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The map label for this gene is purR [H]

Identifier: 91786254

GI number: 91786254

Start: 363740

End: 364756

Strand: Reverse

Name: purR [H]

Synonym: Bpro_0343

Alternate gene names: 91786254

Gene position: 364756-363740 (Counterclockwise)

Preceding gene: 91786258

Following gene: 91786250

Centisome position: 7.01

GC content: 55.06

Gene sequence:

>1017_bases
ATGAATCTGAAAGAACTTGCTAAGTCCCTGGGAGTCCACGCGTCCACTGTGTCCCGAGTATTGAATCCGGCAACTCGGCA
CATGGTTGGAAAGGAGGTCGCTGCCAAAGTGTTAGCTGCAGCGGCCCGCCACAATTTCACGCCAAACAATGTTGCTTCGT
CTCTTCGTACGCGCCGTTCCAATATTGTTGGCATCGTTCTGCCGGATATTTCGAACCCCGTTTTCCCCCCCATCCTTCTG
GGCATCGAGACCGAGCTCCGAAAGCACGGCCTCGTGGCACTGCTGGCGAACGGCGGGGAAGATCCTAAGCAACAACGCAC
GGTGGTTGATCAACTACTGGCGCGCCAAGTCGACGCCTTGATTTTGGCGACCGCAACGCGCAAAGATCGCCTGATCGACC
ATTGCCTTTCCAAGAATACTGTCGTTGTCACCATCAACCGAGGCGAGGATCATGGGCGGGTATCGAGCGTCATGAACGAC
GAGCTGTTAGGCATGCGCTTGGCGTTTGATCATCTCGTGTCCCTTGGGCATACAGAAATCGTGCATATCGTCGGCCCGCA
AAATCTCTCTACAGGCTATTTGCGTCGTGCCGGGTTCATTCAAGCTGCAGTCGCCAACGGCTGGCAAAAGAGCAAGCTCA
ACATGGTCGATTGCACCGCCTACACACGTGAGGCCGGCAGAGTTGCGTGCCAAACCCTCCTCAAGACTTTTCCAAAAACC
ACTGCCGTGCTAGCCGGCAATGACCTTGTGGCTCTAGGCTGCTACGACGTCCTCCTGGAAAAGAAGATCAAATGCCCACG
GGACATGTCAATCGTGGGATACAACGACATGCCCTTCGTGGACTTGGTCTCGCCGGCGCTGACGACAGTTCGTATTCGCC
ACCACGAAATGGGCATTCAAGCAGCTCAGCTGATTCTCCGGCGAATCCAGCAAGGTCAAGATGGTGAAGCGGAAATTAGG
CTGAAGCCCGAGCTGGTAGTTCGGCAGTCCACAAGGTCTCCACGAAAACAAGATTAG

Upstream 100 bases:

>100_bases
TCAGCCTCCCGTCCGGCCTTCGCTCAGGCTCATTACCTGATTTGGAAAATACTTCTCGACAGCTGGTAAGCTTGCATTCG
TTTGCATAAATGGTTATCCG

Downstream 100 bases:

>100_bases
TCGGCCCTGCAAAATCGGCTTGCGGATCACGCACCGTCCATGACGCGTCCCGAAGCCGCACCTCACGCTGTCGTTGCGCC
GAGGACAGAAGTGTTCAGAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNLKELAKSLGVHASTVSRVLNPATRHMVGKEVAAKVLAAAARHNFTPNNVASSLRTRRSNIVGIVLPDISNPVFPPILL
GIETELRKHGLVALLANGGEDPKQQRTVVDQLLARQVDALILATATRKDRLIDHCLSKNTVVVTINRGEDHGRVSSVMND
ELLGMRLAFDHLVSLGHTEIVHIVGPQNLSTGYLRRAGFIQAAVANGWQKSKLNMVDCTAYTREAGRVACQTLLKTFPKT
TAVLAGNDLVALGCYDVLLEKKIKCPRDMSIVGYNDMPFVDLVSPALTTVRIRHHEMGIQAAQLILRRIQQGQDGEAEIR
LKPELVVRQSTRSPRKQD

Sequences:

>Translated_338_residues
MNLKELAKSLGVHASTVSRVLNPATRHMVGKEVAAKVLAAAARHNFTPNNVASSLRTRRSNIVGIVLPDISNPVFPPILL
GIETELRKHGLVALLANGGEDPKQQRTVVDQLLARQVDALILATATRKDRLIDHCLSKNTVVVTINRGEDHGRVSSVMND
ELLGMRLAFDHLVSLGHTEIVHIVGPQNLSTGYLRRAGFIQAAVANGWQKSKLNMVDCTAYTREAGRVACQTLLKTFPKT
TAVLAGNDLVALGCYDVLLEKKIKCPRDMSIVGYNDMPFVDLVSPALTTVRIRHHEMGIQAAQLILRRIQQGQDGEAEIR
LKPELVVRQSTRSPRKQD
>Mature_338_residues
MNLKELAKSLGVHASTVSRVLNPATRHMVGKEVAAKVLAAAARHNFTPNNVASSLRTRRSNIVGIVLPDISNPVFPPILL
GIETELRKHGLVALLANGGEDPKQQRTVVDQLLARQVDALILATATRKDRLIDHCLSKNTVVVTINRGEDHGRVSSVMND
ELLGMRLAFDHLVSLGHTEIVHIVGPQNLSTGYLRRAGFIQAAVANGWQKSKLNMVDCTAYTREAGRVACQTLLKTFPKT
TAVLAGNDLVALGCYDVLLEKKIKCPRDMSIVGYNDMPFVDLVSPALTTVRIRHHEMGIQAAQLILRRIQQGQDGEAEIR
LKPELVVRQSTRSPRKQD

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=27.7611940298507, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=28.3185840707965, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786268, Length=310, Percent_Identity=26.1290322580645, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=26.969696969697, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=27.3273273273273, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=28.0120481927711, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=27.5659824046921, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789068, Length=299, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=25.2427184466019, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=25.8675078864353, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787906, Length=319, Percent_Identity=25.0783699059561, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=24.1269841269841, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=24.5283018867925, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37078; Mature: 37078

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKELAKSLGVHASTVSRVLNPATRHMVGKEVAAKVLAAAARHNFTPNNVASSLRTRRS
CCHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
NIVGIVLPDISNPVFPPILLGIETELRKHGLVALLANGGEDPKQQRTVVDQLLARQVDAL
CEEEEEECCCCCCCCCHHEECCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ILATATRKDRLIDHCLSKNTVVVTINRGEDHGRVSSVMNDELLGMRLAFDHLVSLGHTEI
HHHHCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VHIVGPQNLSTGYLRRAGFIQAAVANGWQKSKLNMVDCTAYTREAGRVACQTLLKTFPKT
EEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
TAVLAGNDLVALGCYDVLLEKKIKCPRDMSIVGYNDMPFVDLVSPALTTVRIRHHEMGIQ
EEEEECCCEEEHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHEEEHHHHHHHH
AAQLILRRIQQGQDGEAEIRLKPELVVRQSTRSPRKQD
HHHHHHHHHHCCCCCCEEEEECHHHEEECCCCCCCCCC
>Mature Secondary Structure
MNLKELAKSLGVHASTVSRVLNPATRHMVGKEVAAKVLAAAARHNFTPNNVASSLRTRRS
CCHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
NIVGIVLPDISNPVFPPILLGIETELRKHGLVALLANGGEDPKQQRTVVDQLLARQVDAL
CEEEEEECCCCCCCCCHHEECCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ILATATRKDRLIDHCLSKNTVVVTINRGEDHGRVSSVMNDELLGMRLAFDHLVSLGHTEI
HHHHCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VHIVGPQNLSTGYLRRAGFIQAAVANGWQKSKLNMVDCTAYTREAGRVACQTLLKTFPKT
EEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
TAVLAGNDLVALGCYDVLLEKKIKCPRDMSIVGYNDMPFVDLVSPALTTVRIRHHEMGIQ
EEEEECCCEEEHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHEEEHHHHHHHH
AAQLILRRIQQGQDGEAEIRLKPELVVRQSTRSPRKQD
HHHHHHHHHHCCCCCCEEEEECHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA