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The map label for this gene is amnE [H]

Identifier: 91784644

GI number: 91784644

Start: 3634831

End: 3635595

Strand: Reverse

Name: amnE [H]

Synonym: Bxe_A1150

Alternate gene names: 91784644

Gene position: 3635595-3634831 (Counterclockwise)

Preceding gene: 91784645

Following gene: 91784643

Centisome position: 74.26

GC content: 64.58

Gene sequence:

>765_bases
ATGAGCGTGCACGAAGAAATCATCATCCGCCTGGACGACGCCGCAAGATACGCGCACGAGGTCCCACAGTTCGACACCGA
CAACACACTGAACCTGCCCGACGCCTACGAGATCCAGCGCGGCTCCATCGCCCGTCGACTTGAGCGGGGCGAGCGGCGTG
TCGGCGTGAAGATGGGGTTCACCAGCCGGGCCAAGATGGTTCAGATGGGGCTGTCCGATGTCATCTGGGGCCGATTGACT
GACGCCATGCAATTGGAGGAGGGCTCCAATGTCGAGCATTCGCGTTTCGTGCATCCCCGGGTCGAGCCGGAGCTGGCATT
CCTGCTGAAAAAGCCTCTGGCGGGCAATGTCACCGGAGCCGAGGCGCTGGCGGCGGTCGAAGCGATTGCACCTGCTTTGG
AGGTGATCGACTCCCGCTATCAGAACTTCAAGTTCACGCTGCCGGAGGTCATCGCGGACAACGCGTCTTCCAGCGGCTTC
GTGGTTGGCGGCTGGCATTCGCCGGCCACGGACTTCGGCAACCTGGGGCTGGTCATGAGCCTGGACGGCCGCGCCGTGCA
GGTCGGCTCCACGGCGGCGCTGCTCGGCGATCCGCTGCGCTCGCTGGTCGCCGCAGCGCGGGTTTCGGCCGCTGCGAACG
AACCCTTGGAGGCCGGTTGGATCGTTCTGGCCGGGGGGGCGACGCCCGCGGAGTGGATCTATCCCGGTCAGCACATTTCG
ATGCGCATGCAGGGCTTGGGGAGCGTTAGCTTTCAACTGGTTTGA

Upstream 100 bases:

>100_bases
TGGTCGCGGTCAAGCCGGGCGACGTGTTCGAGTGCCACATCAATGGCGTGGGAAGCGTTCGCACAGAGTTTGAATCCAAC
CAAATGAATGGAGTTGCGGC

Downstream 100 bases:

>100_bases
TTGGGGTCTTGCGCGTCAAGCGCACCACCGCACTTGCAGCGTCTATATCTGGAGTTCTCTATGACCCGAAAATTCAAAGC
CGCCATCATCGGCAGCGGAA

Product: 4-oxalocrotonate decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSVHEEIIIRLDDAARYAHEVPQFDTDNTLNLPDAYEIQRGSIARRLERGERRVGVKMGFTSRAKMVQMGLSDVIWGRLT
DAMQLEEGSNVEHSRFVHPRVEPELAFLLKKPLAGNVTGAEALAAVEAIAPALEVIDSRYQNFKFTLPEVIADNASSSGF
VVGGWHSPATDFGNLGLVMSLDGRAVQVGSTAALLGDPLRSLVAAARVSAAANEPLEAGWIVLAGGATPAEWIYPGQHIS
MRMQGLGSVSFQLV

Sequences:

>Translated_254_residues
MSVHEEIIIRLDDAARYAHEVPQFDTDNTLNLPDAYEIQRGSIARRLERGERRVGVKMGFTSRAKMVQMGLSDVIWGRLT
DAMQLEEGSNVEHSRFVHPRVEPELAFLLKKPLAGNVTGAEALAAVEAIAPALEVIDSRYQNFKFTLPEVIADNASSSGF
VVGGWHSPATDFGNLGLVMSLDGRAVQVGSTAALLGDPLRSLVAAARVSAAANEPLEAGWIVLAGGATPAEWIYPGQHIS
MRMQGLGSVSFQLV
>Mature_253_residues
SVHEEIIIRLDDAARYAHEVPQFDTDNTLNLPDAYEIQRGSIARRLERGERRVGVKMGFTSRAKMVQMGLSDVIWGRLTD
AMQLEEGSNVEHSRFVHPRVEPELAFLLKKPLAGNVTGAEALAAVEAIAPALEVIDSRYQNFKFTLPEVIADNASSSGFV
VGGWHSPATDFGNLGLVMSLDGRAVQVGSTAALLGDPLRSLVAAARVSAAANEPLEAGWIVLAGGATPAEWIYPGQHISM
RMQGLGSVSFQLV

Specific function: Involved in the modified meta-cleavage pathway for the 2-aminophenol catabolism [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=223, Percent_Identity=33.1838565022422, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.1.1.77 [H]

Molecular weight: Translated: 27377; Mature: 27245

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHEEIIIRLDDAARYAHEVPQFDTDNTLNLPDAYEIQRGSIARRLERGERRVGVKMGF
CCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
TSRAKMVQMGLSDVIWGRLTDAMQLEEGSNVEHSRFVHPRVEPELAFLLKKPLAGNVTGA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHCCCCCCCCHH
EALAAVEAIAPALEVIDSRYQNFKFTLPEVIADNASSSGFVVGGWHSPATDFGNLGLVMS
HHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEE
LDGRAVQVGSTAALLGDPLRSLVAAARVSAAANEPLEAGWIVLAGGATPAEWIYPGQHIS
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCEE
MRMQGLGSVSFQLV
EHCCCCCCEEEEEC
>Mature Secondary Structure 
SVHEEIIIRLDDAARYAHEVPQFDTDNTLNLPDAYEIQRGSIARRLERGERRVGVKMGF
CCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
TSRAKMVQMGLSDVIWGRLTDAMQLEEGSNVEHSRFVHPRVEPELAFLLKKPLAGNVTGA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHCCCCCCCCHH
EALAAVEAIAPALEVIDSRYQNFKFTLPEVIADNASSSGFVVGGWHSPATDFGNLGLVMS
HHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEE
LDGRAVQVGSTAALLGDPLRSLVAAARVSAAANEPLEAGWIVLAGGATPAEWIYPGQHIS
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCEE
MRMQGLGSVSFQLV
EHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9169437; 11081795 [H]