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The map label for this gene is fucA [C]

Identifier: 91786194

GI number: 91786194

Start: 299001

End: 299783

Strand: Direct

Name: fucA [C]

Synonym: Bpro_0283

Alternate gene names: 91786194

Gene position: 299001-299783 (Clockwise)

Preceding gene: 91786193

Following gene: 91786195

Centisome position: 5.75

GC content: 66.16

Gene sequence:

>783_bases
GTGAACGAGATCGTAGCCCGGCCGAAATCCGAGTACGACGCGAAGTTTCGCGCCGATCTGGACAAATTCGTGCAGGTGAG
CGGCTGGACGCTGCAGCAGAAGGTGGCGCTCGCGTGCCGCATTCTTGATCGCGATGGGCACGAATCCGCGCTCGCGGGCC
AGGTTTCGACGCGCGGGGAGAAGCCCGGCACCTACTGGACGCTGCGCTTCGGGCTTGGCTTCGACGAGGCCCGCGAGAGC
AACATCCTGCTGATCGACGACGACTTGAACGTCCTCGCGGGCGAGGGCATGGCCAACCCCGCGAATCGATTCCATCTGTG
GATCTATCGCGCCCGTCCCGAGACCCACGCGATCGTGCACACCCATCCGCCGTACGCCTCGGCGCTGTCGATGATCGCGG
AGGAACTCATCGTGTCGCACATGGACACCTGCGTGCTCTACGAGAATTGCGCTTATCTGCCCGAGTGGCCGGGCGTGCCG
ATCGGTGACGAGGAGGGCGAGATAATTTCGGCCGCGCTGGGCGACAAGCAGGCGGTGCTGCTCGCGCACCACGGCCTGCT
GACCGCGGCGAAGACGATCGAGGAGGCGGCGGTGCTCGCGTTCTACGTGGAGCGCGCGGCCAAGCTGCAACTCATGGCGC
GCGCGGTGGGGCCGATCAGGCGGGTGAAGCCGGAACTCGCGCGCGAGGCCCGTAGCTATCGCGGCAGTCCCAAGTACATC
GCTGCGACCTTCAATTACCTCGCGCGTCGCGTGCTGCGCGAGGCGCCGGACTGCCTGACATGA

Upstream 100 bases:

>100_bases
CGTCGCTGAGCGACGGACCTCGCGCAGCCGGGATGTTCGCGTTCGTCCGGTCGGGGCCGGGTGAGCTGAATACGGGGTCT
GCCAGAGAAAGGAGTCACCA

Downstream 100 bases:

>100_bases
ACCACCCCCGAAGCGCCTTCGGTGCCTCCCCCTCGAGGGGGCGACACCAGCGGACCGGCAAAGCCGGATCCGCGGTGTCC
GCTTGAAGCGCTCGTTTGAC

Product: class II aldolase/adducin domain-containing protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNEIVARPKSEYDAKFRADLDKFVQVSGWTLQQKVALACRILDRDGHESALAGQVSTRGEKPGTYWTLRFGLGFDEARES
NILLIDDDLNVLAGEGMANPANRFHLWIYRARPETHAIVHTHPPYASALSMIAEELIVSHMDTCVLYENCAYLPEWPGVP
IGDEEGEIISAALGDKQAVLLAHHGLLTAAKTIEEAAVLAFYVERAAKLQLMARAVGPIRRVKPELAREARSYRGSPKYI
AATFNYLARRVLREAPDCLT

Sequences:

>Translated_260_residues
MNEIVARPKSEYDAKFRADLDKFVQVSGWTLQQKVALACRILDRDGHESALAGQVSTRGEKPGTYWTLRFGLGFDEARES
NILLIDDDLNVLAGEGMANPANRFHLWIYRARPETHAIVHTHPPYASALSMIAEELIVSHMDTCVLYENCAYLPEWPGVP
IGDEEGEIISAALGDKQAVLLAHHGLLTAAKTIEEAAVLAFYVERAAKLQLMARAVGPIRRVKPELAREARSYRGSPKYI
AATFNYLARRVLREAPDCLT
>Mature_260_residues
MNEIVARPKSEYDAKFRADLDKFVQVSGWTLQQKVALACRILDRDGHESALAGQVSTRGEKPGTYWTLRFGLGFDEARES
NILLIDDDLNVLAGEGMANPANRFHLWIYRARPETHAIVHTHPPYASALSMIAEELIVSHMDTCVLYENCAYLPEWPGVP
IGDEEGEIISAALGDKQAVLLAHHGLLTAAKTIEEAAVLAFYVERAAKLQLMARAVGPIRRVKPELAREARSYRGSPKYI
AATFNYLARRVLREAPDCLT

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Escherichia coli, GI1789164, Length=187, Percent_Identity=29.9465240641711, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28914; Mature: 28914

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIVARPKSEYDAKFRADLDKFVQVSGWTLQQKVALACRILDRDGHESALAGQVSTRGE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHCCHHCCCC
KPGTYWTLRFGLGFDEARESNILLIDDDLNVLAGEGMANPANRFHLWIYRARPETHAIVH
CCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEEEEEEEECCCCEEEEE
THPPYASALSMIAEELIVSHMDTCVLYENCAYLPEWPGVPIGDEEGEIISAALGDKQAVL
CCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEE
LAHHGLLTAAKTIEEAAVLAFYVERAAKLQLMARAVGPIRRVKPELAREARSYRGSPKYI
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHCCCCCHH
AATFNYLARRVLREAPDCLT
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNEIVARPKSEYDAKFRADLDKFVQVSGWTLQQKVALACRILDRDGHESALAGQVSTRGE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHCCHHCCCC
KPGTYWTLRFGLGFDEARESNILLIDDDLNVLAGEGMANPANRFHLWIYRARPETHAIVH
CCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEEEEEEEECCCCEEEEE
THPPYASALSMIAEELIVSHMDTCVLYENCAYLPEWPGVPIGDEEGEIISAALGDKQAVL
CCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEE
LAHHGLLTAAKTIEEAAVLAFYVERAAKLQLMARAVGPIRRVKPELAREARSYRGSPKYI
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHCCCCCHH
AATFNYLARRVLREAPDCLT
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]