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The map label for this gene is nodE [H]

Identifier: 91784855

GI number: 91784855

Start: 3854502

End: 3855683

Strand: Reverse

Name: nodE [H]

Synonym: Bxe_A0937

Alternate gene names: 91784855

Gene position: 3855683-3854502 (Counterclockwise)

Preceding gene: 91784856

Following gene: 91784854

Centisome position: 78.75

GC content: 67.68

Gene sequence:

>1182_bases
ATGAAGGCGCCATCAGTTTATTTGCACGCGCTCGGCATGATCAACGCGCTCGGCGGCGACCTCGACAGCATTGTCCCGGC
GCTTGCCGCCGGCCGTTCGCCGGGTATGGCGAACGCGCATACGGGGATCGGCGAGGCGTTCGTCGGCAGCGTGCTCGCGC
CGCTGGAGCTGGCGCCGCGCGCCGAACTCGCCCGCTACGACTGCCGCAATAACCGCTTGCTGCTCGCCGCGCTGGCGCAG
ATCGCGCCCGCTGTTGAAGCGGCGCGCGAACGTTACGGCGCGCATCGCGTCGGCGTGGTGCTCGGCACCAGCACGTCGGG
CATCGAGGCCGCTGAAGCCGCGTTCGTCTATCAGGCGCAGGCCGGCGATCTTCCCGCGAACTTCAACTACCGGCAGATGG
AAATCGGCACCGCCGCGCCGTTCGCCGCCGCCGCGCTCGGCCTGCACGGTCCGGCGTTCACCATTTCGACCGCGTGCACA
TCGAGCGCGAAAGCCTTTGCGTCGGCACGCCGGCTGTTGCAGTTGCAACTATGCGATGCGGTCGTGGTGGGCGGTGTCGA
TTCGCTGTGCGAGCTCACGGTGCAGGGTTTCGCATCGCTCGAATCGACCAGCAACGTGCGCAGCAATCCGATGAGCCGCA
ATCGTTGCGGGATCAATGTCGGCGAAGGCGCGGCCGTGTTCCTCATGAGCCGCGACGAGGCGATGGTGCGCGTCGCGGGC
GTGGGCGAATCGAGCGACGCGCATCACATTTCCTCGCCGGACCCGCAGGGCGTGGGCGGCGAACTCGCGCTGCGCGCGGC
GCTCGCGGATGCGGGTGTCGAATCGGCGGCGATCGGCTACGTGAATCTGCACGCCACCGCCACGCGCAAGAACGACGAGA
TGGAAGCCCACCTGATGTCGCGCGTCTTTCCCGGCGGCGTGCCGACCAGCGGCACCAAGCCGTTGACCGGCCACCAGCTC
GGCGCGGCGGGCGCGACCGAACTCGGCTTCGCCTGGCTGACGCTGGCGCGTGAGAACGTGCCGTTGCCGCGTCATCTGTG
GGACGGTGAAGCCGATCCGGCATTGCCGCCGCTGGATCTCGTGGAAAGCGAGCGGTTTTTGCCGCAAGGCGGCGCGCAGT
ACGCAATGAGCAATTCATTTGCTTTCGGCGGCAGCAACGTCAGCCTGATTCTTGCCCGATGA

Upstream 100 bases:

>100_bases
TGCCCGCGCTGAAAAGTTTTGGCGCGACGCTCGCGCTCGGCATCGCGGTCTCGGTGCTGCTCGCGCCGATCGGCATGCCA
TCGGAATCAAGGAGGGCCGC

Downstream 100 bases:

>100_bases
GCCACGCCATGAACCCGACGCGCACCGTTCAGGAACTGGTCCGGCAGCCGATCGAGGCGATCATCCCGCATCGCGGCACC
ATGCTGCTGATCGACGCCGT

Product: 3-oxoacyl-(acyl carrier protein) synthase I

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKAPSVYLHALGMINALGGDLDSIVPALAAGRSPGMANAHTGIGEAFVGSVLAPLELAPRAELARYDCRNNRLLLAALAQ
IAPAVEAARERYGAHRVGVVLGTSTSGIEAAEAAFVYQAQAGDLPANFNYRQMEIGTAAPFAAAALGLHGPAFTISTACT
SSAKAFASARRLLQLQLCDAVVVGGVDSLCELTVQGFASLESTSNVRSNPMSRNRCGINVGEGAAVFLMSRDEAMVRVAG
VGESSDAHHISSPDPQGVGGELALRAALADAGVESAAIGYVNLHATATRKNDEMEAHLMSRVFPGGVPTSGTKPLTGHQL
GAAGATELGFAWLTLARENVPLPRHLWDGEADPALPPLDLVESERFLPQGGAQYAMSNSFAFGGSNVSLILAR

Sequences:

>Translated_393_residues
MKAPSVYLHALGMINALGGDLDSIVPALAAGRSPGMANAHTGIGEAFVGSVLAPLELAPRAELARYDCRNNRLLLAALAQ
IAPAVEAARERYGAHRVGVVLGTSTSGIEAAEAAFVYQAQAGDLPANFNYRQMEIGTAAPFAAAALGLHGPAFTISTACT
SSAKAFASARRLLQLQLCDAVVVGGVDSLCELTVQGFASLESTSNVRSNPMSRNRCGINVGEGAAVFLMSRDEAMVRVAG
VGESSDAHHISSPDPQGVGGELALRAALADAGVESAAIGYVNLHATATRKNDEMEAHLMSRVFPGGVPTSGTKPLTGHQL
GAAGATELGFAWLTLARENVPLPRHLWDGEADPALPPLDLVESERFLPQGGAQYAMSNSFAFGGSNVSLILAR
>Mature_393_residues
MKAPSVYLHALGMINALGGDLDSIVPALAAGRSPGMANAHTGIGEAFVGSVLAPLELAPRAELARYDCRNNRLLLAALAQ
IAPAVEAARERYGAHRVGVVLGTSTSGIEAAEAAFVYQAQAGDLPANFNYRQMEIGTAAPFAAAALGLHGPAFTISTACT
SSAKAFASARRLLQLQLCDAVVVGGVDSLCELTVQGFASLESTSNVRSNPMSRNRCGINVGEGAAVFLMSRDEAMVRVAG
VGESSDAHHISSPDPQGVGGELALRAALADAGVESAAIGYVNLHATATRKNDEMEAHLMSRVFPGGVPTSGTKPLTGHQL
GAAGATELGFAWLTLARENVPLPRHLWDGEADPALPPLDLVESERFLPQGGAQYAMSNSFAFGGSNVSLILAR

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=411, Percent_Identity=30.1703163017032, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI223972627, Length=157, Percent_Identity=36.3057324840764, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1787337, Length=270, Percent_Identity=35.5555555555556, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788663, Length=275, Percent_Identity=31.2727272727273, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983541, Length=410, Percent_Identity=28.0487804878049, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71983535, Length=261, Percent_Identity=29.8850574712644, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI212642053, Length=264, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320904, Length=277, Percent_Identity=33.9350180505415, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644341, Length=265, Percent_Identity=33.2075471698113, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 40564; Mature: 40564

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPSVYLHALGMINALGGDLDSIVPALAAGRSPGMANAHTGIGEAFVGSVLAPLELAPR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
AELARYDCRNNRLLLAALAQIAPAVEAARERYGAHRVGVVLGTSTSGIEAAEAAFVYQAQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHEEEEEEC
AGDLPANFNYRQMEIGTAAPFAAAALGLHGPAFTISTACTSSAKAFASARRLLQLQLCDA
CCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
VVVGGVDSLCELTVQGFASLESTSNVRSNPMSRNRCGINVGEGAAVFLMSRDEAMVRVAG
HHHCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEECCCCEEEEEECCCEEEEEEE
VGESSDAHHISSPDPQGVGGELALRAALADAGVESAAIGYVNLHATATRKNDEMEAHLMS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEEEECCCCCHHHHHHHH
RVFPGGVPTSGTKPLTGHQLGAAGATELGFAWLTLARENVPLPRHLWDGEADPALPPLDL
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHH
VESERFLPQGGAQYAMSNSFAFGGSNVSLILAR
HCCCCCCCCCCCCHHCCCCEEECCCCEEEEEEC
>Mature Secondary Structure
MKAPSVYLHALGMINALGGDLDSIVPALAAGRSPGMANAHTGIGEAFVGSVLAPLELAPR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
AELARYDCRNNRLLLAALAQIAPAVEAARERYGAHRVGVVLGTSTSGIEAAEAAFVYQAQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHEEEEEEC
AGDLPANFNYRQMEIGTAAPFAAAALGLHGPAFTISTACTSSAKAFASARRLLQLQLCDA
CCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
VVVGGVDSLCELTVQGFASLESTSNVRSNPMSRNRCGINVGEGAAVFLMSRDEAMVRVAG
HHHCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEECCCCEEEEEECCCEEEEEEE
VGESSDAHHISSPDPQGVGGELALRAALADAGVESAAIGYVNLHATATRKNDEMEAHLMS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEEEECCCCCHHHHHHHH
RVFPGGVPTSGTKPLTGHQLGAAGATELGFAWLTLARENVPLPRHLWDGEADPALPPLDL
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHH
VESERFLPQGGAQYAMSNSFAFGGSNVSLILAR
HCCCCCCCCCCCCHHCCCCEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]