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The map label for this gene is lipA [H]

Identifier: 91786224

GI number: 91786224

Start: 337859

End: 338932

Strand: Reverse

Name: lipA [H]

Synonym: Bpro_0313

Alternate gene names: 91786224

Gene position: 338932-337859 (Counterclockwise)

Preceding gene: 91786225

Following gene: 91786223

Centisome position: 6.52

GC content: 62.29

Gene sequence:

>1074_bases
ATGAGCGGATTACCCAGCCCTTCGACAGGCTCAGGGCAAACGGCACCCGACATGAGCACATCAGAAGCCACCAATCCAGT
TGTCCGCGACGTCCAGGCCCTGGAAACCTACAACCCGCTGGCCAAGCAGAAGGCGGCGGCCAAGCTGTCGCGCATTCCGG
TCAAGGTGGAACGCGCCGAGGTGCTGAAGAAGCCCGACTGGATTCGCGTCAAGGCCGGCAGCCCAACCACCCGCTTTTAC
GAGATCAAGCAGATCCTGCGGGAAAACAAGCTCAACACCGTGTGCGAAGAAGCCAGTTGCCCCAACATCGGCGAGTGTTT
CGGCAAGGGCACCGCCACCTTCATGATCATGGGCGACAAATGCACGCGCCGCTGCCCGTTTTGCGACGTCGGCCACGGCC
GGCCCGATCCGCTGGACGTGAATGAGCCCGAGAACCTGGCCAAAACCATTGCCGCACTCAGGCTCAAATACGTCGTGATC
ACCAGCGTGGACCGCGACGACCTGCGCGACGGCGGCAGCGGGCATTTTGTCGAATGCATCCAGAAAATTCGTGAACTGTC
GCCCGGCACCACCATCGAAATCCTGGTGCCCGACTTCCGCGGCCGCGACGACCGCGCGCTGGAAATCCTCAAGGCTGCAC
CGCCTGACGTGATGAACCACAACCTCGAGACCGCGCCGCGCCTGTACAAGGAAGCGCGTCCCGGCAGCGACTATCAGTTC
TCCCTGAACCTGCTCAAGAAATTCAAGGCGCTACACCCCAACGTGCCCACCAAGAGCGGCATCATGGTCGGCCTGGGTGA
AACCGACGAAGAGATTTTGCAGGTCATGCGTGACATGCGCACCCACAACATCGACATGCTGACCATTGGCCAGTACCTGG
CACCGTCGACCAGCCACCTGCCGGTGCGCCGCTATGTGCACCCCGACACCTTCAGGATGTTTGAAGAGGAAGCCTACAAG
ATGGGCTTTACCCACGCCGCCGTGGGTGCAATGGTGCGCTCCAGCTACCACGCCGACCAGCAGGCCCACGCCGCGGGTGT
CGCCGACAAGGCCGACGCCGCATCGGCGCCCTGA

Upstream 100 bases:

>100_bases
AAGAGGCTGCGGACGTGTTGGGGCAGAAGCTGTCAAGTTATCTGGCACCCTGATTTCCCGCTCGGGCTGAGCCTGTCCAA
GCCCCCGGTTCACACCGGCC

Downstream 100 bases:

>100_bases
CCCATGGACAGGCCGCAGGGCTCGCTGTCGCTACGCCGCTACGGCGCCTCGCACGGCAGCCATGCGCATGCGCACTTTCA
GGTGCTGCTCGGACTGGAGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSGLPSPSTGSGQTAPDMSTSEATNPVVRDVQALETYNPLAKQKAAAKLSRIPVKVERAEVLKKPDWIRVKAGSPTTRFY
EIKQILRENKLNTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAALRLKYVVI
TSVDRDDLRDGGSGHFVECIQKIRELSPGTTIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQF
SLNLLKKFKALHPNVPTKSGIMVGLGETDEEILQVMRDMRTHNIDMLTIGQYLAPSTSHLPVRRYVHPDTFRMFEEEAYK
MGFTHAAVGAMVRSSYHADQQAHAAGVADKADAASAP

Sequences:

>Translated_357_residues
MSGLPSPSTGSGQTAPDMSTSEATNPVVRDVQALETYNPLAKQKAAAKLSRIPVKVERAEVLKKPDWIRVKAGSPTTRFY
EIKQILRENKLNTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAALRLKYVVI
TSVDRDDLRDGGSGHFVECIQKIRELSPGTTIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQF
SLNLLKKFKALHPNVPTKSGIMVGLGETDEEILQVMRDMRTHNIDMLTIGQYLAPSTSHLPVRRYVHPDTFRMFEEEAYK
MGFTHAAVGAMVRSSYHADQQAHAAGVADKADAASAP
>Mature_356_residues
SGLPSPSTGSGQTAPDMSTSEATNPVVRDVQALETYNPLAKQKAAAKLSRIPVKVERAEVLKKPDWIRVKAGSPTTRFYE
IKQILRENKLNTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAALRLKYVVIT
SVDRDDLRDGGSGHFVECIQKIRELSPGTTIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFS
LNLLKKFKALHPNVPTKSGIMVGLGETDEEILQVMRDMRTHNIDMLTIGQYLAPSTSHLPVRRYVHPDTFRMFEEEAYKM
GFTHAAVGAMVRSSYHADQQAHAAGVADKADAASAP

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=44.4067796610169, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=45.5252918287938, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1786846, Length=309, Percent_Identity=62.4595469255663, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32564533, Length=255, Percent_Identity=45.0980392156863, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=45.0171821305842, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=47.0383275261324, Blast_Score=254, Evalue=8e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 39377; Mature: 39246

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLPSPSTGSGQTAPDMSTSEATNPVVRDVQALETYNPLAKQKAAAKLSRIPVKVERAE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEHHHH
VLKKPDWIRVKAGSPTTRFYEIKQILRENKLNTVCEEASCPNIGECFGKGTATFMIMGDK
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCH
CTRRCPFCDVGHGRPDPLDVNEPENLAKTIAALRLKYVVITSVDRDDLRDGGSGHFVECI
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHCCCCCCHHHHHH
QKIRELSPGTTIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQF
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEE
SLNLLKKFKALHPNVPTKSGIMVGLGETDEEILQVMRDMRTHNIDMLTIGQYLAPSTSHL
HHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCC
PVRRYVHPDTFRMFEEEAYKMGFTHAAVGAMVRSSYHADQQAHAAGVADKADAASAP
CHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SGLPSPSTGSGQTAPDMSTSEATNPVVRDVQALETYNPLAKQKAAAKLSRIPVKVERAE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEHHHH
VLKKPDWIRVKAGSPTTRFYEIKQILRENKLNTVCEEASCPNIGECFGKGTATFMIMGDK
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCH
CTRRCPFCDVGHGRPDPLDVNEPENLAKTIAALRLKYVVITSVDRDDLRDGGSGHFVECI
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHCCCCCCHHHHHH
QKIRELSPGTTIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQF
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEE
SLNLLKKFKALHPNVPTKSGIMVGLGETDEEILQVMRDMRTHNIDMLTIGQYLAPSTSHL
HHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCC
PVRRYVHPDTFRMFEEEAYKMGFTHAAVGAMVRSSYHADQQAHAAGVADKADAASAP
CHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA