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The map label for this gene is glxR [C]

Identifier: 91786304

GI number: 91786304

Start: 406162

End: 407076

Strand: Reverse

Name: glxR [C]

Synonym: Bpro_0394

Alternate gene names: 91786304

Gene position: 407076-406162 (Counterclockwise)

Preceding gene: 91786305

Following gene: 91786300

Centisome position: 7.83

GC content: 65.46

Gene sequence:

>915_bases
GTGAGCAGCATCAACACCAAAACCTACGAACCTTCTTCCCCGCGCAAAGTTGCATTCCTCGGCCTGGGCGTGATGGGCTA
TCCCATGGCCGGTCATCTGGCGCTCGCCGGCCATCAAGTCACCGTGTACAACCGCAGCCCCGCCAGGTCGACGGCCTGGT
GCGCTGAATTTGCGGCCACGGCCCGGCCATCCAGTGCGACCGTGCCCCGCCTGGCAGCGGCCGGGGCCGATGTGGTGTTC
AGCTGCGTCGGCAACGATGATGACCTGCGTTCCGTCACGCTGGGCCCGGACGGTGCCTTTGCGGGCATGAAGCCCGGCGC
GGTGATGGTGGACCACACCACCGCCTCGGCCGATGTTGCCCGCGAGCTCCATGCGGCTGCACAACAGCTCGGGCTGCACT
TCATTGACGCGCCGGTGTCCGGTGGCCAGGCGGGCGCACAGAACGGCATCCTTACGGTGATGTGCGGCGGCGATGCGGCG
GCGTTCGAGAGCATCAAGCCGGCCGGCATGGCCTTCTCCAGGGCTTTCACACTGATGGGCGACAGCGGCGCGGGCCAGCT
CACCAAGATGGTCAACCAGGTCTGCATTGCCGGCCTGGTGCAGGGCCTGAGTGAAGCCATCGCCTTTGGCCAGAAAGCAG
GGCTGGACATGAACCAGGTACTCGACGTGATCGGCAAGGGTGCAGCCCAAAGCTGGCAGCTCGACAACCGCGGCAAGACC
ATGGCGGCCGATCAGTTCGATTTCGGCTTCGCGGTTGACTGGATGCGCAAGGATCTGGGTCTGGTGCTGGATGAAGCCAG
GCGCAATGGCGCGCGCCTGCCCGTGACAGCCCTGGTGGACCAGTTCTACGCCGATGTGCAGGCCATGGGTGGGCGACGCT
GGGATACGTCCAGCCTGATCAGGCGCCTGAAATAG

Upstream 100 bases:

>100_bases
TCGCCATCGTATAACCGTCCGGGCTGAGCCTGTCGAGGGCCCCAACCTCCCAACGCCTGTCGACAAGCTCAGGGCAAAAG
GACAGCAGAGTCAACACGCC

Downstream 100 bases:

>100_bases
GGCGAGCTGTGAGCGATCGCTGCCGCATCAAACGGCAGCTTGCGGCAAGCGCCGGCACCCGGCCAGGTGCCGGCCTGGAT
CGGGCCCTCAATTGCTGGAC

Product: 2-hydroxy-3-oxopropionate reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSSINTKTYEPSSPRKVAFLGLGVMGYPMAGHLALAGHQVTVYNRSPARSTAWCAEFAATARPSSATVPRLAAAGADVVF
SCVGNDDDLRSVTLGPDGAFAGMKPGAVMVDHTTASADVARELHAAAQQLGLHFIDAPVSGGQAGAQNGILTVMCGGDAA
AFESIKPAGMAFSRAFTLMGDSGAGQLTKMVNQVCIAGLVQGLSEAIAFGQKAGLDMNQVLDVIGKGAAQSWQLDNRGKT
MAADQFDFGFAVDWMRKDLGLVLDEARRNGARLPVTALVDQFYADVQAMGGRRWDTSSLIRRLK

Sequences:

>Translated_304_residues
MSSINTKTYEPSSPRKVAFLGLGVMGYPMAGHLALAGHQVTVYNRSPARSTAWCAEFAATARPSSATVPRLAAAGADVVF
SCVGNDDDLRSVTLGPDGAFAGMKPGAVMVDHTTASADVARELHAAAQQLGLHFIDAPVSGGQAGAQNGILTVMCGGDAA
AFESIKPAGMAFSRAFTLMGDSGAGQLTKMVNQVCIAGLVQGLSEAIAFGQKAGLDMNQVLDVIGKGAAQSWQLDNRGKT
MAADQFDFGFAVDWMRKDLGLVLDEARRNGARLPVTALVDQFYADVQAMGGRRWDTSSLIRRLK
>Mature_303_residues
SSINTKTYEPSSPRKVAFLGLGVMGYPMAGHLALAGHQVTVYNRSPARSTAWCAEFAATARPSSATVPRLAAAGADVVFS
CVGNDDDLRSVTLGPDGAFAGMKPGAVMVDHTTASADVARELHAAAQQLGLHFIDAPVSGGQAGAQNGILTVMCGGDAAA
FESIKPAGMAFSRAFTLMGDSGAGQLTKMVNQVCIAGLVQGLSEAIAFGQKAGLDMNQVLDVIGKGAAQSWQLDNRGKTM
AADQFDFGFAVDWMRKDLGLVLDEARRNGARLPVTALVDQFYADVQAMGGRRWDTSSLIRRLK

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=27.7777777777778, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI23308751, Length=300, Percent_Identity=28.3333333333333, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=33.9100346020761, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=35.8885017421603, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=28.9115646258503, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789092, Length=227, Percent_Identity=32.5991189427313, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=27.1062271062271, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574115, Length=292, Percent_Identity=25.3424657534247, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24655230, Length=300, Percent_Identity=29, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922568, Length=300, Percent_Identity=29, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31801; Mature: 31669

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSINTKTYEPSSPRKVAFLGLGVMGYPMAGHLALAGHQVTVYNRSPARSTAWCAEFAAT
CCCCCCCCCCCCCCCEEEEEECCHHCCCCCCCEEEECCEEEEEECCCCCHHHHHHHHHHH
ARPSSATVPRLAAAGADVVFSCVGNDDDLRSVTLGPDGAFAGMKPGAVMVDHTTASADVA
CCCCCCCCCHHHHCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHH
RELHAAAQQLGLHFIDAPVSGGQAGAQNGILTVMCGGDAAAFESIKPAGMAFSRAFTLMG
HHHHHHHHHHCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCHHHHHHEEEEC
DSGAGQLTKMVNQVCIAGLVQGLSEAIAFGQKAGLDMNQVLDVIGKGAAQSWQLDNRGKT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEECCCCCE
MAADQFDFGFAVDWMRKDLGLVLDEARRNGARLPVTALVDQFYADVQAMGGRRWDTSSLI
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
RRLK
HHCC
>Mature Secondary Structure 
SSINTKTYEPSSPRKVAFLGLGVMGYPMAGHLALAGHQVTVYNRSPARSTAWCAEFAAT
CCCCCCCCCCCCCCEEEEEECCHHCCCCCCCEEEECCEEEEEECCCCCHHHHHHHHHHH
ARPSSATVPRLAAAGADVVFSCVGNDDDLRSVTLGPDGAFAGMKPGAVMVDHTTASADVA
CCCCCCCCCHHHHCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHH
RELHAAAQQLGLHFIDAPVSGGQAGAQNGILTVMCGGDAAAFESIKPAGMAFSRAFTLMG
HHHHHHHHHHCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCHHHHHHEEEEC
DSGAGQLTKMVNQVCIAGLVQGLSEAIAFGQKAGLDMNQVLDVIGKGAAQSWQLDNRGKT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEECCCCCE
MAADQFDFGFAVDWMRKDLGLVLDEARRNGARLPVTALVDQFYADVQAMGGRRWDTSSLI
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
RRLK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA