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The map label for this gene is ywbI [H]

Identifier: 91785983

GI number: 91785983

Start: 73407

End: 74297

Strand: Reverse

Name: ywbI [H]

Synonym: Bpro_0070

Alternate gene names: 91785983

Gene position: 74297-73407 (Counterclockwise)

Preceding gene: 91785984

Following gene: 91785982

Centisome position: 1.43

GC content: 69.47

Gene sequence:

>891_bases
ATGCGTGAACTCAATCTGGACCAGCTGCGCACCCTCGTTTCCATCGCCGACCTGGGCACCTTCTCCGCCGCGGCACGGGC
GCTGCATCTGGCGCAGCCCACGGTCAGCCTGCACATCAGCGAGCTGGAAGCCCGGCTGGGCGCACAGCTGGTGGTGCGCG
GGGGCCGCAGCGTGGTACCCACGGCGGCCGGTGCCGTGCTGGTCGAGCGGGCGCGGCGCCTGCTGCGCGACGCCGATGAC
GCGACCGATGCCGTCAAGCGCCATGTAGAGGGGCGGCTAGGCCGCGTGCGGCTGGGCACCTCCACCGGCATCGTGGTGCA
TTTGCTGCCCCGGGTGCTGGAGCTCATGGCGCGCGCGCATCCCGAGATCGATGTGGAGGTCAGCATCCTCGGCTCCGCTG
AAACCATGGAACGCCTGGGCCAGGGCACGCTGGATGTCGGATTAGTGGCTACGCCCCAGCCGGCGCGGCGCGAGCTGGTG
ATGAGCCACTGGCGCAGCGACCCCATGATGGCGTTCATCCCCACCGGCTGGCCGGCGCCCAAGCGCATCACGCCGCCCTG
GCTGATGGACAAGCCCCTGATATTCAACGACTCGACCACGCACATGTACCGCCTGACCATGGAGTGGTTTGCCAAGGCCG
GCCACACGCCCCGGGCGCGCATAGAGCTCAATTACACCGAGGCCATGAAGAGCCTGGTGGTGGCCGGCTATGGTGCGGCC
CTGCTGCCGCTGGAGCAGGCGGGCGCTGCCGCCACTCCCAACGGCGTGCAGGTGCTGCCGCTCAAGCCCGCATTGACCCG
CCACACCGCCGTGGTGCACAGGCCGCTGGCAGCCCTTGACGGGGCCACGCGCAACCTGCTGCAGACGCTGGCGCAGTTCC
GGCAAACTTGA

Upstream 100 bases:

>100_bases
AACATGGTGGCAACTCCTGTGATCGAGAAGTGCCCAATGGTGCGGCCTGTCGTGCTATTGTGGAAGTTGAATTTTCTGAT
GCAACCTATCAGGATTAATG

Downstream 100 bases:

>100_bases
AGCCCGACCCGGGCGGCAGCACGCCAAACCACGGCGTGGCCGACACGCGGAAAGCAGGCACGCTGATGAAAGTTCAATTT
CTGACAATTGCACGTGCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRELNLDQLRTLVSIADLGTFSAAARALHLAQPTVSLHISELEARLGAQLVVRGGRSVVPTAAGAVLVERARRLLRDADD
ATDAVKRHVEGRLGRVRLGTSTGIVVHLLPRVLELMARAHPEIDVEVSILGSAETMERLGQGTLDVGLVATPQPARRELV
MSHWRSDPMMAFIPTGWPAPKRITPPWLMDKPLIFNDSTTHMYRLTMEWFAKAGHTPRARIELNYTEAMKSLVVAGYGAA
LLPLEQAGAAATPNGVQVLPLKPALTRHTAVVHRPLAALDGATRNLLQTLAQFRQT

Sequences:

>Translated_296_residues
MRELNLDQLRTLVSIADLGTFSAAARALHLAQPTVSLHISELEARLGAQLVVRGGRSVVPTAAGAVLVERARRLLRDADD
ATDAVKRHVEGRLGRVRLGTSTGIVVHLLPRVLELMARAHPEIDVEVSILGSAETMERLGQGTLDVGLVATPQPARRELV
MSHWRSDPMMAFIPTGWPAPKRITPPWLMDKPLIFNDSTTHMYRLTMEWFAKAGHTPRARIELNYTEAMKSLVVAGYGAA
LLPLEQAGAAATPNGVQVLPLKPALTRHTAVVHRPLAALDGATRNLLQTLAQFRQT
>Mature_296_residues
MRELNLDQLRTLVSIADLGTFSAAARALHLAQPTVSLHISELEARLGAQLVVRGGRSVVPTAAGAVLVERARRLLRDADD
ATDAVKRHVEGRLGRVRLGTSTGIVVHLLPRVLELMARAHPEIDVEVSILGSAETMERLGQGTLDVGLVATPQPARRELV
MSHWRSDPMMAFIPTGWPAPKRITPPWLMDKPLIFNDSTTHMYRLTMEWFAKAGHTPRARIELNYTEAMKSLVVAGYGAA
LLPLEQAGAAATPNGVQVLPLKPALTRHTAVVHRPLAALDGATRNLLQTLAQFRQT

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=30.1204819277108, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790399, Length=275, Percent_Identity=26.1818181818182, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788887, Length=306, Percent_Identity=28.1045751633987, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI2367136, Length=144, Percent_Identity=35.4166666666667, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787601, Length=267, Percent_Identity=25.4681647940075, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788297, Length=242, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788748, Length=284, Percent_Identity=28.8732394366197, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=26.8965517241379, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=270, Percent_Identity=25.5555555555556, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=26.1224489795918, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788626, Length=196, Percent_Identity=30.1020408163265, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32249; Mature: 32249

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50931 HTH_LYSR ; PS01347 MRAY_1 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELNLDQLRTLVSIADLGTFSAAARALHLAQPTVSLHISELEARLGAQLVVRGGRSVVP
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEEHHHHHHHHHCCCEEEECCCEECC
TAAGAVLVERARRLLRDADDATDAVKRHVEGRLGRVRLGTSTGIVVHLLPRVLELMARAH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCC
PEIDVEVSILGSAETMERLGQGTLDVGLVATPQPARRELVMSHWRSDPMMAFIPTGWPAP
CCCEEEEEEECCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCC
KRITPPWLMDKPLIFNDSTTHMYRLTMEWFAKAGHTPRARIELNYTEAMKSLVVAGYGAA
CCCCCCCCCCCCEEECCCCCEEEEHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCHH
LLPLEQAGAAATPNGVQVLPLKPALTRHTAVVHRPLAALDGATRNLLQTLAQFRQT
CCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRELNLDQLRTLVSIADLGTFSAAARALHLAQPTVSLHISELEARLGAQLVVRGGRSVVP
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEEHHHHHHHHHCCCEEEECCCEECC
TAAGAVLVERARRLLRDADDATDAVKRHVEGRLGRVRLGTSTGIVVHLLPRVLELMARAH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCC
PEIDVEVSILGSAETMERLGQGTLDVGLVATPQPARRELVMSHWRSDPMMAFIPTGWPAP
CCCEEEEEEECCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCC
KRITPPWLMDKPLIFNDSTTHMYRLTMEWFAKAGHTPRARIELNYTEAMKSLVVAGYGAA
CCCCCCCCCCCCEEECCCCCEEEEHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCHH
LLPLEQAGAAATPNGVQVLPLKPALTRHTAVVHRPLAALDGATRNLLQTLAQFRQT
CCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]