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The map label for this gene is gdhA [H]

Identifier: 91785183

GI number: 91785183

Start: 4216829

End: 4218151

Strand: Reverse

Name: gdhA [H]

Synonym: Bxe_A0596

Alternate gene names: 91785183

Gene position: 4218151-4216829 (Counterclockwise)

Preceding gene: 91785184

Following gene: 91785182

Centisome position: 86.16

GC content: 63.34

Gene sequence:

>1323_bases
ATGTCATCCCAGCAACAGGCAGCACAACTCGCACAATCCGCAGCAACGTTGCACTCGGTTCCTTCCTACCTGAATAAAGA
CGACCTCGGCCCGTGGGGCAACTACCTGCGTCAGGTCGATCGCGTCGCGCCGTACCTCGGCTCCCTGTCCCGCTGGCTCG
AAACCCTGAAGCGCCCGAAGCGCATTCTGATCGTCGACGTGCCTATCGAACTCGATAACGGCACGGTCGCTCACTTCGAA
GGCTATCGCGTGCAGCACAACGTATCGCGCGGTCCGGGCAAGGGCGGTGTGCGTTATCACCAGGACGTGACGTTGTCGGA
AGTGATGGCGCTGTCGGCGTGGATGTCGGTGAAGAATGCTGCGGTGAACGTCCCGTACGGCGGCGCGAAGGGCGGCATCC
GTGTCGATCCGCGCACCTTGTCGCGTGGTGAGTTGGAGCGTGTGACGCGCCGCTATACCAGCGAAATCGGCATCATCATC
GGACCGAATACCGACATTCCCGCGCCGGACGTGAACACGAACGAGCAGATCATGGCGTGGATGATGGACACCTACTCGAT
GAACCAGGGCCAAACGGCCACGGGCGTCGTGACGGGCAAGCCGATCACGCTGGGCGGCTCGCTCGGCCGCCGCGAGGCAA
CCGGCCGCGGCGTGTTCGTGGTGGCGTCGGAAGCCGCACGCCGTATCGGCGTGGACATCGAAGGCGCGCGCATCGCGGTG
CAGGGTTTCGGCAACGTGGGCGGGATCGCGGCGCGTCTGTTCCAGGAAGCGGGTTCGAAGCTGGTCGCGGTGCAGGATCA
CACCGGCTCGCTGTACAAGTCCACGGGTATCGACGCGGTTGCATTGCTCGAGCACGTCGCAAAACACGGCGGCGTGGGCG
GCTTCCCGGAAGCGGATGCCGTGAGCAATGAAGAATTCTGGACGGTCGAATCGGACATCCTGATTCCGGCCGCGCTGGAA
AACCAGATCACCGAAAAGAACGCGGCGAAGATCAGGACGAAGATCGTCGTGGAAGGCGCCAACGGCCCGACCACTACGGC
GGCGGACGACATCCTGCACGATCGCGGCATTCTCGTGATTCCGGACGTGGTGGCCAACGCCGGCGGCGTGACCGTGTCGT
ACTTCGAATGGGTGCAGGACTTCTCGAGCTTCTTCTGGACGGAAGACGAGATCAACCAGCGTCTGGAGCGTGTGATGCGC
GAAGCGTTCGCCGCGGTGTGGCAGGTTTCGAGCGAGCAGAAGGTGTCCGTGCGGACCGCGGCGTTTATCGTCGCGTGTAA
GCGGATTCTGGAAGCGCGCGAACTGCGCGGCCTGTATCCCTGA

Upstream 100 bases:

>100_bases
ATGGTTATGCAAAAAAAACATAATCGGATAAGGAAACGGCATTGGATTTCAAAACGGCGTTTTTCGACAATGCTGTCATT
CGATCAACGCCGGAGCGTTC

Downstream 100 bases:

>100_bases
TTTTTCCGCTTCGAACGGCGGTTCTGTCATCTCGCAGAACCGCCGCCAATCCCTGTTTTGACGCGGCTCACGAGACCGCG
TGCGGGGCCCGGCGGGCGGC

Product: glutamate dehydrogenase (NADP)

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSSQQQAAQLAQSAATLHSVPSYLNKDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELDNGTVAHFE
GYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIII
GPNTDIPAPDVNTNEQIMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVASEAARRIGVDIEGARIAV
QGFGNVGGIAARLFQEAGSKLVAVQDHTGSLYKSTGIDAVALLEHVAKHGGVGGFPEADAVSNEEFWTVESDILIPAALE
NQITEKNAAKIRTKIVVEGANGPTTTAADDILHDRGILVIPDVVANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERVMR
EAFAAVWQVSSEQKVSVRTAAFIVACKRILEARELRGLYP

Sequences:

>Translated_440_residues
MSSQQQAAQLAQSAATLHSVPSYLNKDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELDNGTVAHFE
GYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIII
GPNTDIPAPDVNTNEQIMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVASEAARRIGVDIEGARIAV
QGFGNVGGIAARLFQEAGSKLVAVQDHTGSLYKSTGIDAVALLEHVAKHGGVGGFPEADAVSNEEFWTVESDILIPAALE
NQITEKNAAKIRTKIVVEGANGPTTTAADDILHDRGILVIPDVVANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERVMR
EAFAAVWQVSSEQKVSVRTAAFIVACKRILEARELRGLYP
>Mature_439_residues
SSQQQAAQLAQSAATLHSVPSYLNKDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELDNGTVAHFEG
YRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIG
PNTDIPAPDVNTNEQIMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVASEAARRIGVDIEGARIAVQ
GFGNVGGIAARLFQEAGSKLVAVQDHTGSLYKSTGIDAVALLEHVAKHGGVGGFPEADAVSNEEFWTVESDILIPAALEN
QITEKNAAKIRTKIVVEGANGPTTTAADDILHDRGILVIPDVVANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERVMRE
AFAAVWQVSSEQKVSVRTAAFIVACKRILEARELRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=44.8979591836735, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI4885281, Length=343, Percent_Identity=43.4402332361516, Blast_Score=282, Evalue=4e-76,
Organism=Escherichia coli, GI1788059, Length=360, Percent_Identity=32.5, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=377, Percent_Identity=40.053050397878, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6319256, Length=397, Percent_Identity=30.4785894206549, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=380, Percent_Identity=29.4736842105263, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=43.0939226519337, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45549226, Length=353, Percent_Identity=43.9093484419263, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24649283, Length=331, Percent_Identity=43.8066465256798, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649460, Length=319, Percent_Identity=42.3197492163009, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47698; Mature: 47566

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQQQAAQLAQSAATLHSVPSYLNKDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RILIVDVPIELDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNA
EEEEEEEEEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
AVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQIMAW
EEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHH
MMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVASEAARRIGVDIEGARIAV
HHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEEECHHHHHHCCCCCCCEEEE
QGFGNVGGIAARLFQEAGSKLVAVQDHTGSLYKSTGIDAVALLEHVAKHGGVGGFPEADA
EECCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
VSNEEFWTVESDILIPAALENQITEKNAAKIRTKIVVEGANGPTTTAADDILHDRGILVI
CCCCCEEEECCCEEECHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHCCCCEEE
PDVVANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERVMREAFAAVWQVSSEQKVSVRTA
CHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AFIVACKRILEARELRGLYP
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSQQQAAQLAQSAATLHSVPSYLNKDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RILIVDVPIELDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNA
EEEEEEEEEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
AVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQIMAW
EEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHH
MMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVASEAARRIGVDIEGARIAV
HHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEEECHHHHHHCCCCCCCEEEE
QGFGNVGGIAARLFQEAGSKLVAVQDHTGSLYKSTGIDAVALLEHVAKHGGVGGFPEADA
EECCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
VSNEEFWTVESDILIPAALENQITEKNAAKIRTKIVVEGANGPTTTAADDILHDRGILVI
CCCCCEEEECCCEEECHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHCCCCEEE
PDVVANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERVMREAFAAVWQVSSEQKVSVRTA
CHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AFIVACKRILEARELRGLYP
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]