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The map label for this gene is proS [H]

Identifier: 91785266

GI number: 91785266

Start: 4312544

End: 4314280

Strand: Reverse

Name: proS [H]

Synonym: Bxe_A0513

Alternate gene names: 91785266

Gene position: 4314280-4312544 (Counterclockwise)

Preceding gene: 91785269

Following gene: 91785265

Centisome position: 88.12

GC content: 63.1

Gene sequence:

>1737_bases
ATGAAAGCTTCCCGTTTCTTTATCGGTACCCTCAAAGAAGCTCCCGCCGACGCAGAGATCGTCAGCCACAAGCTCATGGT
GCGCGCCGGCATGATCCGGCGCGTTGCCGGCGGTATCTATAACTACCTGCCGATCGGCTTGCGCTCGATCCGCAAGGTGG
AAGCCATCGTGCGCGAAGAAATGAACCGGGCAGGCGCGATCGAGTTGTTGATGCCGGCCGTGCAGCCGGCCGAGTTGTGG
CAGGAATCGGGCCGCTGGGAAAAGTACGGCCCCGAGCTGCTGCGCTTCAAGGACCGCAAGCAGTCCGATTTCGTGATCGG
ACCGACCCACGAAGAAGTGGTCACGGATATTGCGCGTAATCAGATCAAGAGCTACCGCCAGTTGCCGGTGAACTTCTATC
AGGTCCAGACAAAGTTCCGCGACGAGATCCGTCCGCGTTTCGGCGTGATGCGCGGGCGCGAATTCATCATGAAAGACGCG
TACTCGTTCGACAAGGACATGGACGGTCTGCGCGAGTCGTATCGCAAGATGTACGACGCGTACGTGCGCATCTTCACGCG
CCTCGGTCTGGACTTCCGCGCGGTGGCGGCGGACAACGGTTCGATCGGCGGCAGCGGCTCGCACGAATTCCACGTGATTG
CCGAGACCGGTGAGGACGCGATCGCCTATTGCCCGACCTCGGATTTCGCGGCCAACGTCGAGGCGGCTGAAGCGCTGCCG
CTCCACGCGGAACGCGCGGCGCCCGCCGAGGAAATGAAGAAGACCGCAACGCCGGGCAAGGCGAAGTGCGAAGCCGTGGC
CGAGTTGCTGAACATTCCGCTCGAACGCACCATCAAGTCGATCATTCTCGCAACCGAGAACGAAGGCGCCGAGCCGACCA
TCTGGCTGCTGATGCTGCGCGGCGACCACGATCTGAACGAGATCAAGGCGAGCAAGCTGCCGGGTCTGGCCGACTTCCGC
ATGGCGACCGAGGCTGAAATCATCGAAACCTTCGGCACGCCGCCGGGCTACCTCGGCCCGCTCAACACGAAGAAGCCGGT
CAAGGTCGTCGCGGATCGCACGGTCGCGAACATGAGCGACTTCGTGGTGGGCTCGAACGAGGTGGATTACCACACCACCG
GCGTGAACTGGGGCCGCGACCTGCCGGAACCGGTCGTCGCCGACATTCGCAACGTGAAGAAGGGCGATCCGTCGCCGGAC
GGCAAGGGTGTGATCGACATCTGCCGCGGTATCGAAGTGGGCCACGTGTTCCAGCTCGGCACCAAGTATTCCGAGGCAAT
GAACGCAACCTGCCTCGACGAAACCGGCAAGCCGCGGCCGATGGAAATGGGCTGCTACGGGATCGGCGTCACGCGTATCC
TCGGCGCCGCGATCGAACAGAATTTCGACGATAGAGGCATCATCTGGCCGGAATCGATCGCGCCGTTCGAAGTCGTGCTG
TGCCCGATGGGTTACGACCGCAGCGAAGCCGTACGCGAAGCCGCCGACAAGCTGTACGCAACGCTGCTCGAAGCGGGCAT
CGACGTGATCCTCGACGACCGCGGCGAACGCCCCGGCGTGATGTTCGCCGACTGGGAACTGATCGGCGTGCCGCATCGCC
TCGTGATCGGCGACCGTGGTCTCAAGGACGGCAAGCTCGAATATCAGGGCCGCCGCGACGCCGAGGCGACGCTGCTGCCG
GTTGAAGACGCCGCGCAAACGGTGATCGCGAAGGTTCGCGCAGCGTTGGCGCGCTAA

Upstream 100 bases:

>100_bases
CAGGTGAGCCGTGCCGGCTCAGAGCCGGATCTGTGTCGATTTCCCACCGTTTTCACCTTTCGGGCGGTCCCTCCGGAGCC
GCCGCTTTTGGATAATCTGA

Downstream 100 bases:

>100_bases
GCGGAGCGGACGGTGGAGTACACCTTCCTGTCCGCGACCATCCTGCTGATTCTGATCACCGACCCGCTCGGCAACATTCC
GCTCTTCATCAGCTGTTTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARNQIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDMDGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGEDAIAYCPTSDFAANVEAAEALP
LHAERAAPAEEMKKTATPGKAKCEAVAELLNIPLERTIKSIILATENEGAEPTIWLLMLRGDHDLNEIKASKLPGLADFR
MATEAEIIETFGTPPGYLGPLNTKKPVKVVADRTVANMSDFVVGSNEVDYHTTGVNWGRDLPEPVVADIRNVKKGDPSPD
GKGVIDICRGIEVGHVFQLGTKYSEAMNATCLDETGKPRPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPESIAPFEVVL
CPMGYDRSEAVREAADKLYATLLEAGIDVILDDRGERPGVMFADWELIGVPHRLVIGDRGLKDGKLEYQGRRDAEATLLP
VEDAAQTVIAKVRAALAR

Sequences:

>Translated_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARNQIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDMDGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGEDAIAYCPTSDFAANVEAAEALP
LHAERAAPAEEMKKTATPGKAKCEAVAELLNIPLERTIKSIILATENEGAEPTIWLLMLRGDHDLNEIKASKLPGLADFR
MATEAEIIETFGTPPGYLGPLNTKKPVKVVADRTVANMSDFVVGSNEVDYHTTGVNWGRDLPEPVVADIRNVKKGDPSPD
GKGVIDICRGIEVGHVFQLGTKYSEAMNATCLDETGKPRPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPESIAPFEVVL
CPMGYDRSEAVREAADKLYATLLEAGIDVILDDRGERPGVMFADWELIGVPHRLVIGDRGLKDGKLEYQGRRDAEATLLP
VEDAAQTVIAKVRAALAR
>Mature_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARNQIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDMDGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGEDAIAYCPTSDFAANVEAAEALP
LHAERAAPAEEMKKTATPGKAKCEAVAELLNIPLERTIKSIILATENEGAEPTIWLLMLRGDHDLNEIKASKLPGLADFR
MATEAEIIETFGTPPGYLGPLNTKKPVKVVADRTVANMSDFVVGSNEVDYHTTGVNWGRDLPEPVVADIRNVKKGDPSPD
GKGVIDICRGIEVGHVFQLGTKYSEAMNATCLDETGKPRPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPESIAPFEVVL
CPMGYDRSEAVREAADKLYATLLEAGIDVILDDRGERPGVMFADWELIGVPHRLVIGDRGLKDGKLEYQGRRDAEATLLP
VEDAAQTVIAKVRAALAR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=251, Percent_Identity=42.2310756972112, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=56.9686411149826, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=37.6984126984127, Blast_Score=178, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=41.4634146341463, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=551, Percent_Identity=29.4010889292196, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=37.9591836734694, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 64046; Mature: 64046

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDMDGLRESYRKMYDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGEDAIAYCPTSDFAANVEAAEALP
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHCCC
LHAERAAPAEEMKKTATPGKAKCEAVAELLNIPLERTIKSIILATENEGAEPTIWLLMLR
CCCHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKASKLPGLADFRMATEAEIIETFGTPPGYLGPLNTKKPVKVVADRTVANMSD
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGSNEVDYHTTGVNWGRDLPEPVVADIRNVKKGDPSPDGKGVIDICRGIEVGHVFQLG
HEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHC
TKYSEAMNATCLDETGKPRPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPESIAPFEVVL
CHHHHHCCCEEECCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSEAVREAADKLYATLLEAGIDVILDDRGERPGVMFADWELIGVPHRLVIGDRG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCEEEEECCCC
LKDGKLEYQGRRDAEATLLPVEDAAQTVIAKVRAALAR
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDMDGLRESYRKMYDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGEDAIAYCPTSDFAANVEAAEALP
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHCCC
LHAERAAPAEEMKKTATPGKAKCEAVAELLNIPLERTIKSIILATENEGAEPTIWLLMLR
CCCHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKASKLPGLADFRMATEAEIIETFGTPPGYLGPLNTKKPVKVVADRTVANMSD
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGSNEVDYHTTGVNWGRDLPEPVVADIRNVKKGDPSPDGKGVIDICRGIEVGHVFQLG
HEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHC
TKYSEAMNATCLDETGKPRPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPESIAPFEVVL
CHHHHHCCCEEECCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSEAVREAADKLYATLLEAGIDVILDDRGERPGVMFADWELIGVPHRLVIGDRG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCEEEEECCCC
LKDGKLEYQGRRDAEATLLPVEDAAQTVIAKVRAALAR
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA