Click here to switch to the map view.

The map label for this gene is algU [H]

Identifier: 91784714

GI number: 91784714

Start: 3702414

End: 3703013

Strand: Reverse

Name: algU [H]

Synonym: Bxe_A1078

Alternate gene names: 91784714

Gene position: 3703013-3702414 (Counterclockwise)

Preceding gene: 91784715

Following gene: 91784713

Centisome position: 75.64

GC content: 56.5

Gene sequence:

>600_bases
GTGAGCGAAAAAGAAATTGATCAGGTGCTGGTCGAGCGCGTCCAGAAGGGCGACAAGGCCGCGTTCGAGCTTCTGGTCTC
CAAATACCACCGCAAGATCCTCCGGCTGATCTCACGCCTCGTGCGGGACCCCGCCGAGGTGGAAGACGTCGCTCAGGACG
CCTTTATCAAGGCGTACCGGGCGTTGCCGCAGTTTCGCGGGGAGTCGGCCTTTTATACGTGGTTGTACCGGATTGCCGTC
AACACGGCAAAGAACTACCTTGCGACCCAAGGGCGGCGCGCGCCGACCTCGACCGAAGCAGATGCTGAAGAAGCTGAAAC
TTTCTCGGACGCGGACCAACTAAGGGATATCAACACGCCTGAGTCGATGTTGATGAGCAAGCAGATCGCTGAGACGGTCA
ATGCTGCGATGGCGGTTTTACCGGAAGAGTTGCGCACCGCCATTACTCTTCGTGAAATTGAAGGTTTGAGCTACGAGGAA
ATCGCTGAGATGATGGGTTGCCCAATCGGCACAGTCAGATCACGAATTTTCCGTGCTCGCGAAGCCATTGCGGCAAAATT
GCGTCCGTTACTTGACACACCTGAAGGCAAGCGCTGGTGA

Upstream 100 bases:

>100_bases
TGTAACGACTGTAACCGCTGTTACCGGCGTAACGTGCCCGGATGACGGGAACGCTACAATGGTCCCCCGCCATGCGCCCT
ATAGTTAACGGATTTATCAG

Downstream 100 bases:

>100_bases
ACGACCAGCCTGCCGGGCGGGGCGCGGGTGCGATATCTGGATTAGTGGTGTCACTACGGGGTATTTGTAAGATGGGGAGC
ATCATGGGGTCGGTCTCGAT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSEKEIDQVLVERVQKGDKAAFELLVSKYHRKILRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEE
IAEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW

Sequences:

>Translated_199_residues
MSEKEIDQVLVERVQKGDKAAFELLVSKYHRKILRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEE
IAEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW
>Mature_198_residues
SEKEIDQVLVERVQKGDKAAFELLVSKYHRKILRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAVN
TAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEEI
AEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=61.7801047120419, Blast_Score=237, Evalue=3e-64,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22581; Mature: 22450

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKEIDQVLVERVQKGDKAAFELLVSKYHRKILRLISRLVRDPAEVEDVAQDAFIKAYR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEEIAEMMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAAKLRPLLDTPEGKRW
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEKEIDQVLVERVQKGDKAAFELLVSKYHRKILRLISRLVRDPAEVEDVAQDAFIKAYR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEEIAEMMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAAKLRPLLDTPEGKRW
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]