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The map label for this gene is iaaA [H]

Identifier: 91786052

GI number: 91786052

Start: 148605

End: 149591

Strand: Reverse

Name: iaaA [H]

Synonym: Bpro_0141

Alternate gene names: 91786052

Gene position: 149591-148605 (Counterclockwise)

Preceding gene: 91786053

Following gene: 91786051

Centisome position: 2.88

GC content: 69.91

Gene sequence:

>987_bases
GTGACACCCTCCAAGCCCCTGCCCGTCATCGCCATCCACGGTGGCGCCGGCACCATGAGCCGCAACCACATCAGCGCCGA
GCAAACCGCGGCTTACCACGCTGCCCTGCAGGGCATCCTGCGGGTCGCGCAGCAATTGCTGGCCGATGGCGGCAGCGCGC
TCGATGCCGTCAGCCTGGCCGTTGACCTGCTGGAAGACTGCCCGCTCTTCAATGCCGGCCATGGCTCTGTCTTCACCCAT
GATGAAACCCATGAGCTGGACGCTGCCGTGATGGACGGCGCCACCCTGCGCGCCGGCGCGGTGGCTTGCGTGAGCCGCGT
GCGCCGCCCCTTGCGCGCCGCCCGCGCCGTGATGGAGCACAGCGAGCATGTGCTGCTGGTGGGCGCCGGCGCCGAAGCCT
TCGCCGAAGGCCTGGGGATGGAGATGGTTTCGCCCGATTACTTTTCAACCGAGGCCCGCCGCGAGCAATTGCGCCGCGCC
CTGTCCACCGACCAGGCCATGCTGGACCATGACGGCGCGGCCCTGGTGTTCCGTCCCTCGGCAGCCGGCGCGGCGCCGCT
GGATGATGACCGCAAATTCGGCACCGTGGGCGCTGTCGCGCTCGATGCACACGGCAACCTGGCCGCCGCCACCTCCACCG
GCGGCATGACCAACAAACGGCCCGGCCGCGTCGGCGACACGCCCCTGATCGGCGCCGGCACCTATGCCGACAATCGCACG
GCGGCCGTCTCCTGCACCGGCACCGGCGAAATGTTCATACGCACCGTGGCGGCCTACGACCTCTGCGCGCGCATGGCCTA
CGGCGGCCTGTCGCTGGACGCGGCGGCGCAGCAGGTGGTGATGGACGTGCTGCCCGCCATGGGCGGCCGGGGCGGCCTGA
TTGCCGTCGACGTGCAGGGCAAGCTGAGCCTTCCCTTCAACACCGAAGGCATGTACCGCGGCCATGCCCGCGTCGGTGAA
GCGCCGCAGACCGCGATTTTTCGCTGA

Upstream 100 bases:

>100_bases
TGCACGGCAGCCACAGCGGCCGGCTGCGCGCCGTGGCCGAGGCCGCGGCGCCTGCGCCCCGCAAGGCCAACGCCAAATCG
AAATCCTCAAAGGCCCCCAA

Downstream 100 bases:

>100_bases
TCGCCATCTGCGGCACGCTCCTTGCTGTTTCCACAAACATCTCACACGCATCCACCCCAAAAGCGCACCCCAATGCAACC
CACCGTGGCCGAAGCCTCTC

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTPSKPLPVIAIHGGAGTMSRNHISAEQTAAYHAALQGILRVAQQLLADGGSALDAVSLAVDLLEDCPLFNAGHGSVFTH
DETHELDAAVMDGATLRAGAVACVSRVRRPLRAARAVMEHSEHVLLVGAGAEAFAEGLGMEMVSPDYFSTEARREQLRRA
LSTDQAMLDHDGAALVFRPSAAGAAPLDDDRKFGTVGAVALDAHGNLAAATSTGGMTNKRPGRVGDTPLIGAGTYADNRT
AAVSCTGTGEMFIRTVAAYDLCARMAYGGLSLDAAAQQVVMDVLPAMGGRGGLIAVDVQGKLSLPFNTEGMYRGHARVGE
APQTAIFR

Sequences:

>Translated_328_residues
MTPSKPLPVIAIHGGAGTMSRNHISAEQTAAYHAALQGILRVAQQLLADGGSALDAVSLAVDLLEDCPLFNAGHGSVFTH
DETHELDAAVMDGATLRAGAVACVSRVRRPLRAARAVMEHSEHVLLVGAGAEAFAEGLGMEMVSPDYFSTEARREQLRRA
LSTDQAMLDHDGAALVFRPSAAGAAPLDDDRKFGTVGAVALDAHGNLAAATSTGGMTNKRPGRVGDTPLIGAGTYADNRT
AAVSCTGTGEMFIRTVAAYDLCARMAYGGLSLDAAAQQVVMDVLPAMGGRGGLIAVDVQGKLSLPFNTEGMYRGHARVGE
APQTAIFR
>Mature_327_residues
TPSKPLPVIAIHGGAGTMSRNHISAEQTAAYHAALQGILRVAQQLLADGGSALDAVSLAVDLLEDCPLFNAGHGSVFTHD
ETHELDAAVMDGATLRAGAVACVSRVRRPLRAARAVMEHSEHVLLVGAGAEAFAEGLGMEMVSPDYFSTEARREQLRRAL
STDQAMLDHDGAALVFRPSAAGAAPLDDDRKFGTVGAVALDAHGNLAAATSTGGMTNKRPGRVGDTPLIGAGTYADNRTA
AVSCTGTGEMFIRTVAAYDLCARMAYGGLSLDAAAQQVVMDVLPAMGGRGGLIAVDVQGKLSLPFNTEGMYRGHARVGEA
PQTAIFR

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=316, Percent_Identity=37.3417721518987, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI145275200, Length=316, Percent_Identity=37.3417721518987, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI98991769, Length=267, Percent_Identity=30.7116104868914, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI285002251, Length=241, Percent_Identity=33.195020746888, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI285002253, Length=241, Percent_Identity=31.1203319502075, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787050, Length=320, Percent_Identity=60.9375, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32564649, Length=328, Percent_Identity=26.219512195122, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18921183, Length=320, Percent_Identity=39.6875, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21357893, Length=281, Percent_Identity=33.4519572953737, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652135, Length=235, Percent_Identity=32.7659574468085, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI221330102, Length=235, Percent_Identity=32.7659574468085, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33976; Mature: 33845

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSKPLPVIAIHGGAGTMSRNHISAEQTAAYHAALQGILRVAQQLLADGGSALDAVSLA
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VDLLEDCPLFNAGHGSVFTHDETHELDAAVMDGATLRAGAVACVSRVRRPLRAARAVMEH
HHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SEHVLLVGAGAEAFAEGLGMEMVSPDYFSTEARREQLRRALSTDQAMLDHDGAALVFRPS
CCEEEEEECCHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHHCCCHHHHCCCCCEEEEECC
AAGAAPLDDDRKFGTVGAVALDAHGNLAAATSTGGMTNKRPGRVGDTPLIGAGTYADNRT
CCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCE
AAVSCTGTGEMFIRTVAAYDLCARMAYGGLSLDAAAQQVVMDVLPAMGGRGGLIAVDVQG
EEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
KLSLPFNTEGMYRGHARVGEAPQTAIFR
EEECCCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TPSKPLPVIAIHGGAGTMSRNHISAEQTAAYHAALQGILRVAQQLLADGGSALDAVSLA
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VDLLEDCPLFNAGHGSVFTHDETHELDAAVMDGATLRAGAVACVSRVRRPLRAARAVMEH
HHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SEHVLLVGAGAEAFAEGLGMEMVSPDYFSTEARREQLRRALSTDQAMLDHDGAALVFRPS
CCEEEEEECCHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHHCCCHHHHCCCCCEEEEECC
AAGAAPLDDDRKFGTVGAVALDAHGNLAAATSTGGMTNKRPGRVGDTPLIGAGTYADNRT
CCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCE
AAVSCTGTGEMFIRTVAAYDLCARMAYGGLSLDAAAQQVVMDVLPAMGGRGGLIAVDVQG
EEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
KLSLPFNTEGMYRGHARVGEAPQTAIFR
EEECCCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]