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The map label for this gene is murD [H]

Identifier: 91785295

GI number: 91785295

Start: 4344231

End: 4345745

Strand: Reverse

Name: murD [H]

Synonym: Bxe_A0484

Alternate gene names: 91785295

Gene position: 4345745-4344231 (Counterclockwise)

Preceding gene: 91785296

Following gene: 91785294

Centisome position: 88.76

GC content: 67.19

Gene sequence:

>1515_bases
ATGTTCGGCGAGAAGTTTCGGGATCGGCAAAAGCCGATGGTGCTCGTGCTGGGACTGGGTGAATCCGGTCTCGCTATGGC
GCGCTGGTGCGCGCGGCACGGCTGTCGGCTGCGTGCGGCCGATACGCGCGAAGTGCCGCCGAACCTGTCCGCGCTCGAAG
CACACGGCGTCGACGCACAATTCGTCGGCGGCCCGTTTTCGCCGGTGCTGCTCGAAGGCGTGGAACTCGTCGCCATCAGT
CCGGGTTTGTCGCCGCTTGCCGCGGATCTGTTGCCGCTGATCGCGGCGGCGCGCGAGCAGGGCATCCCCGTGTGGGGCGA
ACTCGAATTTTTCTCGCAAGCGCTGAAGACGCTCGGCGAAAGCGGCTATGCGCCGAAAGTGATCGCGATCACCGGCACGA
ACGGCAAGACCACGACTACGAGCCTGACCGGCCTGCTGTGCGAGCGCGCCGGCAGGAAGGTCGCGGTGGCGGGCAATATC
AGCCCGGCGCTGCTCGACAAGCTCTCGGAAGCGATCGACAACACCGCGTTGCCGGACGTGTGGGTGCTGGAGCTATCGAG
CTTCCAGCTCGAAACCGCGCACACGTTTTCGCCTGACGCGGCGGTCGTGCTGAACATCACCCAGGATCACCTCGACTGGC
ACGGCGGACTGGACGCGTACGCTGCCGCGAAGGGCCGCATCTTTGGTACACAAACGGTGCGCGTGCTGAATCGCGACGAC
TCGCGCGTGATGAAGCTCGCGCCTTCCGAAGACAGCGAAGCCGGGATGGTCACGTTCGGCGTCACCGAGCCGAAGAACGA
GGGCGACTACGGCTTGCTGCGCGACAACGGCATGATCTGGCTGGTCGAGGCGCACGATCGCGACGCGAGCGACGAACCCG
CGCCGAAGCGCCGCCGCAAGAACGAAGTGGCGACGCCGCCGAACATCGCACTGAAGCGCCTGATGCCGGCAGACGCCTTG
CGCATTCGGGGTTTGCACAACGCCGCCAATGCGCTCGCCGCCTACGCGCTCGCACGCGCGATCGGCCTGCCGGGCGCGTC
GCTGCTGCACGGTCTGCGCGAATATCGCGGCGAGCCGCATCGCGTGGAATTGATCGCGTCGATCGACGGTATCGACTATG
TGGACGACAGCAAGGGCACCAATGTCGGCGCGACGGTCGCCGCGCTCGACGGACTCGCGCAGCGTGTGGTGCTGATCGCC
GGCGGTGACGGCAAGGGCCAGGATTTCGAACCGCTCGCGGCGCCCGTCATGCGCTGGTGCCGCGCTGTGATGCTGATCGG
CCGTGACGCGCCGCAGATTCGCGCCGCGCTGGAAGACACCGGTATCGCGATGACGGACCACGCCACGCTCGAAGAAGCAA
CGCGCGCCGCGGCCGCGCTGGCCCAGCCGGGCGACGCCGTACTGCTGTCGCCGGCCTGCGCGAGCTTCGACATGTTCAAG
GGTTACGCGCACCGCGCCGCGGTGTTCCGCGGCACGGTGGAAGAGATCGCAGCCGAACGGGGGACGATGATATGA

Upstream 100 bases:

>100_bases
AGGTGGTCGTGCGTTTCTGGATCATCACGTTGATGTTGTGTCTCTTTGGTTTGTCCACGCTCAAACTGCGTTAAGCAGCA
GGTATTAGGGGAAGCAGGCG

Downstream 100 bases:

>100_bases
GCTGGTCGGAACGTTTCGGTTCGCGTCAGGCTGCAGCCAGCGACGCAGGCGGTTTGGGCGGCGCGGGCCGGGTCGGCGGC
CGCACGGGCGGCGGCAGCGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRAADTREVPPNLSALEAHGVDAQFVGGPFSPVLLEGVELVAIS
PGLSPLAADLLPLIAAAREQGIPVWGELEFFSQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGRKVAVAGNI
SPALLDKLSEAIDNTALPDVWVLELSSFQLETAHTFSPDAAVVLNITQDHLDWHGGLDAYAAAKGRIFGTQTVRVLNRDD
SRVMKLAPSEDSEAGMVTFGVTEPKNEGDYGLLRDNGMIWLVEAHDRDASDEPAPKRRRKNEVATPPNIALKRLMPADAL
RIRGLHNAANALAAYALARAIGLPGASLLHGLREYRGEPHRVELIASIDGIDYVDDSKGTNVGATVAALDGLAQRVVLIA
GGDGKGQDFEPLAAPVMRWCRAVMLIGRDAPQIRAALEDTGIAMTDHATLEEATRAAAALAQPGDAVLLSPACASFDMFK
GYAHRAAVFRGTVEEIAAERGTMI

Sequences:

>Translated_504_residues
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRAADTREVPPNLSALEAHGVDAQFVGGPFSPVLLEGVELVAIS
PGLSPLAADLLPLIAAAREQGIPVWGELEFFSQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGRKVAVAGNI
SPALLDKLSEAIDNTALPDVWVLELSSFQLETAHTFSPDAAVVLNITQDHLDWHGGLDAYAAAKGRIFGTQTVRVLNRDD
SRVMKLAPSEDSEAGMVTFGVTEPKNEGDYGLLRDNGMIWLVEAHDRDASDEPAPKRRRKNEVATPPNIALKRLMPADAL
RIRGLHNAANALAAYALARAIGLPGASLLHGLREYRGEPHRVELIASIDGIDYVDDSKGTNVGATVAALDGLAQRVVLIA
GGDGKGQDFEPLAAPVMRWCRAVMLIGRDAPQIRAALEDTGIAMTDHATLEEATRAAAALAQPGDAVLLSPACASFDMFK
GYAHRAAVFRGTVEEIAAERGTMI
>Mature_504_residues
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRAADTREVPPNLSALEAHGVDAQFVGGPFSPVLLEGVELVAIS
PGLSPLAADLLPLIAAAREQGIPVWGELEFFSQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGRKVAVAGNI
SPALLDKLSEAIDNTALPDVWVLELSSFQLETAHTFSPDAAVVLNITQDHLDWHGGLDAYAAAKGRIFGTQTVRVLNRDD
SRVMKLAPSEDSEAGMVTFGVTEPKNEGDYGLLRDNGMIWLVEAHDRDASDEPAPKRRRKNEVATPPNIALKRLMPADAL
RIRGLHNAANALAAYALARAIGLPGASLLHGLREYRGEPHRVELIASIDGIDYVDDSKGTNVGATVAALDGLAQRVVLIA
GGDGKGQDFEPLAAPVMRWCRAVMLIGRDAPQIRAALEDTGIAMTDHATLEEATRAAAALAQPGDAVLLSPACASFDMFK
GYAHRAAVFRGTVEEIAAERGTMI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=497, Percent_Identity=38.0281690140845, Blast_Score=237, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 53593; Mature: 53593

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRAADTREVPPNLSALEAHGVDAQ
CCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCCE
FVGGPFSPVLLEGVELVAISPGLSPLAADLLPLIAAAREQGIPVWGELEFFSQALKTLGE
ECCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHCC
SGYAPKVIAITGTNGKTTTTSLTGLLCERAGRKVAVAGNISPALLDKLSEAIDNTALPDV
CCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCE
WVLELSSFQLETAHTFSPDAAVVLNITQDHLDWHGGLDAYAAAKGRIFGTQTVRVLNRDD
EEEEECCEEEEECCCCCCCCEEEEEEEHHHCCCCCCCCHHHHCCCCEECCEEEEEECCCC
SRVMKLAPSEDSEAGMVTFGVTEPKNEGDYGLLRDNGMIWLVEAHDRDASDEPAPKRRRK
CEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHC
NEVATPPNIALKRLMPADALRIRGLHNAANALAAYALARAIGLPGASLLHGLREYRGEPH
CCCCCCHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
RVELIASIDGIDYVDDSKGTNVGATVAALDGLAQRVVLIAGGDGKGQDFEPLAAPVMRWC
EEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHH
RAVMLIGRDAPQIRAALEDTGIAMTDHATLEEATRAAAALAQPGDAVLLSPACASFDMFK
HHHHHHCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
GYAHRAAVFRGTVEEIAAERGTMI
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRAADTREVPPNLSALEAHGVDAQ
CCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCCE
FVGGPFSPVLLEGVELVAISPGLSPLAADLLPLIAAAREQGIPVWGELEFFSQALKTLGE
ECCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHCC
SGYAPKVIAITGTNGKTTTTSLTGLLCERAGRKVAVAGNISPALLDKLSEAIDNTALPDV
CCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCE
WVLELSSFQLETAHTFSPDAAVVLNITQDHLDWHGGLDAYAAAKGRIFGTQTVRVLNRDD
EEEEECCEEEEECCCCCCCCEEEEEEEHHHCCCCCCCCHHHHCCCCEECCEEEEEECCCC
SRVMKLAPSEDSEAGMVTFGVTEPKNEGDYGLLRDNGMIWLVEAHDRDASDEPAPKRRRK
CEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHC
NEVATPPNIALKRLMPADALRIRGLHNAANALAAYALARAIGLPGASLLHGLREYRGEPH
CCCCCCHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
RVELIASIDGIDYVDDSKGTNVGATVAALDGLAQRVVLIAGGDGKGQDFEPLAAPVMRWC
EEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHH
RAVMLIGRDAPQIRAALEDTGIAMTDHATLEEATRAAAALAQPGDAVLLSPACASFDMFK
HHHHHHCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
GYAHRAAVFRGTVEEIAAERGTMI
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA