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The map label for this gene is amnF

Identifier: 91784645

GI number: 91784645

Start: 3635592

End: 3636401

Strand: Reverse

Name: amnF

Synonym: Bxe_A1149

Alternate gene names: 91784645

Gene position: 3636401-3635592 (Counterclockwise)

Preceding gene: 91784646

Following gene: 91784644

Centisome position: 74.28

GC content: 62.35

Gene sequence:

>810_bases
GTGACACCACAACAACGCGAAGAGGCCGCTCAGTCTCTTTATCAGGCGATGCAAAGCGGCAAACCGATCGCGCCCCTGCG
CGACACGTTCCCGGACATGAACGTGGACGACGCTTACGCGATCCAGAGCATCAACACACAACGGCGAATCTCGCTGGGCC
GGCGCGTGGTCGGTCGCAAGATCGGCCTGACATCCGTCGTCGTTCAACAGCAACTCGGTGTGGACGAGCCCGACTTCGGT
GCTCTCTTCGATGACATGTCCTTTGGCGATGCCGAGACGATCCCTCTGTCGATTCTTCATCAACCGAAGGTCGAGGCAGA
AATCGGCTTTGTCCTCGGCCGCGACCTGGACACGGAGCAGCCTACGCACCAGGAGGTCCTGCAGGCTGTGGACTACGTGG
TTCCAGCGCTGGAAATCGTGGGTAGCCGCATCGCTGACTGGAACATCAAGTTCGTCGATACCGTGGCCGACAACGCTTCG
AGCGGAGTCTACGTGTTGGGATCGACGCCGATTTCTCCCCGTGGCCTGGACCTGAGCTTGGTAGGAATGTGCCTGTCGCG
CCGCGGGGAGCCGGTGTCTACCGGCGCAGGCGCGGCCTGCCTGGGCACCCCCCTGAACGCTGTGGTCTGGCTGGCGCGCA
CCATGTCCCGCCTGGGCAAGCCGCTGCGTGCCGGCGAACTCATCCTTTCGGGCGCGCTGGGCCCGATGGTCGCGGTCAAG
CCGGGCGACGTGTTCGAGTGCCACATCAATGGCGTGGGAAGCGTTCGCACAGAGTTTGAATCCAACCAAATGAATGGAGT
TGCGGCATGA

Upstream 100 bases:

>100_bases
CGGTGGCTGTCCATCAGCTGCCTCACCCGATGCTGCGCATCGAAATCAAGGCAGTGGCCTACAAGCCGCCGGCCCCAGAC
GCATAGAGGAAGGTTCTATC

Downstream 100 bases:

>100_bases
GCGTGCACGAAGAAATCATCATCCGCCTGGACGACGCCGCAAGATACGCGCACGAGGTCCCACAGTTCGACACCGACAAC
ACACTGAACCTGCCCGACGC

Product: 2-oxopent-4-enoate hydratase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase 3

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTPQQREEAAQSLYQAMQSGKPIAPLRDTFPDMNVDDAYAIQSINTQRRISLGRRVVGRKIGLTSVVVQQQLGVDEPDFG
ALFDDMSFGDAETIPLSILHQPKVEAEIGFVLGRDLDTEQPTHQEVLQAVDYVVPALEIVGSRIADWNIKFVDTVADNAS
SGVYVLGSTPISPRGLDLSLVGMCLSRRGEPVSTGAGAACLGTPLNAVVWLARTMSRLGKPLRAGELILSGALGPMVAVK
PGDVFECHINGVGSVRTEFESNQMNGVAA

Sequences:

>Translated_269_residues
MTPQQREEAAQSLYQAMQSGKPIAPLRDTFPDMNVDDAYAIQSINTQRRISLGRRVVGRKIGLTSVVVQQQLGVDEPDFG
ALFDDMSFGDAETIPLSILHQPKVEAEIGFVLGRDLDTEQPTHQEVLQAVDYVVPALEIVGSRIADWNIKFVDTVADNAS
SGVYVLGSTPISPRGLDLSLVGMCLSRRGEPVSTGAGAACLGTPLNAVVWLARTMSRLGKPLRAGELILSGALGPMVAVK
PGDVFECHINGVGSVRTEFESNQMNGVAA
>Mature_268_residues
TPQQREEAAQSLYQAMQSGKPIAPLRDTFPDMNVDDAYAIQSINTQRRISLGRRVVGRKIGLTSVVVQQQLGVDEPDFGA
LFDDMSFGDAETIPLSILHQPKVEAEIGFVLGRDLDTEQPTHQEVLQAVDYVVPALEIVGSRIADWNIKFVDTVADNASS
GVYVLGSTPISPRGLDLSLVGMCLSRRGEPVSTGAGAACLGTPLNAVVWLARTMSRLGKPLRAGELILSGALGPMVAVKP
GDVFECHINGVGSVRTEFESNQMNGVAA

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily

Homologues:

Organism=Escherichia coli, GI87081722, Length=261, Percent_Identity=57.8544061302682, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPD3_PSEPU (Q706S1)

Other databases:

- EMBL:   AJ617740
- HSSP:   P77608
- ProteinModelPortal:   Q706S1
- SMR:   Q706S1
- HAMAP:   MF_01655
- InterPro:   IPR002529
- InterPro:   IPR011234
- Gene3D:   G3DSA:3.90.850.10

Pfam domain/function: PF01557 FAA_hydrolase; SSF56529 Fumarylacetoacetase_C-rel

EC number: =4.2.1.80

Molecular weight: Translated: 28759; Mature: 28628

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQQREEAAQSLYQAMQSGKPIAPLRDTFPDMNVDDAYAIQSINTQRRISLGRRVVGRK
CCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHH
IGLTSVVVQQQLGVDEPDFGALFDDMSFGDAETIPLSILHQPKVEAEIGFVLGRDLDTEQ
CCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCC
PTHQEVLQAVDYVVPALEIVGSRIADWNIKFVDTVADNASSGVYVLGSTPISPRGLDLSL
CHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCHHH
VGMCLSRRGEPVSTGAGAACLGTPLNAVVWLARTMSRLGKPLRAGELILSGALGPMVAVK
HHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCEEEEC
PGDVFECHINGVGSVRTEFESNQMNGVAA
CCCEEEEEECCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPQQREEAAQSLYQAMQSGKPIAPLRDTFPDMNVDDAYAIQSINTQRRISLGRRVVGRK
CCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHH
IGLTSVVVQQQLGVDEPDFGALFDDMSFGDAETIPLSILHQPKVEAEIGFVLGRDLDTEQ
CCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCC
PTHQEVLQAVDYVVPALEIVGSRIADWNIKFVDTVADNASSGVYVLGSTPISPRGLDLSL
CHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCHHH
VGMCLSRRGEPVSTGAGAACLGTPLNAVVWLARTMSRLGKPLRAGELILSGALGPMVAVK
HHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCEEEEC
PGDVFECHINGVGSVRTEFESNQMNGVAA
CCCEEEEEECCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791097; 12823813