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The map label for this gene is mepA [H]

Identifier: 91785072

GI number: 91785072

Start: 4101545

End: 4102777

Strand: Reverse

Name: mepA [H]

Synonym: Bxe_A0708

Alternate gene names: 91785072

Gene position: 4102777-4101545 (Counterclockwise)

Preceding gene: 91785074

Following gene: 91785071

Centisome position: 83.8

GC content: 66.83

Gene sequence:

>1233_bases
ATGCGCGTCGAACGGGTTCCATTCCGCTTAATCAGTGCCGCGACGGCTGCCGTATTGCTGGCAGCGTGCGGACCAAAACA
ATCTGCGCCGCCGCCCCAAACGCCCGAAGTCGGCGTTGTCACAGTTCAGCCGACGGCCGTCCCCGTCGTCACCGAATTGC
CCGGCCGCACCAGTGCCTTCCTCGTCGCGCAAGTGCGCGCGCGGGTCGACGGCATCGTGCTGCGCCGCGAGTTCACGGAA
GGCAGCCAGGTCAAGGCGGGTCAGCGTCTGTACAAGATCGATCCGGCGCCGTACATCGCCACGTTGAACAACGCCAAGGC
GACGCTGGCGAAGGCGCAGGCCAATCTCGTGTCCACCACGGCGCAGGCCAATCGCTACAAGGTGCTGGTTGCCGCCAACG
CGGTCAGCAAGCAGGACTACGACAACGCGGTCGCGGCGGAAGGCCAGGCGGCGGCTGACGTCGCAGCGGGCAAGGCGGCG
GTCGACACGGCGCAGATCAATCTGGGCTACACCGACGTCGTGTCGCCGGTGAGCGGCCAGGTCGGCATCTCGCAGGTCAC
GCCGGGCGCCTACGTGCAAGCCAGCGCCGCCACGCTGATGGCCACCGTGCAGACACTCGATCCGGTCTATGTGGACCTGA
CGCAATCGAGCCTCGACGGCCTGAAGCTGCGCCGCGACATGCAGGAAGGGCGCCTGAAAACGACGGGTCCGGACGCCGCC
AGAGTGTCGCTGATTCTCGAAGACGGCCGCACCTATTCGGAAAAGGGCAAGCTGCAGTTCACCGACGTGACGGTCGACCA
GGGCACCGGTTCGGTCACGGTGCGCGCGATCTTCAAGAACGCCGACCGGGTGCTGCTGCCTGGCATGTTCGTGCGCGCGC
GCATCGACGAAGGCGTGAACGACAACGCGCTCGTGGTGCCGCAAGTCGGCGTGACGCACGACCAGAAGGGGCAGCCCACC
GCGCTGGTCGTCGGTAACGACAACAAGGTCGCACTGCGTCAACTGGTTACGTCCGGCACGTATGGTTCGAACTGGGTGGT
CGAAAGCGGCCTGAATCCGGGCGACCGCGTGATCGTGCAGGGCACCGACAAGGCGAAACCCGGCATGCAGGTCAAGGCCG
TTGCCGCGCAACTTCCCGCCACACCCGCTTCCGACGCCTCCGCTCAGGGCGCGCCGGCCGCCAGCGGTGCTGCGCAGATG
GCTCAACCTGCCTCCGCTGCATCGGGCGCGTAA

Upstream 100 bases:

>100_bases
CATCCGGTCAGGCGTCGCGAGACGCATGCGTGCGCTGCTGTCCCCGGCGTAGGGATGCTCGCACTTTTTCATTCTCTCAG
TCTTAGACAGAGGTCGCTCC

Downstream 100 bases:

>100_bases
TAACAGGGAGCCCGCCTCATGGCAAAGTTCTTCATTGATCGCCCGATTTTTGCATGGGTGATCGCCATTATTCTGATGCT
CGCGGGTATCGCGTCGGTCT

Product: membrane fusion protein, RND efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MRVERVPFRLISAATAAVLLAACGPKQSAPPPQTPEVGVVTVQPTAVPVVTELPGRTSAFLVAQVRARVDGIVLRREFTE
GSQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLVSTTAQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAA
VDTAQINLGYTDVVSPVSGQVGISQVTPGAYVQASAATLMATVQTLDPVYVDLTQSSLDGLKLRRDMQEGRLKTTGPDAA
RVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFKNADRVLLPGMFVRARIDEGVNDNALVVPQVGVTHDQKGQPT
ALVVGNDNKVALRQLVTSGTYGSNWVVESGLNPGDRVIVQGTDKAKPGMQVKAVAAQLPATPASDASAQGAPAASGAAQM
AQPASAASGA

Sequences:

>Translated_410_residues
MRVERVPFRLISAATAAVLLAACGPKQSAPPPQTPEVGVVTVQPTAVPVVTELPGRTSAFLVAQVRARVDGIVLRREFTE
GSQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLVSTTAQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAA
VDTAQINLGYTDVVSPVSGQVGISQVTPGAYVQASAATLMATVQTLDPVYVDLTQSSLDGLKLRRDMQEGRLKTTGPDAA
RVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFKNADRVLLPGMFVRARIDEGVNDNALVVPQVGVTHDQKGQPT
ALVVGNDNKVALRQLVTSGTYGSNWVVESGLNPGDRVIVQGTDKAKPGMQVKAVAAQLPATPASDASAQGAPAASGAAQM
AQPASAASGA
>Mature_410_residues
MRVERVPFRLISAATAAVLLAACGPKQSAPPPQTPEVGVVTVQPTAVPVVTELPGRTSAFLVAQVRARVDGIVLRREFTE
GSQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLVSTTAQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAA
VDTAQINLGYTDVVSPVSGQVGISQVTPGAYVQASAATLMATVQTLDPVYVDLTQSSLDGLKLRRDMQEGRLKTTGPDAA
RVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFKNADRVLLPGMFVRARIDEGVNDNALVVPQVGVTHDQKGQPT
ALVVGNDNKVALRQLVTSGTYGSNWVVESGLNPGDRVIVQGTDKAKPGMQVKAVAAQLPATPASDASAQGAPAASGAAQM
AQPASAASGA

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=51.9021739130435, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789665, Length=381, Percent_Identity=48.2939632545932, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1789929, Length=368, Percent_Identity=47.0108695652174, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87082036, Length=349, Percent_Identity=32.0916905444126, Blast_Score=147, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42373; Mature: 42373

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPFRLISAATAAVLLAACGPKQSAPPPQTPEVGVVTVQPTAVPVVTELPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGSQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLVSTT
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHC
AQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAAVDTAQINLGYTDVVSPVSGQ
CCCCEEEEEEEECCCCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHCCCCCCC
VGISQVTPGAYVQASAATLMATVQTLDPVYVDLTQSSLDGLKLRRDMQEGRLKTTGPDAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCE
RVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFKNADRVLLPGMFVRARIDEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCEEEECCEEEEEEECCCCC
DNALVVPQVGVTHDQKGQPTALVVGNDNKVALRQLVTSGTYGSNWVVESGLNPGDRVIVQ
CCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEE
GTDKAKPGMQVKAVAAQLPATPASDASAQGAPAASGAAQMAQPASAASGA
CCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MRVERVPFRLISAATAAVLLAACGPKQSAPPPQTPEVGVVTVQPTAVPVVTELPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGSQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLVSTT
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHC
AQANRYKVLVAANAVSKQDYDNAVAAEGQAAADVAAGKAAVDTAQINLGYTDVVSPVSGQ
CCCCEEEEEEEECCCCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHCCCCCCC
VGISQVTPGAYVQASAATLMATVQTLDPVYVDLTQSSLDGLKLRRDMQEGRLKTTGPDAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCE
RVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFKNADRVLLPGMFVRARIDEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCEEEECCEEEEEEECCCCC
DNALVVPQVGVTHDQKGQPTALVVGNDNKVALRQLVTSGTYGSNWVVESGLNPGDRVIVQ
CCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEE
GTDKAKPGMQVKAVAAQLPATPASDASAQGAPAASGAAQMAQPASAASGA
CCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]