BacMap

Definition	Burkholderia xenovorans LB400 chromosome 1, complete sequence.
Accession	NC_007951
Length	4,895,836

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The map label for this gene is fadB1 [H]

Identifier: 91785308

GI number: 91785308

Start: 4367537

End: 4368313

Strand: Reverse

Name: fadB1 [H]

Synonym: Bxe_A0471

Alternate gene names: 91785308

Gene position: 4368313-4367537 (Counterclockwise)

Preceding gene: 91785314

Following gene: 91785306

Centisome position: 89.23

GC content: 60.23

Gene sequence:

>777_bases
ATGGCTTACGAGAATATTCTGGTTGAGACGCGGGGGCGGGTCGGGTTGGTCACTTTGAATCGACCGAAGGCGCTGAACGC
GCTGAACGACGCATTGATGGACGAACTGGGCGCGGCGCTGCGCGAGTTCGATGCCGACGACGCGATCGGTGCGATCGTGG
TGACCGGCAGCGAGAAAGCGTTCGCGGCCGGTGCAGACGTCGGCATGATGTCCACTTATACCTATATGGATGTTTATAAG
GGGGACTACATCACCCGCAACTGGGAAACGGTCCGCTCCATCCGCAAGCCGATCATTGCGGCGGTGGCGGGCTTTGCGCT
TGGCGGCGGCTGCGAATTGGCGATGATGTGCGATATTATTTTTGCCGCCGACACGGCAAAGTTCGGTCAGCCGGAAATCA
AGCTCGGCATCATGCCGGGTGCCGGAGGCACGCAGCGGTTGCCGCGCGCCGTCTCCAAGGCGAAGGCAATGGACCTTTGC
CTGACCGCGCGTTTCATGGATGCCGCCGAAGCCGAGCGTGCCGGCCTGGTGTCGCGTGTTATTCCGGCTGCTTCTCTGGT
TGACGAGGCGGTAGCCGCGGCTGCCACGATCGCAGAATTCCCGTTGCCGGCCGTGATGATGGTGAAGGAATCGGTTAATC
GTGCTTATGAGACGACGCTCGCGGAAGGTGTGCATTTCGAGCGCCGCCTGTTCCATTCGCTCTTCGCTACCGAAGACCAG
AAGGAAGGCATGGCCGCGTTCGTGGAGAAGCGCAAACCGGTTTTCAAACATCGTTAA

Upstream 100 bases:

>100_bases
TCTAATAATTGACCGACCGGTTGGTTGGGGAATGTTAGCATTATTAGGATGGCCGCGCGAAGTGTTTTCGCGCGCGATGT
TTCTACCCTTAGGAGGTGGC

Downstream 100 bases:

>100_bases
TACGCTTGTCAAAATAACGCTTGCAAGGCGGAGTCCGGCTCGCTAGAATCTCGCTCTTTCGTGCTCCGGACAACGGGGCG
CGGGAGGCGGGAGAGCCAGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGSEKAFAAGADVGMMSTYTYMDVYK
GDYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLC
LTARFMDAAEAERAGLVSRVIPAASLVDEAVAAAATIAEFPLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKHR

Sequences:

>Translated_258_residues
MAYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGSEKAFAAGADVGMMSTYTYMDVYK
GDYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLC
LTARFMDAAEAERAGLVSRVIPAASLVDEAVAAAATIAEFPLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKHR
>Mature_257_residues
AYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGSEKAFAAGADVGMMSTYTYMDVYKG
DYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLCL
TARFMDAAEAERAGLVSRVIPAASLVDEAVAAAATIAEFPLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQK
EGMAAFVEKRKPVFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=54.4401544401544, Blast_Score=288, Evalue=3e-78,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=35.9375, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=32.3308270676692, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI68989263, Length=203, Percent_Identity=33.9901477832512, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=36.7231638418079, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.5573770491803, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=192, Percent_Identity=30.2083333333333, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=30.2083333333333, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=26.4227642276423, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=26.4227642276423, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=26.4227642276423, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=26.6375545851528, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=26.6375545851528, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.5136186770428, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.5136186770428, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=24.21875, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=24.21875, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757966, Length=205, Percent_Identity=26.3414634146341, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=205, Percent_Identity=26.3414634146341, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=36.1111111111111, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136756, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136753, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI157694520, Length=192, Percent_Identity=26.5625, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=43.4782608695652, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=35.7692307692308, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=34.3629343629344, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=29.6153846153846, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=30, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=30.5555555555556, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=54.6153846153846, Blast_Score=284, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.9094650205761, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.8181818181818, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=37.7049180327869, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=181, Percent_Identity=37.5690607734807, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=29.957805907173, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=181, Percent_Identity=37.5690607734807, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=181, Percent_Identity=37.5690607734807, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.953125, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=29.3233082706767, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=186, Percent_Identity=34.4086021505376, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=197, Percent_Identity=30.4568527918782, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=197, Percent_Identity=30.4568527918782, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=24.3243243243243, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=28.3505154639175, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=56.9767441860465, Blast_Score=293, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=56.9767441860465, Blast_Score=293, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=36.8421052631579, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=31.5384615384615, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=32.8638497652582, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=32.9411764705882, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654903, Length=246, Percent_Identity=26.0162601626016, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=25.6916996047431, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=32.183908045977, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=32.183908045977, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=32.183908045977, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571730, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27803; Mature: 27671

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGSEKA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADVGMMSTYTYMDVYKGDYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLCLTARFMDAAEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAASLVDEAVAAAATIAEFPLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGSEKA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADVGMMSTYTYMDVYKGDYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLCLTARFMDAAEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAASLVDEAVAAAATIAEFPLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]