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The map label for this gene is mnmC

Identifier: 91785773

GI number: 91785773

Start: 4847740

End: 4849710

Strand: Reverse

Name: mnmC

Synonym: Bxe_A4505

Alternate gene names: 91785773

Gene position: 4849710-4847740 (Counterclockwise)

Preceding gene: 91785775

Following gene: 91785772

Centisome position: 99.06

GC content: 67.28

Gene sequence:

>1971_bases
ATGACTGATCCGCTGATCCCCGCCGTCCTCGCTTTTCGCGCCAACGGCACGCCTTTTTCGCCGCTCTACGACGACATCTA
TCACAGCGCCGTGGGCGGTCTCGAACAGGCTCACTACGTTTTCCTGCGCGGCAATGCGCTGCCCGAGCGGTGGCAGGAGC
GTCGCGTTTTCACGGTAGTCGAAACCGGCTTCGGCATGGGCATCAACTTTCTGGTGACCTGGGCCGCGTGGCGCGCCGAT
TCCTCACGCTGCGAGCGGCTCCATTTCGTCTCGACTGAGAAGCATCCGTTTACCGTCGACGATTTACGCAAAGTGTATGC
GGCGACGATTTCCGATCCGGAGATCGCAGCCTTGGCGCAAGCGCTCGCGGATGCCTGGCCGATGCTCGTCCCCGGCACGC
ATCGGCTTGAATTCGAGGAAGGGCGCGTCGTACTCACGCTCGTTTTTGCCGACGCGCAGGACAGCGTGCCGGCCTTGAGG
CTGCGCGCCGATGCGTTCTACCTCGACGGCTTCGCGCCGGCCAGGAATCCCGAACTCTGGACTTCCGCGATCTTCAAGGC
GCTCGCGCGGCTGGCCGGCGAGGGTGCCACGTTCGCCACCTACAGCAGTGCCGGCGACATCAAGCGCGCGTTGACGCAGT
GTGGTTTTGAATACCGCAAGGTGGACGGCTTCGGCTGGAAACGCGCGATGCTGGTCGGCCGCTTCGCGCCGCGCTGGCGC
GTGCGCCGTCATGAGCCACCCGCGCCGCTCGTGGTCGACGAACGGCATGCCGTGGTGATCGGCACGGGCCTCGCCGGCTG
CGCGGTGATCGAGCGGCTGGCCGCGCGCGGCTGGCGCGTCACGTCGCTCGAACGGCATGCAGCCGTTGCTCAGGAAGCGT
CCGGCAATCCGGCCGGCGTGTTCCATCCCATGATTTCCCGTGACGACAGCGTCGCCTCGCGCGTCACGCGGACCGGTTTC
CTGTATTCGCTCAGGCATTGGGCCGCGCTCGAACGGCTCGGCTACGGACCATCGCGCGGCAGCAAGGGCCTGTTGCAGAT
TGCCGCGGATGACGAGGAAGCCTTGTCGATCAGCCAGGCGATCGCCGCGGCAAGCTATCCATCGGAGTACGTGACCTCCG
TCCCGGCAGACGAAGCTCAACGGCTGGCCGGCATGCCGCTCGCGCACGGCGGCTGGTTCTTTCCGTACGGCGGCTGGATC
GATCCTGCATCGCTCTGCGCCGCGCAGTGCGCCGCGGCAGGTCCGTTGCTCGAACGCCGCTTCGGGGTGGACGTCGCGCG
GATCGAGCGGGCGGGTGGTCAGTGGATCGTCTTCGACACCGCGGGTCAGGTTGTCGCGCGTGCGCCGGTGGTGATCGTTG
CGAGTGCTCACGATGCCGCGCGCATCGCCGGTTTGCAATACGCGCCGACTCGCAGCATTCGCGGCCAGCTCACGTTATTG
CCGGCCGGCACGGTGCGGCCGCCGCTCGAGTTGCCGGTGATCGGCGAAGGCTACGCCGTACCGCTTGCCAACGGCGCCAC
GCTGACCGGCGCCACCTATGAACTCGACGACCCCGACACCTCGCTTCGCACGGATGGCCATCTGGAAAACCTGGCGCGCG
TCGCGCAGATGTTGCCGGCGTTTGCGGGCATCGTCGATCGCGCCGATCCGGCGGCGTTGGCTGGACGTGTCGCGTTCCGT
TGCGTCACCAGCGACCGCATGCCGATGATCGGCCAGCTCGCCGACGAAACCGTGGCAGCGCGCGACGCCCAGCGTCTGCG
CGGCGCATGGCCGCTCGATCTGCCGCGCACAGACGGCCTGTACGGCGCGTTTGCCTACGGTTCGCGTGGCCTGGTGTGGG
CTGCGCTCGGCGCGGAACTGATCGCCTCGCAACTGGAAGGCGAACCGTGGCCGCTCGAACGGGATCTGGCTGAAGACATC
GATCCCGCGCGCTTCCTGTTGCGGGCATTGCGCCAGGGGACGGTGAGTTGA

Upstream 100 bases:

>100_bases
AAAACGCCGGGCCGCCTCAAGTTTTCTCACACCCTTGGAGGCCTTGGCGCCAGGCGACAGGTTCCGGGGGCATTTTTCCG
AGCCTTATTTTCCCTCGTGC

Downstream 100 bases:

>100_bases
CACGTGTTAACGCGGGCCGCCGGGCATCCTTGCCTGACCCGGTTATCCACCGACACTTGTGGATAACCGCGCCGTTTACC
CGCGCAACTCATGTGGAACC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MTDPLIPAVLAFRANGTPFSPLYDDIYHSAVGGLEQAHYVFLRGNALPERWQERRVFTVVETGFGMGINFLVTWAAWRAD
SSRCERLHFVSTEKHPFTVDDLRKVYAATISDPEIAALAQALADAWPMLVPGTHRLEFEEGRVVLTLVFADAQDSVPALR
LRADAFYLDGFAPARNPELWTSAIFKALARLAGEGATFATYSSAGDIKRALTQCGFEYRKVDGFGWKRAMLVGRFAPRWR
VRRHEPPAPLVVDERHAVVIGTGLAGCAVIERLAARGWRVTSLERHAAVAQEASGNPAGVFHPMISRDDSVASRVTRTGF
LYSLRHWAALERLGYGPSRGSKGLLQIAADDEEALSISQAIAAASYPSEYVTSVPADEAQRLAGMPLAHGGWFFPYGGWI
DPASLCAAQCAAAGPLLERRFGVDVARIERAGGQWIVFDTAGQVVARAPVVIVASAHDAARIAGLQYAPTRSIRGQLTLL
PAGTVRPPLELPVIGEGYAVPLANGATLTGATYELDDPDTSLRTDGHLENLARVAQMLPAFAGIVDRADPAALAGRVAFR
CVTSDRMPMIGQLADETVAARDAQRLRGAWPLDLPRTDGLYGAFAYGSRGLVWAALGAELIASQLEGEPWPLERDLAEDI
DPARFLLRALRQGTVS

Sequences:

>Translated_656_residues
MTDPLIPAVLAFRANGTPFSPLYDDIYHSAVGGLEQAHYVFLRGNALPERWQERRVFTVVETGFGMGINFLVTWAAWRAD
SSRCERLHFVSTEKHPFTVDDLRKVYAATISDPEIAALAQALADAWPMLVPGTHRLEFEEGRVVLTLVFADAQDSVPALR
LRADAFYLDGFAPARNPELWTSAIFKALARLAGEGATFATYSSAGDIKRALTQCGFEYRKVDGFGWKRAMLVGRFAPRWR
VRRHEPPAPLVVDERHAVVIGTGLAGCAVIERLAARGWRVTSLERHAAVAQEASGNPAGVFHPMISRDDSVASRVTRTGF
LYSLRHWAALERLGYGPSRGSKGLLQIAADDEEALSISQAIAAASYPSEYVTSVPADEAQRLAGMPLAHGGWFFPYGGWI
DPASLCAAQCAAAGPLLERRFGVDVARIERAGGQWIVFDTAGQVVARAPVVIVASAHDAARIAGLQYAPTRSIRGQLTLL
PAGTVRPPLELPVIGEGYAVPLANGATLTGATYELDDPDTSLRTDGHLENLARVAQMLPAFAGIVDRADPAALAGRVAFR
CVTSDRMPMIGQLADETVAARDAQRLRGAWPLDLPRTDGLYGAFAYGSRGLVWAALGAELIASQLEGEPWPLERDLAEDI
DPARFLLRALRQGTVS
>Mature_655_residues
TDPLIPAVLAFRANGTPFSPLYDDIYHSAVGGLEQAHYVFLRGNALPERWQERRVFTVVETGFGMGINFLVTWAAWRADS
SRCERLHFVSTEKHPFTVDDLRKVYAATISDPEIAALAQALADAWPMLVPGTHRLEFEEGRVVLTLVFADAQDSVPALRL
RADAFYLDGFAPARNPELWTSAIFKALARLAGEGATFATYSSAGDIKRALTQCGFEYRKVDGFGWKRAMLVGRFAPRWRV
RRHEPPAPLVVDERHAVVIGTGLAGCAVIERLAARGWRVTSLERHAAVAQEASGNPAGVFHPMISRDDSVASRVTRTGFL
YSLRHWAALERLGYGPSRGSKGLLQIAADDEEALSISQAIAAASYPSEYVTSVPADEAQRLAGMPLAHGGWFFPYGGWID
PASLCAAQCAAAGPLLERRFGVDVARIERAGGQWIVFDTAGQVVARAPVVIVASAHDAARIAGLQYAPTRSIRGQLTLLP
AGTVRPPLELPVIGEGYAVPLANGATLTGATYELDDPDTSLRTDGHLENLARVAQMLPAFAGIVDRADPAALAGRVAFRC
VTSDRMPMIGQLADETVAARDAQRLRGAWPLDLPRTDGLYGAFAYGSRGLVWAALGAELIASQLEGEPWPLERDLAEDID
PARFLLRALRQGTVS

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=673, Percent_Identity=33.5809806835067, Blast_Score=331, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_BURXL (Q13SL2)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_560979.1
- ProteinModelPortal:   Q13SL2
- SMR:   Q13SL2
- GeneID:   4006427
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A4505
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 70996; Mature: 70865

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPLIPAVLAFRANGTPFSPLYDDIYHSAVGGLEQAHYVFLRGNALPERWQERRVFTVV
CCCCHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCEEEEEE
ETGFGMGINFLVTWAAWRADSSRCERLHFVSTEKHPFTVDDLRKVYAATISDPEIAALAQ
ECCCCCCHHHHEEHHHHCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
ALADAWPMLVPGTHRLEFEEGRVVLTLVFADAQDSVPALRLRADAFYLDGFAPARNPELW
HHHHHCCEECCCCCEEEECCCCEEEEEEEECCCCCCCEEEEEECEEEECCCCCCCCCHHH
TSAIFKALARLAGEGATFATYSSAGDIKRALTQCGFEYRKVDGFGWKRAMLVGRFAPRWR
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCHH
VRRHEPPAPLVVDERHAVVIGTGLAGCAVIERLAARGWRVTSLERHAAVAQEASGNPAGV
CCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCEE
FHPMISRDDSVASRVTRTGFLYSLRHWAALERLGYGPSRGSKGLLQIAADDEEALSISQA
ECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHH
IAAASYPSEYVTSVPADEAQRLAGMPLAHGGWFFPYGGWIDPASLCAAQCAAAGPLLERR
HHHHCCCHHHHHCCCHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHH
FGVDVARIERAGGQWIVFDTAGQVVARAPVVIVASAHDAARIAGLQYAPTRSIRGQLTLL
HCCCHHHHHHCCCCEEEEECCCCHHHCCCEEEEECCCCHHHHCCCCCCCCCCCCCEEEEE
PAGTVRPPLELPVIGEGYAVPLANGATLTGATYELDDPDTSLRTDGHLENLARVAQMLPA
ECCCCCCCCCCCEECCCEEEEECCCCEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHH
FAGIVDRADPAALAGRVAFRCVTSDRMPMIGQLADETVAARDAQRLRGAWPLDLPRTDGL
HHHHHCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YGAFAYGSRGLVWAALGAELIASQLEGEPWPLERDLAEDIDPARFLLRALRQGTVS
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDPLIPAVLAFRANGTPFSPLYDDIYHSAVGGLEQAHYVFLRGNALPERWQERRVFTVV
CCCHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCEEEEEE
ETGFGMGINFLVTWAAWRADSSRCERLHFVSTEKHPFTVDDLRKVYAATISDPEIAALAQ
ECCCCCCHHHHEEHHHHCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
ALADAWPMLVPGTHRLEFEEGRVVLTLVFADAQDSVPALRLRADAFYLDGFAPARNPELW
HHHHHCCEECCCCCEEEECCCCEEEEEEEECCCCCCCEEEEEECEEEECCCCCCCCCHHH
TSAIFKALARLAGEGATFATYSSAGDIKRALTQCGFEYRKVDGFGWKRAMLVGRFAPRWR
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCHH
VRRHEPPAPLVVDERHAVVIGTGLAGCAVIERLAARGWRVTSLERHAAVAQEASGNPAGV
CCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCEE
FHPMISRDDSVASRVTRTGFLYSLRHWAALERLGYGPSRGSKGLLQIAADDEEALSISQA
ECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHH
IAAASYPSEYVTSVPADEAQRLAGMPLAHGGWFFPYGGWIDPASLCAAQCAAAGPLLERR
HHHHCCCHHHHHCCCHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHH
FGVDVARIERAGGQWIVFDTAGQVVARAPVVIVASAHDAARIAGLQYAPTRSIRGQLTLL
HCCCHHHHHHCCCCEEEEECCCCHHHCCCEEEEECCCCHHHHCCCCCCCCCCCCCEEEEE
PAGTVRPPLELPVIGEGYAVPLANGATLTGATYELDDPDTSLRTDGHLENLARVAQMLPA
ECCCCCCCCCCCEECCCEEEEECCCCEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHH
FAGIVDRADPAALAGRVAFRCVTSDRMPMIGQLADETVAARDAQRLRGAWPLDLPRTDGL
HHHHHCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YGAFAYGSRGLVWAALGAELIASQLEGEPWPLERDLAEDIDPARFLLRALRQGTVS
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA