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The map label for this gene is pyrE [H]

Identifier: 91786128

GI number: 91786128

Start: 237276

End: 238031

Strand: Direct

Name: pyrE [H]

Synonym: Bpro_0217

Alternate gene names: 91786128

Gene position: 237276-238031 (Clockwise)

Preceding gene: 91786124

Following gene: 91786129

Centisome position: 4.56

GC content: 62.96

Gene sequence:

>756_bases
ATGAGCACGGCAGGGCAGACAGACAAAGCGGACAAGGCGCAGGCAACTCCCGCAGACACCACGCTGGCACAGGAATTCGT
GCAGTTTGCGCTGGAATCGGGTGTGCTGCGGTTTGGCGAATTCAAGACCAAGGCCGGGCGCCTGAGCCCCTATTTTTTCA
ACGCGGGCCTGTTTGACGACGGCGCCAAGCTGGGCCGCCTGGCGCAATTTTATGCGCGCCACCTGCTGGCCGAGGCCCAG
CGCAGCGGGCTGGAGTTTGACATGATTTTCGGCCCCGCGTACAAGGGCATTCCGCTGGCCGCCGCCGTCGCCGTCGAACT
GGCGCGGCAGGGCCGCAATGTCCCCTTTGCCTACAACCGCAAGGAGGCCAAGGACCATGGCGAAGGAGGCACGCTGGTCG
GGGCGCCCGTCAAGGGGCGCGTGCTGATCATTGATGACGTGATGTCAGCCGGCACGGCGGCGCGCGAATCGATTGCCATC
ATCCAGGCCGCCGGCGCCACACCCCATGCGATGGTGCTCGCGCTGGACCGGCAGGAAAAAGCCACCGAGAATGGCGTGGA
TGTTAACCACAGCGCGGTGCAATATGTAACGAATCAGCTGGGTTTGCAGGTTTGTGCGATTGCGCGCCTGACCGACTTGC
TGCAGTATCTCTCCAGGCAAGGCGACGTGTCGACGCCAGGGGCCTCTGGCGCGTTGTCCTTAAAAGCCCACTATCAGGCT
GTGCTGGCTTATCGGGAACGCTATGGCGTGGAATGA

Upstream 100 bases:

>100_bases
TCCGGTAGGGACTGGGCAATCCAGTCCTCCAGGCCTTTGCTGGTAGCGGAACGGATACCGTTGAGGTTGAGGCTGGTTAA
TTTGAACAAGGACATCTCCA

Downstream 100 bases:

>100_bases
GGAGTCGAATTGCTTTTGCAAACAGCGTTGACGGCGGGGCTGGTGCTGGCCACCTGCCTGTGCGGTGAGGCCGCAGCCCA
GAGCATCTACACCTGTGTCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSTAGQTDKADKAQATPADTTLAQEFVQFALESGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRLAQFYARHLLAEAQ
RSGLEFDMIFGPAYKGIPLAAAVAVELARQGRNVPFAYNRKEAKDHGEGGTLVGAPVKGRVLIIDDVMSAGTAARESIAI
IQAAGATPHAMVLALDRQEKATENGVDVNHSAVQYVTNQLGLQVCAIARLTDLLQYLSRQGDVSTPGASGALSLKAHYQA
VLAYRERYGVE

Sequences:

>Translated_251_residues
MSTAGQTDKADKAQATPADTTLAQEFVQFALESGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRLAQFYARHLLAEAQ
RSGLEFDMIFGPAYKGIPLAAAVAVELARQGRNVPFAYNRKEAKDHGEGGTLVGAPVKGRVLIIDDVMSAGTAARESIAI
IQAAGATPHAMVLALDRQEKATENGVDVNHSAVQYVTNQLGLQVCAIARLTDLLQYLSRQGDVSTPGASGALSLKAHYQA
VLAYRERYGVE
>Mature_250_residues
STAGQTDKADKAQATPADTTLAQEFVQFALESGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRLAQFYARHLLAEAQR
SGLEFDMIFGPAYKGIPLAAAVAVELARQGRNVPFAYNRKEAKDHGEGGTLVGAPVKGRVLIIDDVMSAGTAARESIAII
QAAGATPHAMVLALDRQEKATENGVDVNHSAVQYVTNQLGLQVCAIARLTDLLQYLSRQGDVSTPGASGALSLKAHYQAV
LAYRERYGVE

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=190, Percent_Identity=25.2631578947368, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790073, Length=228, Percent_Identity=48.2456140350877, Blast_Score=207, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17508631, Length=185, Percent_Identity=29.1891891891892, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=199, Percent_Identity=48.7437185929648, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323927, Length=242, Percent_Identity=43.3884297520661, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 26805; Mature: 26673

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAGQTDKADKAQATPADTTLAQEFVQFALESGVLRFGEFKTKAGRLSPYFFNAGLFDD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCCC
GAKLGRLAQFYARHLLAEAQRSGLEFDMIFGPAYKGIPLAAAVAVELARQGRNVPFAYNR
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEECC
KEAKDHGEGGTLVGAPVKGRVLIIDDVMSAGTAARESIAIIQAAGATPHAMVLALDRQEK
CHHHCCCCCCCEEECCCCCCEEEEECHHHCCHHHHHHEEEEEECCCCCCEEEEEECCHHH
ATENGVDVNHSAVQYVTNQLGLQVCAIARLTDLLQYLSRQGDVSTPGASGALSLKAHYQA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
VLAYRERYGVE
HHHHHHHHCCC
>Mature Secondary Structure 
STAGQTDKADKAQATPADTTLAQEFVQFALESGVLRFGEFKTKAGRLSPYFFNAGLFDD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCCC
GAKLGRLAQFYARHLLAEAQRSGLEFDMIFGPAYKGIPLAAAVAVELARQGRNVPFAYNR
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEECC
KEAKDHGEGGTLVGAPVKGRVLIIDDVMSAGTAARESIAIIQAAGATPHAMVLALDRQEK
CHHHCCCCCCCEEECCCCCCEEEEECHHHCCHHHHHHEEEEEECCCCCCEEEEEECCHHH
ATENGVDVNHSAVQYVTNQLGLQVCAIARLTDLLQYLSRQGDVSTPGASGALSLKAHYQA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
VLAYRERYGVE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA