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The map label for this gene is bcr [H]

Identifier: 91784893

GI number: 91784893

Start: 3893952

End: 3895202

Strand: Reverse

Name: bcr [H]

Synonym: Bxe_A0898

Alternate gene names: 91784893

Gene position: 3895202-3893952 (Counterclockwise)

Preceding gene: 91784902

Following gene: 91784892

Centisome position: 79.56

GC content: 64.19

Gene sequence:

>1251_bases
ATGGCGGCGCGGCGCTTCATCTGGATTCCCATGTCTCACGTCACCAGAAGCCGGCCCGATGGCCGGCTGATTCTGTTGCT
CGGTGCGCTCGCCGCATGCGGGCCGATTTCGATCGATATGTATCTGCCGAGCCTGCCGACCATCGCGCAGGCGTTCGCTA
TCAGCACCGGCGCTGCGCAGACCACGCTCACCAGTTTCATGTTCGGCTTTTCGATCGGTATGCTGCTGTATGGTCCGCTG
TCGGATACCTATGGCCGCCGGCCGGTGCTGCTCGGCGGGATCGTCATGTATGCGCTGGCCAGCGTGGCCTGTGCGTTGTC
GTTCTCGATCGGCTCGCTGGTGATATTCCGGTTCGTGCAGGCGCTGGGCGCGGGCGCGGCTTCGGTGCTGGCGCGCGCCA
TTGCACGCGACGCCCACGGACCGGCCGACGCCGCCCGCGTGCTGTCCATGCTCGCCATCGTCACATCGATCGGGCCTTTG
CTTGCGCCGCTGATCGGGGGGCAGTTGCTGCTGCTCGGCGGCTGGCGCGTCGTCTTCATCGTGCTGACGCTGTTCGGTAC
GGTGTGTGCGGTCACCGCGTTTCTCAAGGTGCCGGAAACGTGGCCGCGCGAGAAGCGCGCGCAGTCGGCGCTGCTGAAAT
CGTTCGGCGCATACGGCAAGTTGTTGCGCGATCCGGTTGCGTGGGGGCACATGCTGTGCGGCGGCATGGCGTTCGCGTCG
ATGTTCGCGTACATCACGGCAACGCCGTTCGTCTACATCGAGTATTTCCATGTGTCGGCGCAGCATTACGGCTTCCTGTT
CGCGTTGAATATCGTCGGCATCATGTTCGGCAACTTCATGAATACGCGGCTTGTCGGCCGGCTTGGCCCGCTGCCGCTCA
TTTCTTTTGCCGCAACCGTGAGTTGCATTGCCTCGCTGTTCGTCTGTCTCGTATCGCTGACCGGTTGGGGCGGTCTGTGG
TCGATCGTGTTCGGCCTGTTTTTCGTGGTCGGTGTGGTGGGTCTTCTGTCCGCCAACTGCACGACCGATCTGATGCATCG
CTATCCGGTGAATGCCGGTGCTGCGGCTGCGGTGTTCGGCGCCGTGCAGCTGGCGCTCGGCGCGCTGTCGAGCCTCGCGG
TCGGACTCTGGCAGGATGGCTCGCCCAAAGGTATGGGCGTGGTGGTGGGCGCGGCGGGCCTGCTCTGTTATCTCGGCCGG
ATTCTGGTCGTCAGGTGGCACGGAACCAGGGCGCCCGTTGCTGTCGTTTAA

Upstream 100 bases:

>100_bases
GCCAACCTGTACGAAGTCGAAGTCCGCGAGCGCATTAACCGTCGAGTACAATTGAAACTGTCAGGTTGCTGCTTTCCCCG
TTTCACCTGTTCCGCTGCCG

Downstream 100 bases:

>100_bases
AGCCTGGTGAGGCGCAAGCCGCGCGGCGTCTACGGAAAAAACACCAGGTGTTGCGCGCACGCGACCGTGCGTCTCAATTT
GCATTGCTATGATGGAGCCA

Product: major facilitator superfamily multidrug efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAARRFIWIPMSHVTRSRPDGRLILLLGALAACGPISIDMYLPSLPTIAQAFAISTGAAQTTLTSFMFGFSIGMLLYGPL
SDTYGRRPVLLGGIVMYALASVACALSFSIGSLVIFRFVQALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPL
LAPLIGGQLLLLGGWRVVFIVLTLFGTVCAVTAFLKVPETWPREKRAQSALLKSFGAYGKLLRDPVAWGHMLCGGMAFAS
MFAYITATPFVYIEYFHVSAQHYGFLFALNIVGIMFGNFMNTRLVGRLGPLPLISFAATVSCIASLFVCLVSLTGWGGLW
SIVFGLFFVVGVVGLLSANCTTDLMHRYPVNAGAAAAVFGAVQLALGALSSLAVGLWQDGSPKGMGVVVGAAGLLCYLGR
ILVVRWHGTRAPVAVV

Sequences:

>Translated_416_residues
MAARRFIWIPMSHVTRSRPDGRLILLLGALAACGPISIDMYLPSLPTIAQAFAISTGAAQTTLTSFMFGFSIGMLLYGPL
SDTYGRRPVLLGGIVMYALASVACALSFSIGSLVIFRFVQALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPL
LAPLIGGQLLLLGGWRVVFIVLTLFGTVCAVTAFLKVPETWPREKRAQSALLKSFGAYGKLLRDPVAWGHMLCGGMAFAS
MFAYITATPFVYIEYFHVSAQHYGFLFALNIVGIMFGNFMNTRLVGRLGPLPLISFAATVSCIASLFVCLVSLTGWGGLW
SIVFGLFFVVGVVGLLSANCTTDLMHRYPVNAGAAAAVFGAVQLALGALSSLAVGLWQDGSPKGMGVVVGAAGLLCYLGR
ILVVRWHGTRAPVAVV
>Mature_415_residues
AARRFIWIPMSHVTRSRPDGRLILLLGALAACGPISIDMYLPSLPTIAQAFAISTGAAQTTLTSFMFGFSIGMLLYGPLS
DTYGRRPVLLGGIVMYALASVACALSFSIGSLVIFRFVQALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPLL
APLIGGQLLLLGGWRVVFIVLTLFGTVCAVTAFLKVPETWPREKRAQSALLKSFGAYGKLLRDPVAWGHMLCGGMAFASM
FAYITATPFVYIEYFHVSAQHYGFLFALNIVGIMFGNFMNTRLVGRLGPLPLISFAATVSCIASLFVCLVSLTGWGGLWS
IVFGLFFVVGVVGLLSANCTTDLMHRYPVNAGAAAAVFGAVQLALGALSSLAVGLWQDGSPKGMGVVVGAAGLLCYLGRI
LVVRWHGTRAPVAVV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=355, Percent_Identity=36.9014084507042, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI48994889, Length=364, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082312, Length=354, Percent_Identity=27.683615819209, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790146, Length=236, Percent_Identity=27.5423728813559, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787065, Length=259, Percent_Identity=27.7992277992278, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790794, Length=229, Percent_Identity=23.1441048034935, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=161, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319480, Length=341, Percent_Identity=21.1143695014663, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=24.4047619047619, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43788; Mature: 43657

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARRFIWIPMSHVTRSRPDGRLILLLGALAACGPISIDMYLPSLPTIAQAFAISTGAAQ
CCCCEEEEEEHHHHHCCCCCCCEEEHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCHH
TTLTSFMFGFSIGMLLYGPLSDTYGRRPVLLGGIVMYALASVACALSFSIGSLVIFRFVQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLLLLGGWRVVFI
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHH
VLTLFGTVCAVTAFLKVPETWPREKRAQSALLKSFGAYGKLLRDPVAWGHMLCGGMAFAS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFAYITATPFVYIEYFHVSAQHYGFLFALNIVGIMFGNFMNTRLVGRLGPLPLISFAATV
HHHHHHHCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SCIASLFVCLVSLTGWGGLWSIVFGLFFVVGVVGLLSANCTTDLMHRYPVNAGAAAAVFG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHH
AVQLALGALSSLAVGLWQDGSPKGMGVVVGAAGLLCYLGRILVVRWHGTRAPVAVV
HHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHEEEECCCCCCEEEC
>Mature Secondary Structure 
AARRFIWIPMSHVTRSRPDGRLILLLGALAACGPISIDMYLPSLPTIAQAFAISTGAAQ
CCCEEEEEEHHHHHCCCCCCCEEEHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCHH
TTLTSFMFGFSIGMLLYGPLSDTYGRRPVLLGGIVMYALASVACALSFSIGSLVIFRFVQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGAGAASVLARAIARDAHGPADAARVLSMLAIVTSIGPLLAPLIGGQLLLLGGWRVVFI
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHH
VLTLFGTVCAVTAFLKVPETWPREKRAQSALLKSFGAYGKLLRDPVAWGHMLCGGMAFAS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFAYITATPFVYIEYFHVSAQHYGFLFALNIVGIMFGNFMNTRLVGRLGPLPLISFAATV
HHHHHHHCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SCIASLFVCLVSLTGWGGLWSIVFGLFFVVGVVGLLSANCTTDLMHRYPVNAGAAAAVFG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHH
AVQLALGALSSLAVGLWQDGSPKGMGVVVGAAGLLCYLGRILVVRWHGTRAPVAVV
HHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHEEEECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]