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The map label for this gene is yneJ [H]

Identifier: 91786193

GI number: 91786193

Start: 297820

End: 298701

Strand: Direct

Name: yneJ [H]

Synonym: Bpro_0282

Alternate gene names: 91786193

Gene position: 297820-298701 (Clockwise)

Preceding gene: 91786191

Following gene: 91786194

Centisome position: 5.73

GC content: 67.35

Gene sequence:

>882_bases
GTGCGATTCGACCTCACCACGCTAAGGCTCTTTGTCAGCGCGATCGAGCACGGCAGCATCGCAAGGGCCGCAGAGTGCGA
GCACATCGTCGCTTCGGCGGCGAGCAAACGCATCGGTGACCTCGAGACCGGGCTCGGAGTCGCGCTACTCGTACGCCAGC
ACGCGGGAGTCGTCCCGACCGTGGCGGGCGAAGCGCTCGCCCGCCACGCGCGCGAGGTCATCAAGACGCTCGACCGAATT
CCCGGGGCGGTCAGCACGCTTGCCGCGGACGATCAGCCCGATATCCGGGTGTTGGCAAATCAGACCGGCGTCGTCGTTGT
GGCCGCGGACCTCGCGAGTCACCTCGCGCGGCGGCCCGGGGTGAAGATTCGTCTCGGGGAAGGCCAGAGCCTGCCTATCA
TCGAAAGCGTCGCGCAAGGCAGCGCCGACATAGGCATCATCGGCCACTTTCAGCCCACCGACCGGCTGCACGTCGTTCCC
TATCGCAGCATTCCTCTCATGCTGGTCATGCCAGCGGCGCATCCGCTGGCCGGCCGCGGGGCGCTCTCATTCGCGGACGC
GCTCGAGTTCGATCTGATCACGCTGATGCAAGGCACCGCCATCCGCGGCTGGGCCCTGGCAGCAGCAGCCCGCATCGCGC
GCAAGCCGAAATTCACCATGCAGGTGCAGAGTTACGAGGCGATCCGCGCGATGGTGCAGGCGGGTCTCGGGATCGCGGTC
ATGCCCGCGGCCAACATTTTGCCGTACGAGGATCTCCTGCAGGTGCGCGCGCTGCCGCTCACCGACGATTGGGCGCGCAT
GCAGCTCTACCTGGTGTGCGACCGGGGTGCGGCCGGCATTCCTGCGATCGAGGAGCTGCTGGCGCATCTCGCCGCGGCTT
GA

Upstream 100 bases:

>100_bases
GTTACTCCTTCTCCCTTTGGGAGAAGGTTGGGATGAGGGTTGCCAGCGAGAGTAGTAGTATGCTTCTTCTCCTCCCGAAT
GCTCCAACACCCTACTATCC

Downstream 100 bases:

>100_bases
GCCTTCACGGCCGGCGATGGCAAGCGTTCCGAAAGATCATTTGCGAACGAAGCCGGATGGGTCCAAGCTAAGAGCCTGTT
CGCGATTTATTTGGGGACCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRFDLTTLRLFVSAIEHGSIARAAECEHIVASAASKRIGDLETGLGVALLVRQHAGVVPTVAGEALARHAREVIKTLDRI
PGAVSTLAADDQPDIRVLANQTGVVVVAADLASHLARRPGVKIRLGEGQSLPIIESVAQGSADIGIIGHFQPTDRLHVVP
YRSIPLMLVMPAAHPLAGRGALSFADALEFDLITLMQGTAIRGWALAAAARIARKPKFTMQVQSYEAIRAMVQAGLGIAV
MPAANILPYEDLLQVRALPLTDDWARMQLYLVCDRGAAGIPAIEELLAHLAAA

Sequences:

>Translated_293_residues
MRFDLTTLRLFVSAIEHGSIARAAECEHIVASAASKRIGDLETGLGVALLVRQHAGVVPTVAGEALARHAREVIKTLDRI
PGAVSTLAADDQPDIRVLANQTGVVVVAADLASHLARRPGVKIRLGEGQSLPIIESVAQGSADIGIIGHFQPTDRLHVVP
YRSIPLMLVMPAAHPLAGRGALSFADALEFDLITLMQGTAIRGWALAAAARIARKPKFTMQVQSYEAIRAMVQAGLGIAV
MPAANILPYEDLLQVRALPLTDDWARMQLYLVCDRGAAGIPAIEELLAHLAAA
>Mature_293_residues
MRFDLTTLRLFVSAIEHGSIARAAECEHIVASAASKRIGDLETGLGVALLVRQHAGVVPTVAGEALARHAREVIKTLDRI
PGAVSTLAADDQPDIRVLANQTGVVVVAADLASHLARRPGVKIRLGEGQSLPIIESVAQGSADIGIIGHFQPTDRLHVVP
YRSIPLMLVMPAAHPLAGRGALSFADALEFDLITLMQGTAIRGWALAAAARIARKPKFTMQVQSYEAIRAMVQAGLGIAV
MPAANILPYEDLLQVRALPLTDDWARMQLYLVCDRGAAGIPAIEELLAHLAAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=280, Percent_Identity=27.5, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=24.8299319727891, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=25.2525252525253, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=24.9027237354086, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790399, Length=281, Percent_Identity=26.3345195729537, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786713, Length=180, Percent_Identity=30, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=26.2295081967213, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=240, Percent_Identity=25, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=23.7704918032787, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31089; Mature: 31089

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLTTLRLFVSAIEHGSIARAAECEHIVASAASKRIGDLETGLGVALLVRQHAGVVPT
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHCCCCCH
VAGEALARHAREVIKTLDRIPGAVSTLAADDQPDIRVLANQTGVVVVAADLASHLARRPG
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCC
VKIRLGEGQSLPIIESVAQGSADIGIIGHFQPTDRLHVVPYRSIPLMLVMPAAHPLAGRG
CEEEECCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCC
ALSFADALEFDLITLMQGTAIRGWALAAAARIARKPKFTMQVQSYEAIRAMVQAGLGIAV
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCEEE
MPAANILPYEDLLQVRALPLTDDWARMQLYLVCDRGAAGIPAIEELLAHLAAA
ECCCCCCCHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRFDLTTLRLFVSAIEHGSIARAAECEHIVASAASKRIGDLETGLGVALLVRQHAGVVPT
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHCCCCCH
VAGEALARHAREVIKTLDRIPGAVSTLAADDQPDIRVLANQTGVVVVAADLASHLARRPG
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCC
VKIRLGEGQSLPIIESVAQGSADIGIIGHFQPTDRLHVVPYRSIPLMLVMPAAHPLAGRG
CEEEECCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCC
ALSFADALEFDLITLMQGTAIRGWALAAAARIARKPKFTMQVQSYEAIRAMVQAGLGIAV
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCEEE
MPAANILPYEDLLQVRALPLTDDWARMQLYLVCDRGAAGIPAIEELLAHLAAA
ECCCCCCCHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]