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The map label for this gene is phbI [H]

Identifier: 91786210

GI number: 91786210

Start: 313062

End: 314864

Strand: Direct

Name: phbI [H]

Synonym: Bpro_0299

Alternate gene names: 91786210

Gene position: 313062-314864 (Clockwise)

Preceding gene: 91786209

Following gene: 91786211

Centisome position: 6.02

GC content: 64.73

Gene sequence:

>1803_bases
ATGACGTTCTCAGTCCACGGTCTGGCGGTCTCGCGGGGTATTGCGATTGGCCGTGCCGTGCTCGTGGCGTCCAGCCGTGC
CGATGTGGCGCACTACTTCGTCGACCCCGCCAGGGTCGTTGAAGAGATCACGCGCGCCCGGGTGGCCCGCAATGCCGTGG
CGGAAGAAATTACCCGCCTGCAGCAGGAGCTGCCGCCGGATGCGCCGCACGAACTGGCTGCCCTGCTGGACGTTCACCTG
ATGCTGCTGCAGGACGAGCAGCTGATCAGCGGCGTGAAGCACTGGATCACCGAACGCCATTACAACGCCGAGTGGGCATT
GGCCACGCAGTACGAAATCATTGCCCGCCAGTTCGATGACATGGAAGACGAGTACCTGCGTGAGCGCAAGGCCGACCTGG
AGCAGGTGGTGGAACGCATCCTGCGGTACATGAAAGGCGTGGCCTCACCGGTGCAGCCGGTGGGGCCTGCCGGCGCCGGC
CGCAAGCTCTCGCAGGGGCTGCTGCTGGACGACACCATGGATGTGCCCCTGGTGCTGATCGCGCACGACATCTCCCCGGC
CGACATGCTGCAGTTCAAGAAGAGCCTGTTTGCCGGTTTTGCGACCGACGTTGGCGGCAAGACCTCGCACACGGCGATCG
TGGCGCGCAGCATGGACATCCCGGCGGTGGTGGGCGCGCGCAGCGCCAGCCAGCTGATCGAGCAGGACGACTGGGTCATC
ATCGACGGCGACGCCGGTGTGCTGATTGTCGACCCTTCACCCATCATCCTGGCCGAGTACGGTTTCAAGCAGCGCCAGGG
TGAGCTGGAGCGCGAGCGGCTGAACCGGCTCAAGCACACCCCCGCCGTGACGATAGACGGACAGAGGGTGGAGCTGCTGG
CCAATATTGAAATGCCCGAGGACACCGTGGGCGCGGTCAATGCGGGTGCCGTCGGCGTCGGGCTGTTCCGCAGCGAGTTC
CTGTTCATGGGGCGCAGTGGCGACCTCCCCGATGAGGAGGAGCAGTACCAGGCTTACCGCAAAGCTGTTGAGGGCATGCA
CGGCCTGCCGGTGACCATTCGCACCGTTGATGTGGGCTCGGACAAACCGCTGGACCGGATCGACAAGGCGCAGGACAGCC
ACCTCAATCCGGCGCTGGGCCTGCGCGCCATCCGCTGGAGCCTGGCCGACCCGCCGATGTTTTTGACGCAGCTGCGTGCC
ATCCTGCGCGCGGCGGCGCATGGCCAGGTCAATTTGCTGGTGCCCATGCTGGCCCACGGCACCGAGATTCGCCAGACCAT
GGCGATGATTGATCACGCACGCGCCACGCTGGACAACAAGGGCACGCCCTATGGCCCGGTGCGTCTGGGCGCCATGATCG
AAATTCCGGCGGCTGCGCTCTCGCTCAAGCTTTTCCTCAAATACTTCGACTTTCTGTCCATAGGCACCAATGACCTCATC
CAGTACACACTGGCGATTGACCGGGCGGACGAGTCCGTGGCGCATTTGTACGACCCTTGCCATCCGGCGGTGCTGCGTCT
GGTGGCCGACACGATCGCCGAATGCAATGCGCAGGGCAAAGGGGTCAGCGTGTGCGGGGAAATGGCTGGCGATGTGAGCA
TGACGCGGCTGCTGCTGGGGCTGGGATTGCGCAGCTTTTCCATGCATCCGTCGCGCATCCTGGCGGTCAAGCAGCAAATT
TTGCGGGCTGATGCCGGCAAGCTCAGCGTCTGGGCCGCGCAGGTGCTGGATGCCGAGGATCCGGCCGCCCTGATAAACCC
TGCCTCAGCGCCCATGCCATCGCCTGTGCCAGCCAACGCCTAG

Upstream 100 bases:

>100_bases
TGGGGCTGCCCCGCGGAAAATGATGTTGCCCTTCTTCGTGCCTGCCACACGTATTGACGTATTGAGCATGGCATCTGTTA
ATTGGAATAGGGGAATTTGA

Downstream 100 bases:

>100_bases
CCGGTCGGGCCGGGGGATCGCCGGTTGAAAATCGACCGCAGCGGTAATATTCCCGTTTTCCCCGTTTTTTTGCCCCATGG
CGAGGCTACGAAACCGGGTC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTFSVHGLAVSRGIAIGRAVLVASSRADVAHYFVDPARVVEEITRARVARNAVAEEITRLQQELPPDAPHELAALLDVHL
MLLQDEQLISGVKHWITERHYNAEWALATQYEIIARQFDDMEDEYLRERKADLEQVVERILRYMKGVASPVQPVGPAGAG
RKLSQGLLLDDTMDVPLVLIAHDISPADMLQFKKSLFAGFATDVGGKTSHTAIVARSMDIPAVVGARSASQLIEQDDWVI
IDGDAGVLIVDPSPIILAEYGFKQRQGELERERLNRLKHTPAVTIDGQRVELLANIEMPEDTVGAVNAGAVGVGLFRSEF
LFMGRSGDLPDEEEQYQAYRKAVEGMHGLPVTIRTVDVGSDKPLDRIDKAQDSHLNPALGLRAIRWSLADPPMFLTQLRA
ILRAAAHGQVNLLVPMLAHGTEIRQTMAMIDHARATLDNKGTPYGPVRLGAMIEIPAAALSLKLFLKYFDFLSIGTNDLI
QYTLAIDRADESVAHLYDPCHPAVLRLVADTIAECNAQGKGVSVCGEMAGDVSMTRLLLGLGLRSFSMHPSRILAVKQQI
LRADAGKLSVWAAQVLDAEDPAALINPASAPMPSPVPANA

Sequences:

>Translated_600_residues
MTFSVHGLAVSRGIAIGRAVLVASSRADVAHYFVDPARVVEEITRARVARNAVAEEITRLQQELPPDAPHELAALLDVHL
MLLQDEQLISGVKHWITERHYNAEWALATQYEIIARQFDDMEDEYLRERKADLEQVVERILRYMKGVASPVQPVGPAGAG
RKLSQGLLLDDTMDVPLVLIAHDISPADMLQFKKSLFAGFATDVGGKTSHTAIVARSMDIPAVVGARSASQLIEQDDWVI
IDGDAGVLIVDPSPIILAEYGFKQRQGELERERLNRLKHTPAVTIDGQRVELLANIEMPEDTVGAVNAGAVGVGLFRSEF
LFMGRSGDLPDEEEQYQAYRKAVEGMHGLPVTIRTVDVGSDKPLDRIDKAQDSHLNPALGLRAIRWSLADPPMFLTQLRA
ILRAAAHGQVNLLVPMLAHGTEIRQTMAMIDHARATLDNKGTPYGPVRLGAMIEIPAAALSLKLFLKYFDFLSIGTNDLI
QYTLAIDRADESVAHLYDPCHPAVLRLVADTIAECNAQGKGVSVCGEMAGDVSMTRLLLGLGLRSFSMHPSRILAVKQQI
LRADAGKLSVWAAQVLDAEDPAALINPASAPMPSPVPANA
>Mature_599_residues
TFSVHGLAVSRGIAIGRAVLVASSRADVAHYFVDPARVVEEITRARVARNAVAEEITRLQQELPPDAPHELAALLDVHLM
LLQDEQLISGVKHWITERHYNAEWALATQYEIIARQFDDMEDEYLRERKADLEQVVERILRYMKGVASPVQPVGPAGAGR
KLSQGLLLDDTMDVPLVLIAHDISPADMLQFKKSLFAGFATDVGGKTSHTAIVARSMDIPAVVGARSASQLIEQDDWVII
DGDAGVLIVDPSPIILAEYGFKQRQGELERERLNRLKHTPAVTIDGQRVELLANIEMPEDTVGAVNAGAVGVGLFRSEFL
FMGRSGDLPDEEEQYQAYRKAVEGMHGLPVTIRTVDVGSDKPLDRIDKAQDSHLNPALGLRAIRWSLADPPMFLTQLRAI
LRAAAHGQVNLLVPMLAHGTEIRQTMAMIDHARATLDNKGTPYGPVRLGAMIEIPAAALSLKLFLKYFDFLSIGTNDLIQ
YTLAIDRADESVAHLYDPCHPAVLRLVADTIAECNAQGKGVSVCGEMAGDVSMTRLLLGLGLRSFSMHPSRILAVKQQIL
RADAGKLSVWAAQVLDAEDPAALINPASAPMPSPVPANA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=35.4275741710297, Blast_Score=336, Evalue=3e-93,
Organism=Escherichia coli, GI1789193, Length=552, Percent_Identity=33.5144927536232, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1788726, Length=592, Percent_Identity=30.7432432432432, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI48994992, Length=504, Percent_Identity=33.1349206349206, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1787994, Length=458, Percent_Identity=24.4541484716157, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI226510935, Length=182, Percent_Identity=24.1758241758242, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65353; Mature: 65222

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSVHGLAVSRGIAIGRAVLVASSRADVAHYFVDPARVVEEITRARVARNAVAEEITRL
CEEEEEHHHHHCCHHHHHHHEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQELPPDAPHELAALLDVHLMLLQDEQLISGVKHWITERHYNAEWALATQYEIIARQFDD
HHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCC
MEDEYLRERKADLEQVVERILRYMKGVASPVQPVGPAGAGRKLSQGLLLDDTMDVPLVLI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEECCCCCCCEEEE
AHDISPADMLQFKKSLFAGFATDVGGKTSHTAIVARSMDIPAVVGARSASQLIEQDDWVI
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHCCCCEEE
IDGDAGVLIVDPSPIILAEYGFKQRQGELERERLNRLKHTPAVTIDGQRVELLANIEMPE
EECCCCEEEECCCCEEEECCCCHHHCCHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCC
DTVGAVNAGAVGVGLFRSEFLFMGRSGDLPDEEEQYQAYRKAVEGMHGLPVTIRTVDVGS
HHCCCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCC
DKPLDRIDKAQDSHLNPALGLRAIRWSLADPPMFLTQLRAILRAAAHGQVNLLVPMLAHG
CCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
TEIRQTMAMIDHARATLDNKGTPYGPVRLGAMIEIPAAALSLKLFLKYFDFLSIGTNDLI
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECEEEECCHHHHHHHHHHHHHHHHHCCCHHHE
QYTLAIDRADESVAHLYDPCHPAVLRLVADTIAECNAQGKGVSVCGEMAGDVSMTRLLLG
EEHHEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHH
LGLRSFSMHPSRILAVKQQILRADAGKLSVWAAQVLDAEDPAALINPASAPMPSPVPANA
HCCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFSVHGLAVSRGIAIGRAVLVASSRADVAHYFVDPARVVEEITRARVARNAVAEEITRL
EEEEEHHHHHCCHHHHHHHEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQELPPDAPHELAALLDVHLMLLQDEQLISGVKHWITERHYNAEWALATQYEIIARQFDD
HHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCC
MEDEYLRERKADLEQVVERILRYMKGVASPVQPVGPAGAGRKLSQGLLLDDTMDVPLVLI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEECCCCCCCEEEE
AHDISPADMLQFKKSLFAGFATDVGGKTSHTAIVARSMDIPAVVGARSASQLIEQDDWVI
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHCCCCEEE
IDGDAGVLIVDPSPIILAEYGFKQRQGELERERLNRLKHTPAVTIDGQRVELLANIEMPE
EECCCCEEEECCCCEEEECCCCHHHCCHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCC
DTVGAVNAGAVGVGLFRSEFLFMGRSGDLPDEEEQYQAYRKAVEGMHGLPVTIRTVDVGS
HHCCCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCC
DKPLDRIDKAQDSHLNPALGLRAIRWSLADPPMFLTQLRAILRAAAHGQVNLLVPMLAHG
CCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
TEIRQTMAMIDHARATLDNKGTPYGPVRLGAMIEIPAAALSLKLFLKYFDFLSIGTNDLI
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECEEEECCHHHHHHHHHHHHHHHHHCCCHHHE
QYTLAIDRADESVAHLYDPCHPAVLRLVADTIAECNAQGKGVSVCGEMAGDVSMTRLLLG
EEHHEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHH
LGLRSFSMHPSRILAVKQQILRADAGKLSVWAAQVLDAEDPAALINPASAPMPSPVPANA
HCCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]