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The map label for this gene is lip2 [H]

Identifier: 91786045

GI number: 91786045

Start: 140163

End: 141122

Strand: Direct

Name: lip2 [H]

Synonym: Bpro_0134

Alternate gene names: 91786045

Gene position: 140163-141122 (Clockwise)

Preceding gene: 91786044

Following gene: 91786055

Centisome position: 2.7

GC content: 68.12

Gene sequence:

>960_bases
ATGACGACATCTCCTGTTCGCACCACGCTGGACGCTACGCCTCTCGACCCCGCCTTTGCCGGTTTTCTGGCCCAAGCTGC
GGCCCAGGGAGCCCCCCCTCTGGAGGCCTTGCCGCCTCCCATTGCGCGGCAGGTCTACCGGGACCTGGCCAACGCCTTGG
GCCTGCCCGCCCCGGCCATTGGCAAGGTGGAAGATAAAAGCATTCCCGGCCCCGGTGGCGTGCTGCCTTTGCGGGCATAC
AGTCCCACGACTCAGGGGCCGTGGCCCGTGCTGTTGTACGTGCATGGAGGAGGTTTTGTGATGGGCGATCTGCAAACGCA
TGACGAGGTCTGCCGCACCCTGTGCCACCTTACGGGCATGCTGGTCGTGGCGGTGGACTACCGGCTGGCTCCCGAGCATC
CGTTCCCGGCCGCCCCTGATGATGCGGAAGCGGCCTTGCGCTGGATCTGCGTCCATGCGGACACCCTGGGTGGTGACCCG
CATCGCATCGCCGTGGCGGGCGACAGCGCCGGGGCACAGCTGGCCCTGGTGGCGGTGCGGCGGGTGCAGTCCCCCGCGGT
GCGAGCCCTGGGCCTGATTTACCCCGTGGCGCAGCACCCGGACGAGCCCAGTCCGTCGTACCAGGAAAATGAAGGCAAGT
TCCTCACCAAGGGGGTCATGCAGTGGTTCACCGCCAGCTATCTGGGCGGGCCATCACCCACGCCGCCGGATCATCCCGAT
GTGGCGCTGCTGAAGTCCGAGCTGGCGGCGCTGCCCGCCACCTGGGTCGCCACCATGGGCCATGACCCGCTGCGTGACGA
AGGCCATGCGCTGGCCCTGCGTGCCGAGGCCGCAGGCGTTTTGGTCGCGCACCAGCACTACCCCAGCGCCATCCACGCCT
GCATCCATTTCAGCGCCGTGTCCCCCATTGGCCCGCAGGTGCTGGCGGACTTGGCGCAGTGGTTGCGCGAGCAGGCTTGA

Upstream 100 bases:

>100_bases
TGGTGCTGCTGCCTGCGTTGGGGCACTTTTTGCTGCGCAAACCAGTGGTGGCGGTGGTTGCGCCAGCCGCGATTTCTGTA
TCAATCTAATCTTTAAATCC

Downstream 100 bases:

>100_bases
TGTGCCAGGCCGACTGGCGTGGTCTGTGATGCGCTAGCGCAGCAGGGTTGACATGGCCGACAGGCAGTTGACCATGCGCA
CCGCAGCCTCCACCGTCGGT

Product: alpha/beta hydrolase fold-3 protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTTSPVRTTLDATPLDPAFAGFLAQAAAQGAPPLEALPPPIARQVYRDLANALGLPAPAIGKVEDKSIPGPGGVLPLRAY
SPTTQGPWPVLLYVHGGGFVMGDLQTHDEVCRTLCHLTGMLVVAVDYRLAPEHPFPAAPDDAEAALRWICVHADTLGGDP
HRIAVAGDSAGAQLALVAVRRVQSPAVRALGLIYPVAQHPDEPSPSYQENEGKFLTKGVMQWFTASYLGGPSPTPPDHPD
VALLKSELAALPATWVATMGHDPLRDEGHALALRAEAAGVLVAHQHYPSAIHACIHFSAVSPIGPQVLADLAQWLREQA

Sequences:

>Translated_319_residues
MTTSPVRTTLDATPLDPAFAGFLAQAAAQGAPPLEALPPPIARQVYRDLANALGLPAPAIGKVEDKSIPGPGGVLPLRAY
SPTTQGPWPVLLYVHGGGFVMGDLQTHDEVCRTLCHLTGMLVVAVDYRLAPEHPFPAAPDDAEAALRWICVHADTLGGDP
HRIAVAGDSAGAQLALVAVRRVQSPAVRALGLIYPVAQHPDEPSPSYQENEGKFLTKGVMQWFTASYLGGPSPTPPDHPD
VALLKSELAALPATWVATMGHDPLRDEGHALALRAEAAGVLVAHQHYPSAIHACIHFSAVSPIGPQVLADLAQWLREQA
>Mature_318_residues
TTSPVRTTLDATPLDPAFAGFLAQAAAQGAPPLEALPPPIARQVYRDLANALGLPAPAIGKVEDKSIPGPGGVLPLRAYS
PTTQGPWPVLLYVHGGGFVMGDLQTHDEVCRTLCHLTGMLVVAVDYRLAPEHPFPAAPDDAEAALRWICVHADTLGGDPH
RIAVAGDSAGAQLALVAVRRVQSPAVRALGLIYPVAQHPDEPSPSYQENEGKFLTKGVMQWFTASYLGGPSPTPPDHPDV
ALLKSELAALPATWVATMGHDPLRDEGHALALRAEAAGVLVAHQHYPSAIHACIHFSAVSPIGPQVLADLAQWLREQA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=313, Percent_Identity=29.7124600638978, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI68299767, Length=293, Percent_Identity=27.3037542662116, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI157041239, Length=146, Percent_Identity=37.6712328767123, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI68051721, Length=300, Percent_Identity=25.3333333333333, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226423947, Length=308, Percent_Identity=24.6753246753247, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI61966717, Length=288, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI21328446, Length=99, Percent_Identity=35.3535353535354, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157041237, Length=278, Percent_Identity=24.8201438848921, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=263, Percent_Identity=29.277566539924, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=264, Percent_Identity=28.7878787878788, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540028, Length=174, Percent_Identity=29.8850574712644, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=167, Percent_Identity=31.1377245508982, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI72001146, Length=177, Percent_Identity=31.0734463276836, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24656084, Length=99, Percent_Identity=35.3535353535354, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656076, Length=99, Percent_Identity=35.3535353535354, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130169, Length=99, Percent_Identity=35.3535353535354, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33598; Mature: 33466

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSPVRTTLDATPLDPAFAGFLAQAAAQGAPPLEALPPPIARQVYRDLANALGLPAPAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
GKVEDKSIPGPGGVLPLRAYSPTTQGPWPVLLYVHGGGFVMGDLQTHDEVCRTLCHLTGM
CCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHHHH
LVVAVDYRLAPEHPFPAAPDDAEAALRWICVHADTLGGDPHRIAVAGDSAGAQLALVAVR
HHHEEECEECCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
RVQSPAVRALGLIYPVAQHPDEPSPSYQENEGKFLTKGVMQWFTASYLGGPSPTPPDHPD
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VALLKSELAALPATWVATMGHDPLRDEGHALALRAEAAGVLVAHQHYPSAIHACIHFSAV
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCC
SPIGPQVLADLAQWLREQA
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTSPVRTTLDATPLDPAFAGFLAQAAAQGAPPLEALPPPIARQVYRDLANALGLPAPAI
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
GKVEDKSIPGPGGVLPLRAYSPTTQGPWPVLLYVHGGGFVMGDLQTHDEVCRTLCHLTGM
CCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHHHH
LVVAVDYRLAPEHPFPAAPDDAEAALRWICVHADTLGGDPHRIAVAGDSAGAQLALVAVR
HHHEEECEECCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
RVQSPAVRALGLIYPVAQHPDEPSPSYQENEGKFLTKGVMQWFTASYLGGPSPTPPDHPD
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VALLKSELAALPATWVATMGHDPLRDEGHALALRAEAAGVLVAHQHYPSAIHACIHFSAV
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCC
SPIGPQVLADLAQWLREQA
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]