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The map label for this gene is purU [H]

Identifier: 91786348

GI number: 91786348

Start: 455166

End: 456014

Strand: Reverse

Name: purU [H]

Synonym: Bpro_0438

Alternate gene names: 91786348

Gene position: 456014-455166 (Counterclockwise)

Preceding gene: 91786351

Following gene: 91786347

Centisome position: 8.77

GC content: 61.13

Gene sequence:

>849_bases
ATGAACCACGCATATATCCTGACCCTGTCCTGCCCGGACCGTAGGGGCATTGTTCACGCCGTTTCCGGTTTTTTGCTGCA
GAACGGCGGCAACATTGAAGAAGCCGCCCAGTACAACGACCATGACACGGGCCTGTTTTTCATGCGGGTGCAGTTTGCCT
GCGACCAACTGACGCACGAGGAGCTGTCGCAGCAGCTCAAGGGCTTTGCCGAGCCCTTCCAGATGAACTGGAAACTGCAC
GCGACCACGCAACCGATGCGCACCGTCATCATGGTCAGCAAGGAAGGCCATTGCCTGAACGACCTGCTGTTTCGCTGCAA
GTCGGGCCTGCTGCCGCTGGACGTCCGCGCCATCGTGTCCAACCACCGGGAGTTCTACCAGCTGGCGGCCAGCTACAACA
TTCCTTTTCACCATTTCCCGGTGACCGCCGCGAGCAAAGCCCAGGTCGAGGACAAGCAGCTGGAAATCATCGAGTCGGAG
GGCGCCGAGCTGGTGGTGCTGGCGCGCTACATGCAGATCCTTTCCAACGACCTGTGCCGCAAACTGGCTGGCCGCGCGAT
CAACATCCACCACTCCTTCCTGCCCAGCTTCAAGGGCGCCAAGCCTTACTACCAGGCGCATGACCGCGGCGTGAAACTGA
TAGGCGCGACCGCCCACTATGTGACGGGCGACCTGGACGAAGGCCCGATCATCGAACAGGACGTGGCGCGCGTGGACCAC
AGCAAGACCGTCGAAGACCTGACGGCCATGGGCCGCGACACCGAAAGCCAGGTGCTGGCGCGCGCCGTCAAGTGGCACAG
CGAGCATCGCGTGCTGCTCAACGGCCACAAGACCGTGATTTTCAAGTAA

Upstream 100 bases:

>100_bases
AAGGTGTAATCTTGCATCCGGTACTTTTGCGCCTCCCCGGCACCCGGGATTCCGGCCGGATCAGGCGATCGGTGGCGGAG
CCTGCGACAATTCATCCCCT

Downstream 100 bases:

>100_bases
CCACCATGGCCGCGCCGGCGAGAATTCCACCGATTCAGTGCCTGCTGACGTTTGAAGCGCTGGCACGGCTGCGCAGCGTG
ACCCAGGCGGCCGAGGAACT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNHAYILTLSCPDRRGIVHAVSGFLLQNGGNIEEAAQYNDHDTGLFFMRVQFACDQLTHEELSQQLKGFAEPFQMNWKLH
ATTQPMRTVIMVSKEGHCLNDLLFRCKSGLLPLDVRAIVSNHREFYQLAASYNIPFHHFPVTAASKAQVEDKQLEIIESE
GAELVVLARYMQILSNDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTGDLDEGPIIEQDVARVDH
SKTVEDLTAMGRDTESQVLARAVKWHSEHRVLLNGHKTVIFK

Sequences:

>Translated_282_residues
MNHAYILTLSCPDRRGIVHAVSGFLLQNGGNIEEAAQYNDHDTGLFFMRVQFACDQLTHEELSQQLKGFAEPFQMNWKLH
ATTQPMRTVIMVSKEGHCLNDLLFRCKSGLLPLDVRAIVSNHREFYQLAASYNIPFHHFPVTAASKAQVEDKQLEIIESE
GAELVVLARYMQILSNDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTGDLDEGPIIEQDVARVDH
SKTVEDLTAMGRDTESQVLARAVKWHSEHRVLLNGHKTVIFK
>Mature_282_residues
MNHAYILTLSCPDRRGIVHAVSGFLLQNGGNIEEAAQYNDHDTGLFFMRVQFACDQLTHEELSQQLKGFAEPFQMNWKLH
ATTQPMRTVIMVSKEGHCLNDLLFRCKSGLLPLDVRAIVSNHREFYQLAASYNIPFHHFPVTAASKAQVEDKQLEIIESE
GAELVVLARYMQILSNDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTGDLDEGPIIEQDVARVDH
SKTVEDLTAMGRDTESQVLARAVKWHSEHRVLLNGHKTVIFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=119, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI209869995, Length=119, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI209869993, Length=119, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=38.3512544802867, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=30.6878306878307, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=162, Percent_Identity=36.4197530864198, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31814; Mature: 31814

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHAYILTLSCPDRRGIVHAVSGFLLQNGGNIEEAAQYNDHDTGLFFMRVQFACDQLTHE
CCCEEEEEEECCCCCCHHHHHHHHHEECCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
ELSQQLKGFAEPFQMNWKLHATTQPMRTVIMVSKEGHCLNDLLFRCKSGLLPLDVRAIVS
HHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHH
NHREFYQLAASYNIPFHHFPVTAASKAQVEDKQLEIIESEGAELVVLARYMQILSNDLCR
CHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHEECCCCCCEEHHHHHHHHHHHHHHH
KLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTGDLDEGPIIEQDVARVDH
HHCCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEHEEECCCCCCCCCHHHHHHCCC
SKTVEDLTAMGRDTESQVLARAVKWHSEHRVLLNGHKTVIFK
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC
>Mature Secondary Structure
MNHAYILTLSCPDRRGIVHAVSGFLLQNGGNIEEAAQYNDHDTGLFFMRVQFACDQLTHE
CCCEEEEEEECCCCCCHHHHHHHHHEECCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
ELSQQLKGFAEPFQMNWKLHATTQPMRTVIMVSKEGHCLNDLLFRCKSGLLPLDVRAIVS
HHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHH
NHREFYQLAASYNIPFHHFPVTAASKAQVEDKQLEIIESEGAELVVLARYMQILSNDLCR
CHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHEECCCCCCEEHHHHHHHHHHHHHHH
KLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTGDLDEGPIIEQDVARVDH
HHCCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEHEEECCCCCCCCCHHHHHHCCC
SKTVEDLTAMGRDTESQVLARAVKWHSEHRVLLNGHKTVIFK
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]