYITHSDSAEDELRYQTVFAKNP
EEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEEEEEECCC
GAVAAPTAALHFDEAMLAQLEARGIARASVTLHVGAGTFQPVKTETIADHTMHFERFEVP
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
AATQQAIAACKARGGRVVAVGTTTVRALESHAKFGPACDDTNIFITPGFEFAVVDLLLTN
HHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHH
FHLPKSTLMMLVSAFAGYEHIMALYQHAIAGRYRFFSYGDAMLLARKAPRASA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCC
>Mature Secondary Structure
MKTHFSLSDFDFALPEELIAQHPAAQRSASRLLDGRSSPATDRRFADLPDLLTPGDLLVF
CCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEE
NDTQVVKARLFGQKSTGGRLELLIERVLPALADASHEVAAHMKVSKKPQPGAVLQMDGGF
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCCEEEEECCCE
TATLLGRWPQEDGPLYRFRFSSDPYALMAQHGHVPLPPYITHSDSAEDELRYQTVFAKNP
EEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEEEEEECCC
GAVAAPTAALHFDEAMLAQLEARGIARASVTLHVGAGTFQPVKTETIADHTMHFERFEVP
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
AATQQAIAACKARGGRVVAVGTTTVRALESHAKFGPACDDTNIFITPGFEFAVVDLLLTN
HHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHH
FHLPKSTLMMLVSAFAGYEHIMALYQHAIAGRYRFFSYGDAMLLARKAPRASA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA