BacMap

Definition	Polaromonas sp. JS666 chromosome, complete genome.
Accession	NC_007948
Length	5,200,264

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The map label for this gene is gra-orf6 [H]

Identifier: 91786268

GI number: 91786268

Start: 374331

End: 375095

Strand: Direct

Name: gra-orf6 [H]

Synonym: Bpro_0358

Alternate gene names: 91786268

Gene position: 374331-375095 (Clockwise)

Preceding gene: 91786267

Following gene: 91786269

Centisome position: 7.2

GC content: 57.25

Gene sequence:

>765_bases
TTGACTATTTCAACACTCCCTTTATCTGGCCAAGTTGCTGTCGTCGTGGGTGGCGGCGGAGGTATTGGTAAGGCTGCAGC
GGGCCGCCTTGCTCGTCTGGGCGCCAGAGTTGCCCTTCTAGATCGGGCAGATGATGCCGAACTCAAAAGGCTGGCTGACG
ACCTTCCCGGTGCGGGACACGGTGGCGTGTTCGCTCAAATCGATGATAGCGAGACCTTGGTAGCAGCGGCGTCCGCGGTT
CAGGCCAGATGGGGAAGGGCAGACATCCTGGTAAATGCCGCAGGCTTTACCAAGCCAATTCCTCATAAAGATCTGGATGC
ATTGACCGATGAGCTGATCGATCAGATGTTTGCCGTGAACTGGAGGGGGCAATTTGCAGCCATTCGCGCATTCGCACCCC
TCCTTCGTCAGAACGGGCAAGGTCTGGTTGTGAATATCTCGTCAATCGCTGCGACGACCGGGGTCGGCAGTAATGTCGCG
TACTGCGCGCTCAAAGCAGGGATCGACGTCATGGCCTCCTGTTTGGGGCGAGCGCTGGCTCCGGATATTCGCGTCATGAA
CGTATCTCCAGGCGTGGTCGACACGACGTTTGTACCAGGGCGCGATCAAGCCTGGAATGACAAGCAGGCCGTTACGACCC
CGCTTAGGCGCATAGGTTCTACGGAGGACATTGCTGCGGCTATCGAAGCATGCGCCACAACTTTGAAATTCTCCACCGGA
ACTGTTTTGCAGGTGGATGGTGGTCGTCACCTCGGAACACCCTAA

Upstream 100 bases:

>100_bases
TCCAAGAAAAAGATGGTTGTTATTCGCTGAACTATGACTTCGTATTGCAAGAGGGAATTTCAAAGTTCCCAGAACCCCCC
ATTAAATAGAGAGCCAAATT

Downstream 100 bases:

>100_bases
TAGACAAATCCATGGCTCATAAAACCATCATTACCTGTGCAGTCACAGGAAACATCACGACGCCGGATCAACATCCCGGC
TTGCCCATCACGCCAGAACA

Product: short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORF6 [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTISTLPLSGQVAVVVGGGGGIGKAAAGRLARLGARVALLDRADDAELKRLADDLPGAGHGGVFAQIDDSETLVAAASAV
QARWGRADILVNAAGFTKPIPHKDLDALTDELIDQMFAVNWRGQFAAIRAFAPLLRQNGQGLVVNISSIAATTGVGSNVA
YCALKAGIDVMASCLGRALAPDIRVMNVSPGVVDTTFVPGRDQAWNDKQAVTTPLRRIGSTEDIAAAIEACATTLKFSTG
TVLQVDGGRHLGTP

Sequences:

>Translated_254_residues
MTISTLPLSGQVAVVVGGGGGIGKAAAGRLARLGARVALLDRADDAELKRLADDLPGAGHGGVFAQIDDSETLVAAASAV
QARWGRADILVNAAGFTKPIPHKDLDALTDELIDQMFAVNWRGQFAAIRAFAPLLRQNGQGLVVNISSIAATTGVGSNVA
YCALKAGIDVMASCLGRALAPDIRVMNVSPGVVDTTFVPGRDQAWNDKQAVTTPLRRIGSTEDIAAAIEACATTLKFSTG
TVLQVDGGRHLGTP
>Mature_253_residues
TISTLPLSGQVAVVVGGGGGIGKAAAGRLARLGARVALLDRADDAELKRLADDLPGAGHGGVFAQIDDSETLVAAASAVQ
ARWGRADILVNAAGFTKPIPHKDLDALTDELIDQMFAVNWRGQFAAIRAFAPLLRQNGQGLVVNISSIAATTGVGSNVAY
CALKAGIDVMASCLGRALAPDIRVMNVSPGVVDTTFVPGRDQAWNDKQAVTTPLRRIGSTEDIAAAIEACATTLKFSTGT
VLQVDGGRHLGTP

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=256, Percent_Identity=33.984375, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI15277342, Length=253, Percent_Identity=32.0158102766798, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=32.4675324675325, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI32483357, Length=253, Percent_Identity=31.2252964426877, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=32.1285140562249, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI7705925, Length=251, Percent_Identity=30.2788844621514, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI142976729, Length=198, Percent_Identity=28.7878787878788, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI40254992, Length=247, Percent_Identity=29.9595141700405, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI83715985, Length=262, Percent_Identity=31.6793893129771, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40807363, Length=175, Percent_Identity=32, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=28.030303030303, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=34.3589743589744, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=28.6821705426357, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=30.4, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=30.6201550387597, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787905, Length=260, Percent_Identity=30.3846153846154, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=31.2, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=30.1960784313725, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786812, Length=261, Percent_Identity=30.2681992337165, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082160, Length=266, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=26.4, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=263, Percent_Identity=32.6996197718631, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=31.2741312741313, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17555706, Length=244, Percent_Identity=31.5573770491803, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538182, Length=253, Percent_Identity=32.8063241106719, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193203117, Length=215, Percent_Identity=31.1627906976744, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=29.7959183673469, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI193203115, Length=211, Percent_Identity=30.8056872037915, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=264, Percent_Identity=28.7878787878788, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=249, Percent_Identity=30.1204819277108, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=249, Percent_Identity=26.9076305220884, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=161, Percent_Identity=29.8136645962733, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=238, Percent_Identity=27.7310924369748, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=27.843137254902, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=239, Percent_Identity=28.8702928870293, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=270, Percent_Identity=26.2962962962963, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=163, Percent_Identity=26.3803680981595, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=30.4182509505703, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=30.2238805970149, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=32.03125, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI23397609, Length=258, Percent_Identity=32.5581395348837, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=32.156862745098, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24644339, Length=267, Percent_Identity=29.9625468164794, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24639444, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17737361, Length=263, Percent_Identity=29.6577946768061, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355319, Length=231, Percent_Identity=29.4372294372294, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=29.8804780876494, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640492, Length=196, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644337, Length=263, Percent_Identity=31.5589353612167, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364892, Length=166, Percent_Identity=28.9156626506024, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581726, Length=161, Percent_Identity=32.2981366459627, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 26121; Mature: 25990

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISTLPLSGQVAVVVGGGGGIGKAAAGRLARLGARVALLDRADDAELKRLADDLPGAGH
CEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCC
GGVFAQIDDSETLVAAASAVQARWGRADILVNAAGFTKPIPHKDLDALTDELIDQMFAVN
CCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
WRGQFAAIRAFAPLLRQNGQGLVVNISSIAATTGVGSNVAYCALKAGIDVMASCLGRALA
CCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCEEHHHHHCHHHHHHHHHHHHC
PDIRVMNVSPGVVDTTFVPGRDQAWNDKQAVTTPLRRIGSTEDIAAAIEACATTLKFSTG
CCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCC
TVLQVDGGRHLGTP
CEEEECCCCCCCCC
>Mature Secondary Structure 
TISTLPLSGQVAVVVGGGGGIGKAAAGRLARLGARVALLDRADDAELKRLADDLPGAGH
EEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCC
GGVFAQIDDSETLVAAASAVQARWGRADILVNAAGFTKPIPHKDLDALTDELIDQMFAVN
CCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
WRGQFAAIRAFAPLLRQNGQGLVVNISSIAATTGVGSNVAYCALKAGIDVMASCLGRALA
CCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCEEHHHHHCHHHHHHHHHHHHC
PDIRVMNVSPGVVDTTFVPGRDQAWNDKQAVTTPLRRIGSTEDIAAAIEACATTLKFSTG
CCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCC
TVLQVDGGRHLGTP
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 9831526 [H]