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The map label for this gene is glcF [H]

Identifier: 91786099

GI number: 91786099

Start: 206519

End: 207751

Strand: Direct

Name: glcF [H]

Synonym: Bpro_0188

Alternate gene names: 91786099

Gene position: 206519-207751 (Clockwise)

Preceding gene: 91786098

Following gene: 91786104

Centisome position: 3.97

GC content: 67.15

Gene sequence:

>1233_bases
ATGCAAACCAACCTTGCCCCCGAGTTCAAAAACACCGCCGAAGGCCAGCAGGCCGAGGCAATATTGCGCAAGTGCGTGCA
TTGCGGGTTCTGCACCGCCACCTGCCCCACCTACCAGCTGCTGGGCGACGAGCTGGACGGCCCGCGCGGACGCATCTACC
TGATCAAGCAGGTGCTGGAGGGCGAGGTGCCGACCCGCAAGACCCAGCTGCACCTGGATCGCTGCCTGACCTGCCGCAAC
TGCGAAACCACCTGTCCGAGCGGCGTGCAGTACGGCCACCTGGTGGACATCGGCCGCAAGCTGGTCGACGAACGCGTGCC
GCGCCCGGCGGCCGAGGGCGCCATGCGATGGGCGCTCAAGGAAGGCCTGCCCTCACCGCTGTTTGCGCCCGCGATGAAGC
TGGGTCAGGCCGTGCGCGGGCTGCTGCCGGCGTCGCTCAAAAACAAGGTGCCTGCGCGGCAGGCCGCAGGGGTGTGGCCA
CAACGCGAACTTGCCCGCAAGGTGCTGATGCTGGAAGGCTGCGTGCAACCGGCCATGATGCCCAACATCAATGCGGCGAC
GGCGCGCGTGCTGGACGCGGCCGGCATCCAGGCGGTGGTGGCCCGCAACGCCGGTTGCTGCGGCGCGGTCAAGTTCCACC
TGAACGACCAGGAGGGCGGCAAGGCCGAGATGCGTCGCAACATCGACGCCTGGTGGCCGCATGTGCAAGCGGATGAGGGC
CAGGGGGTCGAGGCCATCGTCATGAACGCGTCGGGTTGCGGCGTCACCGTCAAGGAGTACGGCCACCATCTGCAGGACGA
CCCGGCCTATGCCGCGAAAGCGGCGCGCATCAGTGAATTGACGCGTGACCTCAGTGAGCTTTTGCCCGAGCTGGCCGACC
GGTTGCGCGGCCGGGTCTCGGCCCCGGCCGCACCAATGGTGTTTCACCCGCCCTGCACGCTGCAGCATGGCCAGCAGTTG
CGCGGCGGCGTGGAAAAGTACCTGGGCGAGCTGGGTTTCAACGTCACGGTGGCACGCAACGAAGCGCACCTGTGCTGCGG
CTCGGCGGGGACGTACTCGGTGCTCAATCCCGGCATTGCCTTTCAGCTGCGCGACCGCAAGCTGGAGAATTTGAACGAAA
TGGCGCCCGAGGTGATTGTGTCGGCCAACATCGGCTGCATCACGCACCTGCAAAGCGGCACGGCCACGCCGGTCCGGCAC
TGGGTGGAGGTGCTTGACGACGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
TTGCCGTCCCGCTGACCGTGCCAGTGCTGGCGTTCGCCATGGGCTTTCACCGCACCCTGCGGCGCAAGGTCAAAGCCATT
CAACCGTCTTCCTGAACACC

Downstream 100 bases:

>100_bases
TCGCTACGCTTTGAGCGGATACCGAGGCCGATTCCGGACGGCTTCGCGCTACTGCTGTTGTTGCTGTTGCTGCGACCTCG
CCAGCAGTACCGCACGGCGC

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQTNLAPEFKNTAEGQQAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGEVPTRKTQLHLDRCLTCRN
CETTCPSGVQYGHLVDIGRKLVDERVPRPAAEGAMRWALKEGLPSPLFAPAMKLGQAVRGLLPASLKNKVPARQAAGVWP
QRELARKVLMLEGCVQPAMMPNINAATARVLDAAGIQAVVARNAGCCGAVKFHLNDQEGGKAEMRRNIDAWWPHVQADEG
QGVEAIVMNASGCGVTVKEYGHHLQDDPAYAAKAARISELTRDLSELLPELADRLRGRVSAPAAPMVFHPPCTLQHGQQL
RGGVEKYLGELGFNVTVARNEAHLCCGSAGTYSVLNPGIAFQLRDRKLENLNEMAPEVIVSANIGCITHLQSGTATPVRH
WVEVLDDALA

Sequences:

>Translated_410_residues
MQTNLAPEFKNTAEGQQAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGEVPTRKTQLHLDRCLTCRN
CETTCPSGVQYGHLVDIGRKLVDERVPRPAAEGAMRWALKEGLPSPLFAPAMKLGQAVRGLLPASLKNKVPARQAAGVWP
QRELARKVLMLEGCVQPAMMPNINAATARVLDAAGIQAVVARNAGCCGAVKFHLNDQEGGKAEMRRNIDAWWPHVQADEG
QGVEAIVMNASGCGVTVKEYGHHLQDDPAYAAKAARISELTRDLSELLPELADRLRGRVSAPAAPMVFHPPCTLQHGQQL
RGGVEKYLGELGFNVTVARNEAHLCCGSAGTYSVLNPGIAFQLRDRKLENLNEMAPEVIVSANIGCITHLQSGTATPVRH
WVEVLDDALA
>Mature_410_residues
MQTNLAPEFKNTAEGQQAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGEVPTRKTQLHLDRCLTCRN
CETTCPSGVQYGHLVDIGRKLVDERVPRPAAEGAMRWALKEGLPSPLFAPAMKLGQAVRGLLPASLKNKVPARQAAGVWP
QRELARKVLMLEGCVQPAMMPNINAATARVLDAAGIQAVVARNAGCCGAVKFHLNDQEGGKAEMRRNIDAWWPHVQADEG
QGVEAIVMNASGCGVTVKEYGHHLQDDPAYAAKAARISELTRDLSELLPELADRLRGRVSAPAAPMVFHPPCTLQHGQQL
RGGVEKYLGELGFNVTVARNEAHLCCGSAGTYSVLNPGIAFQLRDRKLENLNEMAPEVIVSANIGCITHLQSGTATPVRH
WVEVLDDALA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=411, Percent_Identity=55.9610705596107, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1786497, Length=243, Percent_Identity=23.0452674897119, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788576, Length=419, Percent_Identity=22.9116945107399, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 44422; Mature: 44422

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLAPEFKNTAEGQQAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GEVPTRKTQLHLDRCLTCRNCETTCPSGVQYGHLVDIGRKLVDERVPRPAAEGAMRWALK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EGLPSPLFAPAMKLGQAVRGLLPASLKNKVPARQAAGVWPQRELARKVLMLEGCVQPAMM
HCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCC
PNINAATARVLDAAGIQAVVARNAGCCGAVKFHLNDQEGGKAEMRRNIDAWWPHVQADEG
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHCCCHHHCCCCCCCCC
QGVEAIVMNASGCGVTVKEYGHHLQDDPAYAAKAARISELTRDLSELLPELADRLRGRVS
CCCEEEEEECCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APAAPMVFHPPCTLQHGQQLRGGVEKYLGELGFNVTVARNEAHLCCGSAGTYSVLNPGIA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCHHCCCCEE
FQLRDRKLENLNEMAPEVIVSANIGCITHLQSGTATPVRHWVEVLDDALA
EEECHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLAPEFKNTAEGQQAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GEVPTRKTQLHLDRCLTCRNCETTCPSGVQYGHLVDIGRKLVDERVPRPAAEGAMRWALK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EGLPSPLFAPAMKLGQAVRGLLPASLKNKVPARQAAGVWPQRELARKVLMLEGCVQPAMM
HCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCC
PNINAATARVLDAAGIQAVVARNAGCCGAVKFHLNDQEGGKAEMRRNIDAWWPHVQADEG
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHCCCHHHCCCCCCCCC
QGVEAIVMNASGCGVTVKEYGHHLQDDPAYAAKAARISELTRDLSELLPELADRLRGRVS
CCCEEEEEECCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APAAPMVFHPPCTLQHGQQLRGGVEKYLGELGFNVTVARNEAHLCCGSAGTYSVLNPGIA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCHHCCCCEE
FQLRDRKLENLNEMAPEVIVSANIGCITHLQSGTATPVRHWVEVLDDALA
EEECHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]