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The map label for this gene is metH [H]

Identifier: 91786083

GI number: 91786083

Start: 186740

End: 187786

Strand: Direct

Name: metH [H]

Synonym: Bpro_0172

Alternate gene names: 91786083

Gene position: 186740-187786 (Clockwise)

Preceding gene: 91786077

Following gene: 91786085

Centisome position: 3.59

GC content: 64.28

Gene sequence:

>1047_bases
ATGAAGCCCCTAAATTACACCCGCGGACAGCAACTGCCAGCCATCCTGGAACAACGCATCGCCATCCTTGACGGCGCGAT
GGGCACGATGATCCAGCGCTTCAAGTTGAACGAAGCCCAGTACCGCGGCGAGCGCTTCAAGGACTTCCACAAGGACGTCA
AGGGCAACAACGAACTGCTCAGCCTGACCCGTCCCGACGTGATCCGCGACATCCATGAAGGCTACCTCGCCGCCGGCGCC
GACATGATAGAGACCAACACCTTTGGTGCCACCACGGTCGCCCAGGCCGACTACGACATGGCGGATCTGGCCGTGGAGAT
GAACTACGAGTCGGCCCGCATCGCGCGTGCCGCCTGCGACAAGTTTTCCACGCCCGACAAGCCGCGCTTCGTGGTGGGCG
CCCTGGGCCCGACGCCCAAAACCGCCAGCATCAGCCCCGATGTGAACGACCCGGGCGCGCGCAACACCAGCTTTGAGGAG
CTGCGCAAGGCTTATTACGAGCAGACGGAAGCGCTGGTCAAGGGCGGCAGCGATGTGCTGCTGGTCGAAACCATTTTCGA
CACACTCAACGCCAAGGCGGCCCTGTTTGCGATCGACGAGTACTTCGACAACTCCGGTGAGTGCCTGCCCCTCATCATCA
GCGGCACCGTCACGGACGCGTCGGGCCGCATCCTGAGCGGCCAGACCGTGACCGCCTTCTGGCACAGCGTGCGCCATGCC
CGGCCGCTGGCGATTGGTCTCAATTGCGCGCTGGGCGCCACGCTGATGCGCCCCTACATCCAGGAACTGGCCCGTGTGGC
CGGCGACACCTTTATCAGCTGCTACCCGAATGCCGGACTGCCCAACCCCATGAGCGACACCGGCTTCGATGAAACGCCCG
ACGTGACCTCGCGCCTGCTGCGCGAATTTGCCGACGAAGGGCTGGTCAACATCGTGGGCGGCTGCTGCGGCACCACGCCA
GAGCATATCCAGGCCATTGCGCATGCGGTGGCGCCCCTGCCGTCCCGGGCTGTCGCTTCCCGCTTCTTCTACCGTGAGGC
GGCTTAG

Upstream 100 bases:

>100_bases
CTGGTGCAGAGCTCTTGCGAAGGGTAGGGCGGCTCTGGCGAAAAACAGGGACGCTATTCAGGAGCCTGCTGAAAAAACCG
CCGCACCTTAAAATCGGCGC

Downstream 100 bases:

>100_bases
GCCGGGTTAGATCGCGGGTCAGGCCGGGTTGGCGGGCACCGCGCTTTTTTATTCGCGTGAGGCGTCAGTCCGGCAGCAGG
TGATCGATTTCCTGCTGCAT

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKPLNYTRGQQLPAILEQRIAILDGAMGTMIQRFKLNEAQYRGERFKDFHKDVKGNNELLSLTRPDVIRDIHEGYLAAGA
DMIETNTFGATTVAQADYDMADLAVEMNYESARIARAACDKFSTPDKPRFVVGALGPTPKTASISPDVNDPGARNTSFEE
LRKAYYEQTEALVKGGSDVLLVETIFDTLNAKAALFAIDEYFDNSGECLPLIISGTVTDASGRILSGQTVTAFWHSVRHA
RPLAIGLNCALGATLMRPYIQELARVAGDTFISCYPNAGLPNPMSDTGFDETPDVTSRLLREFADEGLVNIVGGCCGTTP
EHIQAIAHAVAPLPSRAVASRFFYREAA

Sequences:

>Translated_348_residues
MKPLNYTRGQQLPAILEQRIAILDGAMGTMIQRFKLNEAQYRGERFKDFHKDVKGNNELLSLTRPDVIRDIHEGYLAAGA
DMIETNTFGATTVAQADYDMADLAVEMNYESARIARAACDKFSTPDKPRFVVGALGPTPKTASISPDVNDPGARNTSFEE
LRKAYYEQTEALVKGGSDVLLVETIFDTLNAKAALFAIDEYFDNSGECLPLIISGTVTDASGRILSGQTVTAFWHSVRHA
RPLAIGLNCALGATLMRPYIQELARVAGDTFISCYPNAGLPNPMSDTGFDETPDVTSRLLREFADEGLVNIVGGCCGTTP
EHIQAIAHAVAPLPSRAVASRFFYREAA
>Mature_348_residues
MKPLNYTRGQQLPAILEQRIAILDGAMGTMIQRFKLNEAQYRGERFKDFHKDVKGNNELLSLTRPDVIRDIHEGYLAAGA
DMIETNTFGATTVAQADYDMADLAVEMNYESARIARAACDKFSTPDKPRFVVGALGPTPKTASISPDVNDPGARNTSFEE
LRKAYYEQTEALVKGGSDVLLVETIFDTLNAKAALFAIDEYFDNSGECLPLIISGTVTDASGRILSGQTVTAFWHSVRHA
RPLAIGLNCALGATLMRPYIQELARVAGDTFISCYPNAGLPNPMSDTGFDETPDVTSRLLREFADEGLVNIVGGCCGTTP
EHIQAIAHAVAPLPSRAVASRFFYREAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=334, Percent_Identity=54.4910179640719, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI1790450, Length=343, Percent_Identity=55.3935860058309, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=54.7112462006079, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24585077, Length=285, Percent_Identity=25.2631578947368, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37863; Mature: 37863

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLNYTRGQQLPAILEQRIAILDGAMGTMIQRFKLNEAQYRGERFKDFHKDVKGNNELL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEE
SLTRPDVIRDIHEGYLAAGADMIETNTFGATTVAQADYDMADLAVEMNYESARIARAACD
ECCCHHHHHHHHHHHHHCCCCHHCCCCCCCCEEECCCCCHHHEEEECCCCHHHHHHHHHH
KFSTPDKPRFVVGALGPTPKTASISPDVNDPGARNTSFEELRKAYYEQTEALVKGGSDVL
HCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
LVETIFDTLNAKAALFAIDEYFDNSGECLPLIISGTVTDASGRILSGQTVTAFWHSVRHA
EHHHHHHHHCCCHHEEEHHHHHCCCCCEEEEEEEEEEECCCCCEECCCHHHHHHHHHHHC
RPLAIGLNCALGATLMRPYIQELARVAGDTFISCYPNAGLPNPMSDTGFDETPDVTSRLL
CCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
REFADEGLVNIVGGCCGTTPEHIQAIAHAVAPLPSRAVASRFFYREAA
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPLNYTRGQQLPAILEQRIAILDGAMGTMIQRFKLNEAQYRGERFKDFHKDVKGNNELL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEE
SLTRPDVIRDIHEGYLAAGADMIETNTFGATTVAQADYDMADLAVEMNYESARIARAACD
ECCCHHHHHHHHHHHHHCCCCHHCCCCCCCCEEECCCCCHHHEEEECCCCHHHHHHHHHH
KFSTPDKPRFVVGALGPTPKTASISPDVNDPGARNTSFEELRKAYYEQTEALVKGGSDVL
HCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
LVETIFDTLNAKAALFAIDEYFDNSGECLPLIISGTVTDASGRILSGQTVTAFWHSVRHA
EHHHHHHHHCCCHHEEEHHHHHCCCCCEEEEEEEEEEECCCCCEECCCHHHHHHHHHHHC
RPLAIGLNCALGATLMRPYIQELARVAGDTFISCYPNAGLPNPMSDTGFDETPDVTSRLL
CCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
REFADEGLVNIVGGCCGTTPEHIQAIAHAVAPLPSRAVASRFFYREAA
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]